溶菌酶功能化氧化锌纳米颗粒：合成、表征和对金黄色葡萄球菌的抗菌评估及其在废水监测中的未来应用

Next Nanotechnology Pub Date : 2025-01-01 DOI:10.1016/j.nxnano.2025.100222

Suneeti Singh, Pavni Rekhi, Jewel Mary Saju

{"title":"溶菌酶功能化氧化锌纳米颗粒：合成、表征和对金黄色葡萄球菌的抗菌评估及其在废水监测中的未来应用","authors":"Suneeti Singh, Pavni Rekhi, Jewel Mary Saju","doi":"10.1016/j.nxnano.2025.100222","DOIUrl":null,"url":null,"abstract":"<div><div>Access to clean drinking water remains a global concern, primarily due to the presence of biological, inorganic, and organic pollutants. Among the biological contaminants, waterborne pathogens pose a significant threat to human well-being. Since nanoparticle-based biosensors have a higher sensitivity for detecting bacteria than conventional detection techniques, they have become increasingly prevalent. In this context, the current study reports zinc oxide nanoparticles functionalized with lysozyme (ZnO@LY NPs) via a modified oxidation-reduction mechanism, followed by an assessment of their antibacterial activity. The morphology and size of the functionalized ZnO@LY NPs were determined using Transmission electron microscopy-energy dispersive X-ray spectroscopy (TEM-EDX), X-ray diffraction (XRD), dynamic light Scattering (DLS), and zeta potential analysis, which revealed nanoparticles of size between 14 and 32 nm with + 31.1 mV charge. Further, the functionalization of ZnO with lysozyme was confirmed using Fourier-transform infrared spectroscopy (FTIR) and Ultraviolet-Visible (UV-Vis) spectroscopy. The antibacterial efficacy of ZnO@LY NPs and ZnO NPs against Gram-positive bacteria (<em>Staphylococcus aureus</em>) was assessed in a comparative study. After 30 min and 24 h of treatment with ZnO@LY NPs at 250 ppm, the agar spot assay revealed the absence of colonies. Similarly, ZnO@LY NPs exhibited a 25 % stronger and statistically significant antibacterial impact than non-functionalized ZnO NPs in the bacterial growth inhibition assay, as confirmed by repeated measures (RM)-ANOVA analysis. Moreover, Dunnett's multiple comparison test revealed that ZnO@LY NPs showed a dose-dependent linear response when compared to ZnO NPs. The superior antibacterial performance of ZnO@LY NPs is attributed to improved bacterial surface interactions, stability, and biocompatibility facilitated by lysozyme functionalization. These findings suggest a potential application of ZnO@LY nanoparticles in biosensors for on-site pathogen detection in wastewater.</div></div>","PeriodicalId":100959,"journal":{"name":"Next Nanotechnology","volume":"8 ","pages":"Article 100222"},"PeriodicalIF":0.0000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Lysozyme functionalized zinc-oxide nanoparticles: Synthesis, characterization, and antibacterial assessment against Staphylococcus aureus with futuristic application in wastewater monitoring\",\"authors\":\"Suneeti Singh, Pavni Rekhi, Jewel Mary Saju\",\"doi\":\"10.1016/j.nxnano.2025.100222\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Access to clean drinking water remains a global concern, primarily due to the presence of biological, inorganic, and organic pollutants. Among the biological contaminants, waterborne pathogens pose a significant threat to human well-being. Since nanoparticle-based biosensors have a higher sensitivity for detecting bacteria than conventional detection techniques, they have become increasingly prevalent. In this context, the current study reports zinc oxide nanoparticles functionalized with lysozyme (ZnO@LY NPs) via a modified oxidation-reduction mechanism, followed by an assessment of their antibacterial activity. The morphology and size of the functionalized ZnO@LY NPs were determined using Transmission electron microscopy-energy dispersive X-ray spectroscopy (TEM-EDX), X-ray diffraction (XRD), dynamic light Scattering (DLS), and zeta potential analysis, which revealed nanoparticles of size between 14 and 32 nm with + 31.1 mV charge. Further, the functionalization of ZnO with lysozyme was confirmed using Fourier-transform infrared spectroscopy (FTIR) and Ultraviolet-Visible (UV-Vis) spectroscopy. The antibacterial efficacy of ZnO@LY NPs and ZnO NPs against Gram-positive bacteria (<em>Staphylococcus aureus</em>) was assessed in a comparative study. After 30 min and 24 h of treatment with ZnO@LY NPs at 250 ppm, the agar spot assay revealed the absence of colonies. Similarly, ZnO@LY NPs exhibited a 25 % stronger and statistically significant antibacterial impact than non-functionalized ZnO NPs in the bacterial growth inhibition assay, as confirmed by repeated measures (RM)-ANOVA analysis. Moreover, Dunnett's multiple comparison test revealed that ZnO@LY NPs showed a dose-dependent linear response when compared to ZnO NPs. The superior antibacterial performance of ZnO@LY NPs is attributed to improved bacterial surface interactions, stability, and biocompatibility facilitated by lysozyme functionalization. These findings suggest a potential application of ZnO@LY nanoparticles in biosensors for on-site pathogen detection in wastewater.</div></div>\",\"PeriodicalId\":100959,\"journal\":{\"name\":\"Next Nanotechnology\",\"volume\":\"8 \",\"pages\":\"Article 100222\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Next Nanotechnology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2949829525000919\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Next Nanotechnology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2949829525000919","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

摘要

获得清洁饮用水仍然是全球关注的问题，主要是由于生物、无机和有机污染物的存在。在生物污染物中，水传播病原体对人类健康构成重大威胁。由于基于纳米粒子的生物传感器在检测细菌方面具有比传统检测技术更高的灵敏度，因此它们变得越来越普遍。在此背景下，目前的研究报道了氧化锌纳米颗粒通过改进的氧化还原机制与溶菌酶（ZnO@LY NPs）功能化，随后评估了它们的抗菌活性。利用透射电子显微镜-能量色散x射线能谱（TEM-EDX）、x射线衍射（XRD）、动态光散射（DLS）和zeta电位分析对功能化ZnO@LY纳米粒子的形貌和尺寸进行了测定，发现纳米粒子的尺寸在14 ~ 32 nm之间，电荷为+ 31.1 mV。此外，利用傅里叶红外光谱（FTIR）和紫外可见光谱（UV-Vis）证实了氧化锌与溶菌酶的功能化。比较研究了ZnO@LY NPs和ZnO NPs对革兰氏阳性菌（金黄色葡萄球菌）的抑菌效果。在250 ppm浓度的ZnO@LY NPs处理30 min和24 h后，琼脂斑点实验显示没有菌落。同样，通过重复测量(RM)-方差分析，ZnO@LY NPs在细菌生长抑制实验中表现出比非功能化ZnO NPs强25 %且具有统计学意义的抗菌效果。此外，Dunnett的多重比较测试表明，ZnO@LY NPs与ZnO NPs相比表现出剂量依赖性的线性响应。ZnO@LY NPs优越的抗菌性能归功于通过溶菌酶功能化改善的细菌表面相互作用、稳定性和生物相容性。这些发现提示ZnO@LY纳米颗粒在废水中现场病原体检测的生物传感器中的潜在应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Lysozyme functionalized zinc-oxide nanoparticles: Synthesis, characterization, and antibacterial assessment against Staphylococcus aureus with futuristic application in wastewater monitoring

Access to clean drinking water remains a global concern, primarily due to the presence of biological, inorganic, and organic pollutants. Among the biological contaminants, waterborne pathogens pose a significant threat to human well-being. Since nanoparticle-based biosensors have a higher sensitivity for detecting bacteria than conventional detection techniques, they have become increasingly prevalent. In this context, the current study reports zinc oxide nanoparticles functionalized with lysozyme (ZnO@LY NPs) via a modified oxidation-reduction mechanism, followed by an assessment of their antibacterial activity. The morphology and size of the functionalized ZnO@LY NPs were determined using Transmission electron microscopy-energy dispersive X-ray spectroscopy (TEM-EDX), X-ray diffraction (XRD), dynamic light Scattering (DLS), and zeta potential analysis, which revealed nanoparticles of size between 14 and 32 nm with + 31.1 mV charge. Further, the functionalization of ZnO with lysozyme was confirmed using Fourier-transform infrared spectroscopy (FTIR) and Ultraviolet-Visible (UV-Vis) spectroscopy. The antibacterial efficacy of ZnO@LY NPs and ZnO NPs against Gram-positive bacteria (Staphylococcus aureus) was assessed in a comparative study. After 30 min and 24 h of treatment with ZnO@LY NPs at 250 ppm, the agar spot assay revealed the absence of colonies. Similarly, ZnO@LY NPs exhibited a 25 % stronger and statistically significant antibacterial impact than non-functionalized ZnO NPs in the bacterial growth inhibition assay, as confirmed by repeated measures (RM)-ANOVA analysis. Moreover, Dunnett's multiple comparison test revealed that ZnO@LY NPs showed a dose-dependent linear response when compared to ZnO NPs. The superior antibacterial performance of ZnO@LY NPs is attributed to improved bacterial surface interactions, stability, and biocompatibility facilitated by lysozyme functionalization. These findings suggest a potential application of ZnO@LY nanoparticles in biosensors for on-site pathogen detection in wastewater.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Next Nanotechnology

自引率

0.00%

发文量