黄芩黄芩素抑制脓肿分枝杆菌生物膜形成，恢复抗生素敏感性。

IF 2 3区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biofouling Pub Date : 2025-08-01 Epub Date: 2025-07-15 DOI:10.1080/08927014.2025.2531139

Xiaorui Zhang, Baoyu Dong, Qiurong He, Xingyan Tan, Yuqing Li, Kelei Zhao, Xinyue Xu, Jumei Zeng

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引用次数: 0

摘要

脓肿分枝杆菌对传统的抗结核药物和目前大多数可用的抗生素具有耐药性，其强大的生物膜形成可能有助于耐药并使情况更加恶化。在本研究中，黄芩提取物显著抑制了脓肿支原体浮游生物的生长和生物膜的形成。黄芩苷是黄芩提取物的主要活性成分，它抑制了脓肿支原体生物膜的形成，而不是浮游生物的生长，并降低了脓肿支原体生物膜的细胞外基质脂质。研究黄芩苷与临床药物联合使用的协同作用，黄芩苷能有效恢复脓肿分枝杆菌对所研究药物在生物膜生长中的敏感性。转录组分析显示，黄芩素处理后，98个基因表达上调，19个基因表达下调，RT-qPCR验证了脂肪酸合成通路相关基因。因此，黄芩提取物及其单体黄芩苷有可能作为预防和治疗脓肿分枝杆菌慢性感染生物膜形成的新型佐剂。

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Baicalein of Scutellaria baicalensis inhibits Mycobacterium abscessus biofilm formation to restore the antibiotics susceptibility.

Mycobacterium abscessus is resistant to traditional anti-TB medications and most currently available antibiotics, its strong biofilm formation may contribute to the resistance and make the situation even worse. In this study, Scutellaria baicalensis extract significantly inhibited the planktonic growth as well as the biofilm formation of M. abscessus. Baicalein, the principal active element in S. baicalensis extract, inhibited the biofilm formation instead of the planktonic growth and reduced extracellular matrix lipids in M. abscessus biofilm. The synergistic effects of baicalein in combination with clinical drugs were investigated, the baicalein effectively restored the susceptibility of M. abscessus to the investigated drugs in the biofilm growth. The transcriptome analysis revealed that 98 genes were upregulated and 19 genes were downregulated after baicalein treatment, genes involved in the fatty acid synthesis pathway were validated by RT-qPCR. Therefore, the extract of S. baicalensis and its monomer baicalein might serve as potential innovative adjuvant agents for the prevention and treatment of M. abscessus biofilm formation in chronic infections.

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来源期刊

Biofouling 生物-海洋与淡水生物学

CiteScore

5.00

自引率

7.40%

发文量

审稿时长

1.7 months

期刊介绍： Biofouling is an international, peer-reviewed, multi-discliplinary journal which publishes original articles and mini-reviews and provides a forum for publication of pure and applied work on protein, microbial, fungal, plant and animal fouling and its control, as well as studies of all kinds on biofilms and bioadhesion. Papers may be based on studies relating to characterisation, attachment, growth and control on any natural (living) or man-made surface in the freshwater, marine or aerial environments, including fouling, biofilms and bioadhesion in the medical, dental, and industrial context. Specific areas of interest include antifouling technologies and coatings including transmission of invasive species, antimicrobial agents, biological interfaces, biomaterials, microbiologically influenced corrosion, membrane biofouling, food industry biofilms, biofilm based diseases and indwelling biomedical devices as substrata for fouling and biofilm growth, including papers based on clinically-relevant work using models that mimic the realistic environment in which they are intended to be used.