羧甲基田菁胶的结构、性能表征及其抗氧化活性评价

IF 10.7 1区 化学 Q1 CHEMISTRY, APPLIED
Xiaojia Bian , Kuaitian Wang , Na Zhang , Chen Wang , Donghui Geng , Yongqiang Cheng , Ning Tang
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引用次数: 0

摘要

羧甲基化是一种被广泛认可的修饰半乳甘露聚糖的方法,常用于提高其溶解度、稳定性和生物活性。本研究在不同的反应条件下合成了羧甲基田菁胶(CMSG),并系统评价了这些条件对取代度的影响。碱性条件对羧甲基化至关重要,反应温度、时间和醚化剂浓度对取代度有显著影响。在碱摩尔比为6.885,醚化剂摩尔比为3.43的条件下,在60°C下碱化4小时或50°C下碱化9小时,在30°C碱化1小时后,最大取代度为0.91。结构分析证实了成功的修饰,并发现了非选择性的羧甲基取代,同时由于氢键破坏导致分子量和热稳定性降低。值得注意的是,CMSG对Caco-2细胞的保护作用增强;取代度越高,抗氧化活性越强。这种活性的提高是由于分子量降低和电荷密度增加,导致细胞内超氧化物歧化酶和谷胱甘肽过氧化物酶水平升高,从而减少活性氧和脂质过氧化。这些发现突出了CMSG作为一种具有增强抗氧化性能的功能性生物材料的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Characterization of the structure and properties of carboxymethyl sesbania gum and its antioxidant activity evaluation
Carboxymethylation is a widely recognized method for modifying galactomannans, often employed to enhance their solubility, stability, and biological activities. In this study, carboxymethyl sesbania gum (CMSG) was synthesized under different reaction conditions, and the influence of these conditions on the degree of substitution was systematically assessed. Alkaline conditions were crucial for carboxymethylation, with reaction temperature, time, and etherifying agent concentration significantly affecting the degree of substitution. The maximum degree of substitution of 0.91 was achieved using an alkali molar ratio of 6.885 and an etherifying agent ratio of 3.43, under either 60 °C for 4 h or 50 °C for 9 h, following alkalization at 30 °C for 1 h. Structural analysis confirmed successful modification and revealed non-selective carboxymethyl group substitution, along with a reduction in molecular weight and thermal stability due to hydrogen bond disruption. Notably, CMSG demonstrated enhanced protective effects on Caco-2 cells; higher degrees of substitution correlated with superior antioxidant activity. This improved activity is attributed to lower molecular weight and increased charge density, leading to elevated intracellular superoxide dismutase and glutathione peroxidase levels, consequently reducing reactive oxygen species and lipid peroxidation. These findings highlight the potential of CMSG as a functional biomaterial with enhanced antioxidant properties.
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来源期刊
Carbohydrate Polymers
Carbohydrate Polymers 化学-高分子科学
CiteScore
22.40
自引率
8.00%
发文量
1286
审稿时长
47 days
期刊介绍: Carbohydrate Polymers stands as a prominent journal in the glycoscience field, dedicated to exploring and harnessing the potential of polysaccharides with applications spanning bioenergy, bioplastics, biomaterials, biorefining, chemistry, drug delivery, food, health, nanotechnology, packaging, paper, pharmaceuticals, medicine, oil recovery, textiles, tissue engineering, wood, and various aspects of glycoscience. The journal emphasizes the central role of well-characterized carbohydrate polymers, highlighting their significance as the primary focus rather than a peripheral topic. Each paper must prominently feature at least one named carbohydrate polymer, evident in both citation and title, with a commitment to innovative research that advances scientific knowledge.
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