桧木醇通过诱导细胞凋亡和抑制细胞迁移，在人口腔鳞状细胞癌中显示出强大的抗癌作用

IF 0.4 Q4 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral and Maxillofacial Surgery Medicine and Pathology Pub Date : 2025-03-07 DOI:10.1016/j.ajoms.2025.02.019

Hideki Yoshimatsu , Ryuhei Kanda , Lyu Xinghui , Keita Kano

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引用次数: 0

摘要

目的研究表明，扁桃木醇（HNK）对多种疾病具有抗炎、抗菌等多种药理作用。然而，它对口腔癌的影响还没有报道。因此，我们研究了HNK在口腔鳞状细胞癌（OSCC）中的抗癌活性及其作用机制。方法采用MTT法检测细胞活力，DAPI染色、TUNEL染色、膜联蛋白V/碘化丙啶（PI）染色、流式细胞术检测细胞凋亡。使用伤口愈合试验评估细胞迁移能力。结果HNK抑制HSC-2细胞增殖呈剂量依赖性。此外，我们的评估显示HNK通过诱导细胞凋亡发挥其抗癌作用。此外，HNK以剂量依赖性的方式抑制HSC-2细胞的迁移。结论HNK具有作为治疗OSCC的重要先导化合物的潜力。此外，HNK通过诱导细胞凋亡和抑制细胞迁移来抑制OSCC。

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Hinokitiol exhibits potent anticancer effects in human oral squamous cell carcinoma by inducing apoptosis and inhibition of cell migration

Objective

Hinokitiol (HNK) has been shown to have various pharmacological effects, including anti-inflammatory and anti-bacterial activities, against various diseases. However, its effects on oral cancer have not been reported. Therefore, we investigated the anti-cancer activity and mechanism of action of HNK in oral squamous cell carcinoma (OSCC).

Methods

Cell viability was measured using the MTT assay, while apoptosis was assessed through DAPI staining, TUNEL assay, annexin V/propidium iodide (PI) staining, and flow cytometry. Cell migration capacity was evaluated using a wound-healing assay.

Results

Our findings indicate that HNK inhibits the proliferation of HSC-2 cells in a dose-dependent manner. Additionally, our evaluation revealed that HNK exerts its anticancer effects by inducing apoptosis. Moreover, HNK inhibited the migration of HSC-2 cells in a dose-dependent manner.

Conclusions

Based on our results, we conclude that HNK has the potential as an important lead compound in treating OSCC. Moreover, HNK inhibits OSCC by inducing apoptosis and suppressing cell migration.

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