基于电穿孔的巨芽孢杆菌转化系统的研制。

IF 1.3 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-09-23 DOI:10.1093/bbb/zbaf107

Taisuke Watanabe, Shinkuro Kimura, Takafumi Kasumi, Jun Ogihara

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引用次数: 0

摘要

我们为生产赤藓糖醇的担子菌酵母菌（Moniliella megachiliensis）开发了一种电孔转化系统。利用绿色荧光蛋白（GFP）表达载体，证实了基因的成功转移和表达。这是该属植物遗传转化的首次报道，为今后在食品和发酵工业中的基因工程和生物技术应用奠定了基础。

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Development of an electroporation-based transformation system for Moniliella megachiliensis.

We developed an electroporation-based transformation system for Moniliella megachiliensis, a basidiomycetous yeast producing erythritol. Using a green fluorescence protein expression vector, successful gene transfer and expression were confirmed. This is the first report of genetic transformation in this genus, providing a foundation for future genetic engineering and biotechnological applications in food and fermentation industries.

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来源期刊

Bioscience, Biotechnology, and Biochemistry 生物-生化与分子生物学

CiteScore

3.50

自引率

0.00%

发文量

183

审稿时长

1 months

期刊介绍： Bioscience, Biotechnology, and Biochemistry publishes high-quality papers providing chemical and biological analyses of vital phenomena exhibited by animals, plants, and microorganisms, the chemical structures and functions of their products, and related matters. The Journal plays a major role in communicating to a global audience outstanding basic and applied research in all fields subsumed by the Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA).