复制因子C亚单位5 （RFC5）在头颈部鳞状细胞癌中表达的临床、预后和免疫学影响的鉴定

IF 0.4 Q4 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral and Maxillofacial Surgery Medicine and Pathology Pub Date : 2025-04-27 DOI:10.1016/j.ajoms.2025.04.014

Dakshitha Sankar , Anitha Pandi , Palati Sinduja , Vijayashree Priyadharsini Jayaseelan , Paramasivam Arumugam

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引用次数: 0

摘要

目的鉴定和验证复制因子C亚基5 (RFC5) mRNA和蛋白在头颈部鳞状细胞癌（HNSCC）患者中的表达，分析其与临床病理特征、预后和免疫特征的关系。方法本研究主要依靠癌症基因组图谱（TCGA-HNSCC）数据集，另外招募了32例OSCC患者，OSCC是HNSCC中常见的一种癌症。采用real - time-qPCR和Western blot等方法分析口腔鳞癌（OSCC）及癌旁正常组织中RFC5 mRNA和蛋白的表达。使用TCGA-HNSCC数据集分析RFC5表达在临床病理特征、生存、免疫调节和功能富集分析中的意义。结果rfc5 mRNA和蛋白在多种肿瘤中表达显著改变，在HNSCC/OSCC中表达上调。RFC5 mRNA表达与癌症分期、分级、淋巴结转移、HPV状态和不良预后相关，提示其作为生物标志物的潜力。蛋白质网络分析确定了RFC5与参与DNA复制和癌症途径的各种已知癌蛋白之间的相互作用。功能富集分析显示，RFC5与HNSCC的发生发展密切相关。结论srfc5在HNSCC中起关键作用，其过表达与侵袭性临床特征和不良预后相关。它在致癌途径中的作用以及与关键蛋白的相互作用表明它具有作为生物标志物和治疗靶点的潜力。

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Identification of clinical, prognostic and immunological impact of replication factor C subunit 5 (RFC5) expression in head and neck squamous cell carcinoma

Objectives

This study aimed to identify and validate replication factor C subunit 5 (RFC5) mRNA and protein expression in head and neck squamous cell carcinoma (HNSCC) patients, analyzing its association with clinicopathological features, prognosis, and immune signatures.

Methods

The study primarily relied on the cancer genome atlas (TCGA-HNSCC) dataset and additionally recruited 32 OSCC patients, a common type of cancer in HNSCC. RFC5 mRNA and protein expression were analyzed in oral squamous cell carcinoma (OSCC) and adjacent normal tissues using methods such as real time-qPCR and Western blot. The implications of RFC5 expression in clinicopathological features, survival, immune regulation, and functional enrichment analysis were analyzed using the TCGA-HNSCC dataset.

Results

RFC5 mRNA and protein are significantly altered in multiple cancers, particularly upregulated in HNSCC/OSCC. RFC5 mRNA expression is associated with cancer stage, grade, nodal metastasis, HPV status, and poor prognosis, suggesting its potential as a biomarker. Protein network analysis identified an interaction between RFC5 and various well-known oncoproteins involved in DNA replication and cancer pathways. Functional enrichment analysis showed a strong association between RFC5 and HNSCC development and progression.

Conclusions

RFC5 is a key player in HNSCC, and its overexpression is associated with aggressive clinical features and poor outcomes. Its role in oncogenic pathways and interaction with key proteins suggest its potential as a biomarker and therapeutic target.

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