以腰果甘蔗渣为载体固定化磁木质素为载体的南极念珠菌脂肪酶B催化合成生物燃料酯

IF 2.9 4区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Hilldyson Moreira Levy, Tigressa Helena Soares Rodrigues, Tiago Lima de Albuquerque, Hosiberto Batista Sant’Ana, Filipe Xavier Feitosa, Maria Valderez Ponte Rocha
{"title":"以腰果甘蔗渣为载体固定化磁木质素为载体的南极念珠菌脂肪酶B催化合成生物燃料酯","authors":"Hilldyson Moreira Levy,&nbsp;Tigressa Helena Soares Rodrigues,&nbsp;Tiago Lima de Albuquerque,&nbsp;Hosiberto Batista Sant’Ana,&nbsp;Filipe Xavier Feitosa,&nbsp;Maria Valderez Ponte Rocha","doi":"10.1002/bbb.2793","DOIUrl":null,"url":null,"abstract":"<p>This study investigated the biosynthesis of ethyl oleate (EO), the main constituent of biodiesel, via the esterification of oleic acid with ethanol, catalyzed by <i>Candida antarctica</i> lipase B (CALB) immobilized on a low-cost support derived from cashew apple bagasse (CAB) lignin. Cashew apple bagasse lignin extraction was carried out through an acid-alkali process and used to produce the CAB-lignin conjugated with magnetite (Lig-MNP) composite, which served as the support for enzyme immobilization. The biocatalyst (Lig-MNP/CALB), obtained through the immobilization process, exhibited an activity of 13 U g<sup>−1</sup> and was subsequently applied in esterification reactions to evaluate its catalytic efficiency in comparison to the free enzyme. Acid-to-alcohol molar ratio, temperature, and catalytic load were evaluated. Under optimal conditions – 40 mg mL<sup>−1</sup> catalyst load, 40 °C, and a 1:2 molar ratio – Lig-MNP/CALB achieved a maximum EO conversion of 90%. The immobilized enzyme demonstrated operational stability over multiple cycles and was easily recovered through magnetic separation. The purified biofuel reached approximately 90% purity in ethyl oleate and 98% in total esters content. These results highlight the potential of the biocatalyst for sustainable biofuel production.</p>","PeriodicalId":55380,"journal":{"name":"Biofuels Bioproducts & Biorefining-Biofpr","volume":"19 4","pages":"1165-1173"},"PeriodicalIF":2.9000,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bbb.2793","citationCount":"0","resultStr":"{\"title\":\"Synthesis of biofuel esters catalyzed by lipase B from Candida antarctica immobilized on a magnetic lignin-based support derived from cashew apple bagasse\",\"authors\":\"Hilldyson Moreira Levy,&nbsp;Tigressa Helena Soares Rodrigues,&nbsp;Tiago Lima de Albuquerque,&nbsp;Hosiberto Batista Sant’Ana,&nbsp;Filipe Xavier Feitosa,&nbsp;Maria Valderez Ponte Rocha\",\"doi\":\"10.1002/bbb.2793\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>This study investigated the biosynthesis of ethyl oleate (EO), the main constituent of biodiesel, via the esterification of oleic acid with ethanol, catalyzed by <i>Candida antarctica</i> lipase B (CALB) immobilized on a low-cost support derived from cashew apple bagasse (CAB) lignin. Cashew apple bagasse lignin extraction was carried out through an acid-alkali process and used to produce the CAB-lignin conjugated with magnetite (Lig-MNP) composite, which served as the support for enzyme immobilization. The biocatalyst (Lig-MNP/CALB), obtained through the immobilization process, exhibited an activity of 13 U g<sup>−1</sup> and was subsequently applied in esterification reactions to evaluate its catalytic efficiency in comparison to the free enzyme. Acid-to-alcohol molar ratio, temperature, and catalytic load were evaluated. Under optimal conditions – 40 mg mL<sup>−1</sup> catalyst load, 40 °C, and a 1:2 molar ratio – Lig-MNP/CALB achieved a maximum EO conversion of 90%. The immobilized enzyme demonstrated operational stability over multiple cycles and was easily recovered through magnetic separation. The purified biofuel reached approximately 90% purity in ethyl oleate and 98% in total esters content. These results highlight the potential of the biocatalyst for sustainable biofuel production.</p>\",\"PeriodicalId\":55380,\"journal\":{\"name\":\"Biofuels Bioproducts & Biorefining-Biofpr\",\"volume\":\"19 4\",\"pages\":\"1165-1173\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2025-05-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bbb.2793\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biofuels Bioproducts & Biorefining-Biofpr\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/bbb.2793\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biofuels Bioproducts & Biorefining-Biofpr","FirstCategoryId":"5","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/bbb.2793","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

以腰果甘蔗渣(CAB)木质素为载体,固定化南极假丝酵母脂肪酶B (CALB),通过油酸与乙醇酯化反应合成生物柴油的主要成分油酸乙酯(EO)。采用酸碱法提取腰果甘蔗渣木质素,制备了磁铁矿偶联木质素复合材料(cab -木质素- mnp),作为固定化酶的载体。通过固定化过程获得的生物催化剂(li - mnp /CALB)的活性为13 U g−1,随后将其应用于酯化反应中,与游离酶进行比较,以评估其催化效率。对酸醇摩尔比、温度和催化负荷进行了评价。在最佳条件下- 40 mg mL−1催化剂负载,40°C, 1:2摩尔比- Lig-MNP/CALB达到90%的最大EO转化率。该固定化酶在多次循环中表现出操作稳定性,并且易于通过磁分离回收。纯化的生物燃料的油酸乙酯纯度达到约90%,总酯含量达到98%。这些结果突出了生物催化剂在可持续生物燃料生产方面的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Synthesis of biofuel esters catalyzed by lipase B from Candida antarctica immobilized on a magnetic lignin-based support derived from cashew apple bagasse

Synthesis of biofuel esters catalyzed by lipase B from Candida antarctica immobilized on a magnetic lignin-based support derived from cashew apple bagasse

This study investigated the biosynthesis of ethyl oleate (EO), the main constituent of biodiesel, via the esterification of oleic acid with ethanol, catalyzed by Candida antarctica lipase B (CALB) immobilized on a low-cost support derived from cashew apple bagasse (CAB) lignin. Cashew apple bagasse lignin extraction was carried out through an acid-alkali process and used to produce the CAB-lignin conjugated with magnetite (Lig-MNP) composite, which served as the support for enzyme immobilization. The biocatalyst (Lig-MNP/CALB), obtained through the immobilization process, exhibited an activity of 13 U g−1 and was subsequently applied in esterification reactions to evaluate its catalytic efficiency in comparison to the free enzyme. Acid-to-alcohol molar ratio, temperature, and catalytic load were evaluated. Under optimal conditions – 40 mg mL−1 catalyst load, 40 °C, and a 1:2 molar ratio – Lig-MNP/CALB achieved a maximum EO conversion of 90%. The immobilized enzyme demonstrated operational stability over multiple cycles and was easily recovered through magnetic separation. The purified biofuel reached approximately 90% purity in ethyl oleate and 98% in total esters content. These results highlight the potential of the biocatalyst for sustainable biofuel production.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
7.80
自引率
5.10%
发文量
122
审稿时长
4.5 months
期刊介绍: Biofuels, Bioproducts and Biorefining is a vital source of information on sustainable products, fuels and energy. Examining the spectrum of international scientific research and industrial development along the entire supply chain, The journal publishes a balanced mixture of peer-reviewed critical reviews, commentary, business news highlights, policy updates and patent intelligence. Biofuels, Bioproducts and Biorefining is dedicated to fostering growth in the biorenewables sector and serving its growing interdisciplinary community by providing a unique, systems-based insight into technologies in these fields as well as their industrial development.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信