界面硅化有效驱动间充质干细胞成骨分化而不需要任何外源性骨诱导因子。

IF 15.8 1区 材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY
ACS Nano Pub Date : 2025-07-17 DOI:10.1021/acsnano.5c01628
Zhouping Tian,Jiangfan Cao,Hailin Li,Junxian Yang,Jiangfeng Mao,Quhuan Li,Qi Lei,Honglin Chen,Wei Zhu
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引用次数: 0

摘要

间充质干细胞(MSCs)是一种多能成体干细胞,能够分化为各种细胞类型,包括成骨细胞。有效地引导MSC分化对再生医学至关重要,但目前的策略往往依赖于复杂的介质依赖系统。在这里,我们介绍了一种创新的技术─界面硅化,它可以有效地诱导MSC成骨分化,而不需要外源性骨诱导因子,如骨形态发生蛋白-2 (BMP-2)和地塞米松。这种方法利用两亲性肽促进二氧化硅前体在细胞界面的积累和凝聚。肽的疏水部分将其锚定在细胞膜上,而亲水的RRIL序列驱动可控的二氧化硅沉积。组学分析显示,界面硅化显著改变间充质干细胞细胞外基质,增强骨钙素和I型胶原等骨基质蛋白的生成,上调bmp和RUNX2等关键成骨因子,从而诱导小鼠和人间充质干细胞的强大成骨分化。值得注意的是,硅化MSCs表现出碱性磷酸酶活性增加,骨基质蛋白表达增强,矿化改善。总之,这种简单的、不依赖于培养基的方法为指导再生医学中的MSCs分化开辟了一条新的途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Interfacial Silicification Efficiently Drives Osteogenic Differentiation of Mesenchymal Stem Cells without Any Exogenous Osteoinductive Factor.
Mesenchymal stem cells (MSCs) are pluripotent adult stem cells capable of differentiating into various cell types, including osteoblasts. Efficiently directing MSC differentiation is crucial for regenerative medicine, but current strategies often rely on complex, media-dependent systems. Here, we introduce an innovative technology─interfacial silicification, which efficiently induces MSC osteogenic differentiation without the need for exogenous osteoinductive factors such as bone morphogenetic proteins-2 (BMP-2) and dexamethasone. This approach utilizes an amphiphilic peptide to facilitate the accumulation and condensation of silica precursors at the cell interface. The hydrophobic segment of the peptide anchors it to the cell membrane, while the hydrophilic RRIL sequence drives controlled silica deposition. Omic analyses revealed that interfacial silicification significantly alters the MSC extracellular matrix, enhances the production of bone matrix proteins such as osteocalcin and collagen type I, and upregulates key osteogenic factors, including BMPs and RUNX2, thereby inducing robust osteogenic differentiation in both mouse and human MSCs. Notably, silicified MSCs exhibited increased alkaline phosphatase activity, enhanced expression of bone matrix proteins, and improved mineralization. Together, this simple, media-independent method opens a new avenue for directing MSCs differentiation in regenerative medicine.
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来源期刊
ACS Nano
ACS Nano 工程技术-材料科学:综合
CiteScore
26.00
自引率
4.10%
发文量
1627
审稿时长
1.7 months
期刊介绍: ACS Nano, published monthly, serves as an international forum for comprehensive articles on nanoscience and nanotechnology research at the intersections of chemistry, biology, materials science, physics, and engineering. The journal fosters communication among scientists in these communities, facilitating collaboration, new research opportunities, and advancements through discoveries. ACS Nano covers synthesis, assembly, characterization, theory, and simulation of nanostructures, nanobiotechnology, nanofabrication, methods and tools for nanoscience and nanotechnology, and self- and directed-assembly. Alongside original research articles, it offers thorough reviews, perspectives on cutting-edge research, and discussions envisioning the future of nanoscience and nanotechnology.
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