快速检测作为一种实际替代玻片凝集法用于确认霍乱弧菌O1。

IF 5.4 2区 医学 Q1 MICROBIOLOGY
Journal of Clinical Microbiology Pub Date : 2025-08-13 Epub Date: 2025-07-17 DOI:10.1128/jcm.00433-25
Piyash Bhattacharjee, Sonia T Hegde, Ashraful Islam Khan, Imrul Kayes Nabil, Md Naiem Hossain, Tahira Ahmed Rashmi, Mokibul Hassan Afrad, Md Taufiqul Islam, Mohammad Ashraful Amin, Zahid Hasan Khan, Taufiqur Rahman Bhuiyan, Andrew S Azman, Firdausi Qadri
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引用次数: 0

摘要

用于血清组和/或血清型鉴定的玻片凝集是通过培养确认霍乱的关键步骤。快速诊断测试(RDTs)通常直接用于粪便,但被认为不足以确认霍乱。然而,它们可能提供了一种实用的替代方法,以代替滑动凝集培养步骤,因为它们易于使用、储存,并且需要最少的培训。本研究利用rdt玻片凝集法对霍乱弧菌O1/O139的推定菌落检测结果进行一致性评价。纳入了达卡icddr b医院急性水样腹泻患者(≥1年)。粪便样本在硫代硫酸盐-柠檬酸盐-胆汁盐-蔗糖培养基上培养,在明胶琼脂培养基上继代培养推定菌落。然后用载玻片凝集和4个商业霍乱rdt对分离株进行检测。从2024年2月4日至2025年5月24日,纳入了1638例急性水样腹泻患者,1140例(70%)疑似有霍乱弧菌菌落,482例(29%)经培养确认为霍乱弧菌o型。我们使用Cholkit (n= 1140)、SD Bioline (n=693)、Crystal VC O1/O139 (n=693)和Crystal VC O1 (n=655) RDT试剂盒检测疑似霍乱弧菌菌落。RDTs显示与玻片凝集接近完美的一致性。以玻片凝集为对照,试剂盒的灵敏度为99.3% ~ 99.6%,特异性为99.3% ~ 99.7%。在典型的霍乱培养方案中,rdt提供了一种实用且可能更容易的替代方法,可以对假定的霍乱弧菌菌落进行玻片凝集。这可能有助于在实验室设施和试剂可能有限的环境中提供快速确认疫情的途径。重要意义本研究表明,快速诊断试验(RDTs)可以有效取代传统的玻片凝集方法,以确认属于O1血清群的疑似霍乱弧菌菌落,O1血清群是导致当前第七次霍乱大流行的原因。将四种RDTs与玻片凝集进行比较,我们发现几乎完全一致,敏感性和特异性超过99%。由于快速诊断疗法更便宜、更容易使用和储存,并且需要较少的技术培训,采用它们可以显著加快霍乱疫情的确认,特别是在实验室资源有限的地区。这一实际转变可能导致对霍乱疫情做出更快的反应,最终改善全球公共卫生监测和疾病控制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Rapid tests as a practical alternative to slide agglutination for the confirmation of V. cholerae O1.

Slide agglutination for serogroup and/or serotype identification is a crucial step for confirming cholera by culture. Rapid diagnostic tests (RDTs) are typically used directly on stool but are not considered sufficient for cholera confirmation. However, they may provide a practical alternative to the slide agglutination step of culture, as they are easy to use, store, and require minimal training. This study evaluates the concordance of Vibrio cholerae O1/O139 detection from presumptive colonies by slide agglutination with RDTs. Patients (≥1 year) with acute watery diarrhea at the icddr,b Dhaka hospital were enrolled. Stool samples were cultured on thiosulfate-citrate-bile salts-sucrose medium, and presumptive colonies were subcultured on Gelatin Agar medium. Isolates were then tested by slide agglutination and four commercial cholera RDTs. From 4 February 2024 through 24 May 2025, 1,638 patients with acute watery diarrhea were enrolled, 1,140 (70%) had presumptive V. cholerae colonies, and 482 (29%) were culture-confirmed as V. cholerae O1. We tested suspected V. cholerae colonies using Cholkit (n=1,140), SD Bioline (n=693), Crystal VC O1/O139 (n=693), and Crystal VC O1 (n=655) RDT kits. RDTs showed near-perfect concordance with slide agglutination. Using slide agglutination as the reference, the sensitivity of the RDT kits ranged from 99.3% to 99.6% and the specificity from 99.3% to 99.7%. RDTs offer a practical and potentially easier alternative to slide agglutination of presumptive V. cholerae colonies within typical cholera culture protocols. This may help to provide a pathway to quick confirmation of outbreaks in settings where lab facilities and reagents may be limited.IMPORTANCEThis study demonstrated that rapid diagnostic tests (RDTs) can effectively replace traditional slide agglutination methods to confirm suspected Vibrio cholerae colonies belonging to the O1 serogroup, which are responsible for the current seventh cholera pandemic. Comparing four RDTs to slide agglutination, we found almost perfect agreement, with sensitivity and specificity exceeding 99%. Since RDTs are cheaper, easier to use and store, and require less technical training, adopting them could significantly speed up cholera outbreak confirmation, especially in areas with limited laboratory resources. This practical shift could lead to faster responses to cholera outbreaks, ultimately improving public health surveillance and disease control globally.

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来源期刊
Journal of Clinical Microbiology
Journal of Clinical Microbiology 医学-微生物学
CiteScore
17.10
自引率
4.30%
发文量
347
审稿时长
3 months
期刊介绍: The Journal of Clinical Microbiology® disseminates the latest research concerning the laboratory diagnosis of human and animal infections, along with the laboratory's role in epidemiology and the management of infectious diseases.
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