[二硒烯交联纳米虾青素对庆大霉素致小鼠前庭毒性的保护作用及其机制]。

Q3 Medicine
Z Y Wang, S Q Yang, S X Wang, X Chen, M Z Cheng, Y F Yu
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引用次数: 0

摘要

目的:探讨双硒交联虾青素纳米载体(AST@dSe-AFT)鼓室内给药对庆大霉素(GM)致小鼠前庭毒性的保护作用及其机制。方法:选取6 ~ 8周龄雄性ICR小鼠42只,按随机数字表法随机分为7组:对照组、GM损伤组、天然虾青素(AST)保护组(GM+AST组)和4个AST@dse-AFT保护组(GM+AST/NP 0.1组、GM+AST/NP 1组、GM+AST/NP 10组、GM+AST/NP 100组),分别给予0.1、1、10、100 mg/ml AST@dSe-AFT。对照组不进行任何干预,GM损伤组通过连续7天腹腔注射200 mg/kg GM构建前庭损伤模型;GM+AST组鼓室内注射1 mg/ml AST二甲亚砜溶液0.1 ml,其余4组鼓室内注射相应浓度的AST@dSe-AFT。然后建立前庭损伤模型。通过游泳实验、平衡木实验和旋转杆疲劳仪实验评估前庭功能。免疫荧光染色观察小室、小囊黄斑毛细胞的形态和数量,分析前庭组织中凋亡相关蛋白的表达水平。结果:行为学实验显示,GM+AST/NP 10组和GM+AST/NP 100组的平衡木通过时间均短于GM损伤组[(18.8±1.5)和(19.3±1.2)vs(33.9±2.0)s,均为ppppp。结论:鼓室内注射AST@dSe-AFT可拮抗GM诱导的前庭损伤,其对前庭功能的保护作用可能与抑制毛细胞凋亡有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Protective effect and its mechanism of diselenide cross-linked nano-loaded astaxanthin on gentamicin-induced vestibular toxicity in mice].

Objective: To investigate the protective effect and its mechanism of intratympanic administration of diselenide cross-linked nano-carriers of astaxanthin (AST@dSe-AFT) on vestibular toxicity induced by gentamicin (GM) in mice. Methods: Forty-two male ICR mice aged 6-8 weeks were randomly divided into seven groups according to a random number table: control group, GM injury group, natural astaxanthin (AST) protection group (GM+AST group), and four AST@dse-AFT protection groups (GM+AST/NP 0.1 group, GM+AST/NP 1 group, GM+AST/NP 10 group, and GM+AST/NP 100 group), which were given 0.1, 1, 10, and 100 mg/ml AST@dSe-AFT, respectively. The control group did not receive any intervention;the GM injury group was established by intraperitoneal injection of 200 mg/kg GM for 7 consecutive days to construct a vestibular injury model; the GM+AST group received an intratympanic injection of 0.1 ml of 1 mg/ml AST dimethyl sulfoxide solution, while the remaining four groups received intratympanic injections of corresponding concentrations of AST@dSe-AFT. Afterwards, a vestibular injury model was constructed. The vestibular function was evaluated through swimming experiments, balance beam experiments, and rotating rod fatigue instrument experiments. The morphology and number of hair cells in the macula of the utricle and saccule were observed by immunofluorescence staining, and the expression levels of apoptosis-related proteins in the vestibular tissues were analyzed. Results: The behavioral experiments revealed that the balance beam passing time in the GM+AST/NP 10 and GM+AST/NP 100 groups were shorter than that in the GM injury group [(18.8±1.5) and (19.3±1.2) vs (33.9±2.0) s, all P<0.05], and the total distance,dwell time and speed of the rotating rod fatigue test were higher than those in the GM injury group [(2.9±0.4), (3.3±0.6), (1.0±0.1) m; (121.6±9.2), (125.7±11.4), (70.9±5.1) s; (30.7±2.4), (31.7±3.0), (17.2±1.4) m/s, respectively, all P<0.05]. Immunofluorescence staining results showed that the number of hair cells in the utricle macula in the GM+AST/NP 10 and GM+AST/NP 100 groups were higher than that in the GM injury group (all P<0.05). The expression of apoptosis-related proteins Cleaved caspase 3, Cleaved caspase 9, and Cleaved PARP-1 in the GM+AST/NP 10 group was lower than that in the GM injury group (all P<0.05). Conclusions: Intratympanic injection of AST@dSe-AFT can antagonize GM-induced vestibular damage, and its protective effect on vestibular function may be related to the inhibition of hair cell apoptosis.

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来源期刊
Zhonghua yi xue za zhi
Zhonghua yi xue za zhi Medicine-Medicine (all)
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