Synthesis, characterization and biological investigations of novel phenylthiosemicarbazones

In this study the synthesis of novel Phenylthiosemicarbazones were obtained via condensation reaction of 4-Phenylthiosemicarbazide and p–substituted Isonitrosoacetophenones. The structural elucidation have been carried on the basis of Elemental analysis, UV–Visible, ¹H NMR, ¹³C NMR, FTIR and Mass spectral studies. The antimicrobial inhibitory action of the present compounds assessed against selected bacterial and fungal strains. Additionally, the Brine Shrimp Lethality Test (BSLT) has been used to screen these compounds for cytotoxic activity by determining the LC50 value. The obtained LC50 value of compounds L1-L6 was found to be < 1000 ppm. The synthesized compounds exhibit toxicity towards Artemia Salina Leach larvae and are believed to possess anticancer properties, Compound L5 demonstrated the highest level of toxicity, with an LC50 value of 51.65 mg/L. Anticancer screening was determined against triple negative human breast cancer cell line MDA-MB-231. In vitro cytotoxicity of the compounds was evaluated relative to paclitaxel using an MTT assay.