植物病原真菌指状青霉中一种新型有丝分裂病毒的全基因组分析。

IF 2.5 4区医学 Q3 VIROLOGY

Archives of Virology Pub Date : 2025-07-17 DOI:10.1007/s00705-025-06364-9

Zhimin Tang, Yunhui He, Ya Yang, Xiaoping Zhang, Rui Zhou, Kang Zhou

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引用次数: 0

摘要

柑橘是全球种植最广泛、经济最重要的水果作物之一。然而，采后病原菌指状青霉在柑橘果实中引起绿霉腐烂，造成重大经济损失。本文从植物病原真菌digitatum青霉菌（Penicillium digitatum）中分离到一种新型分枝病毒PdMV1 (Penicillium digitatum mitovirus 1)，并测定了其分子特征。PdMV1基因组长度为2517 nt， a +U含量为62.7%。该ORF编码一个约734个氨基酸的RNA依赖性RNA聚合酶（RdRp），分子量为84.116 kDa。对RdRp序列进行BLASTp比较，结果显示RdRp序列与菌核核病毒32的相似性最高（同源性为65.01%）。系统发育分析表明，PdMV1是有丝分裂病毒科unuamitvirus属的新成员。据我们所知，这是首例有丝分裂病毒感染指状青霉菌的报道。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Complete genome analysis of a novel mitovirus in the phytopathogenic fungus Penicillium digitatum

Citrus is one of the most widely cultivated and economically important fruit crops globally. However, the postharvest pathogen Penicillium digitatum causes green mold decay in citrus fruits, leading to significant economic losses. Here, a novel mycovirus, Penicillium digitatum mitovirus 1 (PdMV1), was isolated from the phytopathogenic fungus P. digitatum, and its molecular characteristics were determined. PdMV1 has a genome that is 2,517 nt in length with an A+U content of 62.7%. The single ORF encodes a putative RNA-dependent RNA polymerase (RdRp) of 734 amino acids with a molecular mass of 84.116 kDa. A BLASTp comparison of the RdRp sequence showed the highest similarity (65.01% identity) to that of Sclerotinia sclerotiorum mitovirus 32. Phylogenetic analysis revealed that PdMV1 is a new member of the genus Unuamitovirus within the family Mitoviridae. To the best of our knowledge, this is the first report of a mitovirus infecting Penicillium digitatum.

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来源期刊

Archives of Virology 医学-病毒学

CiteScore

5.10

自引率

7.40%

发文量

324

审稿时长

4.5 months

期刊介绍： Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.