{"title":"一代six4 -nlsRFP：用于实时成像果蝇性腺发育的红色体细胞性腺核标记物。","authors":"Everette Rhymer, Lauren Anllo","doi":"10.17912/micropub.biology.001652","DOIUrl":null,"url":null,"abstract":"<p><p>Intrinsically fluorescent tissue labels are valuable to live image cell behaviors during development. Fluorescent markers of <i>Drosophila</i> gonadal cells have elucidated dynamic cellular interactions that shaped our conception of cell biology. Many green fluorescent protein (GFP) reagents are available to visualize cytoskeletal dynamics, extracellular matrix, and cell adhesion proteins. To facilitate use of these reagents while studying somatic gonadal development, we generated a somatic gonadal precursor (SGP) intrinsic red fluorescent nuclear marker. This tool can track SGP movement live, facilitating the study of somatic cell interactions required for gonadogenesis.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12264712/pdf/","citationCount":"0","resultStr":"{\"title\":\"Generation of <i>six4</i> -nlsRFP: a red somatic gonadal nuclear marker for live imaging <i>Drosophila</i> gonadogenesis.\",\"authors\":\"Everette Rhymer, Lauren Anllo\",\"doi\":\"10.17912/micropub.biology.001652\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Intrinsically fluorescent tissue labels are valuable to live image cell behaviors during development. Fluorescent markers of <i>Drosophila</i> gonadal cells have elucidated dynamic cellular interactions that shaped our conception of cell biology. Many green fluorescent protein (GFP) reagents are available to visualize cytoskeletal dynamics, extracellular matrix, and cell adhesion proteins. To facilitate use of these reagents while studying somatic gonadal development, we generated a somatic gonadal precursor (SGP) intrinsic red fluorescent nuclear marker. This tool can track SGP movement live, facilitating the study of somatic cell interactions required for gonadogenesis.</p>\",\"PeriodicalId\":74192,\"journal\":{\"name\":\"microPublication biology\",\"volume\":\"2025 \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12264712/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"microPublication biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.17912/micropub.biology.001652\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"microPublication biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17912/micropub.biology.001652","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
Generation of six4 -nlsRFP: a red somatic gonadal nuclear marker for live imaging Drosophila gonadogenesis.
Intrinsically fluorescent tissue labels are valuable to live image cell behaviors during development. Fluorescent markers of Drosophila gonadal cells have elucidated dynamic cellular interactions that shaped our conception of cell biology. Many green fluorescent protein (GFP) reagents are available to visualize cytoskeletal dynamics, extracellular matrix, and cell adhesion proteins. To facilitate use of these reagents while studying somatic gonadal development, we generated a somatic gonadal precursor (SGP) intrinsic red fluorescent nuclear marker. This tool can track SGP movement live, facilitating the study of somatic cell interactions required for gonadogenesis.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
