靶向质粒偶联肉桂酸:对抗抗生素耐药性的新途径

IF 10.1 1区 工程技术 Q1 ENGINEERING, MULTIDISCIPLINARY
Gong Li, Ang Gao, Xin-Yi Lu, Tian-Hong Zhou, Shi-Ying Zhou, Li-Jua n Xia, Lei Wan, Yu-Zhang He, Xin-Yi Chen, Wen-Ying Guo, Jia-Min Zheng, Hao Ren, Sheng-Qiu Tang, Xiao-Ping Liao, Liang Chen, Jian Sun
{"title":"靶向质粒偶联肉桂酸:对抗抗生素耐药性的新途径","authors":"Gong Li, Ang Gao, Xin-Yi Lu, Tian-Hong Zhou, Shi-Ying Zhou, Li-Jua n Xia, Lei Wan, Yu-Zhang He, Xin-Yi Chen, Wen-Ying Guo, Jia-Min Zheng, Hao Ren, Sheng-Qiu Tang, Xiao-Ping Liao, Liang Chen, Jian Sun","doi":"10.1016/j.eng.2025.06.040","DOIUrl":null,"url":null,"abstract":"The global spread of antibiotic resistance genes (ARGs) continues to worsen, with plasmid-mediated conjugation serving as a major transmission route. Although developing conjugation inhibitors to block this process is a promising strategy, current options are limited by toxicity and poor <em>in vivo</em> efficacy. This study evaluated the effect of cinnamic acid (CA; 3-phenyl-2-acrylic acid), a widely abundant food additive found in cinnamon, on plasmid conjugation. CA effectively inhibited the conjugation of various resistance plasmids <em>in vitro</em>, <em>ex vivo</em>, and <em>in vivo</em>. Transcriptomic analysis indicated that CA disrupts the electron transport chain (ETC) and proton motive force (PMF) by inhibiting the tricarboxylic acid (TCA) cycle, leading to reduced intracellular adenosine triphosphate (ATP)—a critical factor for plasmid conjugation. Biocompatibility assays showed that CA maintains high biosafety while preserving gut microbiota homeostasis. Therefore, these findings provide new insights into ARG inhibition and highlight the potential of CA as a novel strategy to combat the global rise in antibiotic-resistant infections.","PeriodicalId":11783,"journal":{"name":"Engineering","volume":"16 1","pages":""},"PeriodicalIF":10.1000,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Targeting Plasmid Conjugation with Cinnamic Acid: A Novel Approach to Combat Antibiotic Resistance\",\"authors\":\"Gong Li, Ang Gao, Xin-Yi Lu, Tian-Hong Zhou, Shi-Ying Zhou, Li-Jua n Xia, Lei Wan, Yu-Zhang He, Xin-Yi Chen, Wen-Ying Guo, Jia-Min Zheng, Hao Ren, Sheng-Qiu Tang, Xiao-Ping Liao, Liang Chen, Jian Sun\",\"doi\":\"10.1016/j.eng.2025.06.040\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The global spread of antibiotic resistance genes (ARGs) continues to worsen, with plasmid-mediated conjugation serving as a major transmission route. Although developing conjugation inhibitors to block this process is a promising strategy, current options are limited by toxicity and poor <em>in vivo</em> efficacy. This study evaluated the effect of cinnamic acid (CA; 3-phenyl-2-acrylic acid), a widely abundant food additive found in cinnamon, on plasmid conjugation. CA effectively inhibited the conjugation of various resistance plasmids <em>in vitro</em>, <em>ex vivo</em>, and <em>in vivo</em>. Transcriptomic analysis indicated that CA disrupts the electron transport chain (ETC) and proton motive force (PMF) by inhibiting the tricarboxylic acid (TCA) cycle, leading to reduced intracellular adenosine triphosphate (ATP)—a critical factor for plasmid conjugation. Biocompatibility assays showed that CA maintains high biosafety while preserving gut microbiota homeostasis. Therefore, these findings provide new insights into ARG inhibition and highlight the potential of CA as a novel strategy to combat the global rise in antibiotic-resistant infections.\",\"PeriodicalId\":11783,\"journal\":{\"name\":\"Engineering\",\"volume\":\"16 1\",\"pages\":\"\"},\"PeriodicalIF\":10.1000,\"publicationDate\":\"2025-07-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Engineering\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1016/j.eng.2025.06.040\",\"RegionNum\":1,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Engineering","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1016/j.eng.2025.06.040","RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0

摘要

抗生素耐药基因(ARGs)的全球传播继续恶化,质粒介导的结合是主要的传播途径。虽然开发偶联抑制剂来阻断这一过程是一个很有前途的策略,但目前的选择受到毒性和体内疗效差的限制。本研究评价了肉桂酸(CA;肉桂中广泛存在的食品添加剂3-苯基-2-丙烯酸(3-苯基-2-丙烯酸)。CA在体外、离体和体内均能有效抑制多种抗性质粒的结合。转录组学分析表明,CA通过抑制三羧酸(TCA)循环破坏电子传递链(ETC)和质子动力(PMF),导致细胞内三磷酸腺苷(ATP)减少,ATP是质粒结合的关键因素。生物相容性试验表明,CA在保持肠道菌群稳态的同时保持了较高的生物安全性。因此,这些发现为ARG抑制提供了新的见解,并突出了CA作为对抗全球抗生素耐药感染上升的新策略的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Targeting Plasmid Conjugation with Cinnamic Acid: A Novel Approach to Combat Antibiotic Resistance

Targeting Plasmid Conjugation with Cinnamic Acid: A Novel Approach to Combat Antibiotic Resistance
The global spread of antibiotic resistance genes (ARGs) continues to worsen, with plasmid-mediated conjugation serving as a major transmission route. Although developing conjugation inhibitors to block this process is a promising strategy, current options are limited by toxicity and poor in vivo efficacy. This study evaluated the effect of cinnamic acid (CA; 3-phenyl-2-acrylic acid), a widely abundant food additive found in cinnamon, on plasmid conjugation. CA effectively inhibited the conjugation of various resistance plasmids in vitro, ex vivo, and in vivo. Transcriptomic analysis indicated that CA disrupts the electron transport chain (ETC) and proton motive force (PMF) by inhibiting the tricarboxylic acid (TCA) cycle, leading to reduced intracellular adenosine triphosphate (ATP)—a critical factor for plasmid conjugation. Biocompatibility assays showed that CA maintains high biosafety while preserving gut microbiota homeostasis. Therefore, these findings provide new insights into ARG inhibition and highlight the potential of CA as a novel strategy to combat the global rise in antibiotic-resistant infections.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Engineering
Engineering Environmental Science-Environmental Engineering
自引率
1.60%
发文量
335
审稿时长
35 days
期刊介绍: Engineering, an international open-access journal initiated by the Chinese Academy of Engineering (CAE) in 2015, serves as a distinguished platform for disseminating cutting-edge advancements in engineering R&D, sharing major research outputs, and highlighting key achievements worldwide. The journal's objectives encompass reporting progress in engineering science, fostering discussions on hot topics, addressing areas of interest, challenges, and prospects in engineering development, while considering human and environmental well-being and ethics in engineering. It aims to inspire breakthroughs and innovations with profound economic and social significance, propelling them to advanced international standards and transforming them into a new productive force. Ultimately, this endeavor seeks to bring about positive changes globally, benefit humanity, and shape a new future.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信