严重神经损伤儿童的细胞因子失调与显著的临床结果相关。

IF 2.1 3区 医学 Q2 PEDIATRICS
Frontiers in Pediatrics Pub Date : 2025-06-30 eCollection Date: 2025-01-01 DOI:10.3389/fped.2025.1567221
John Allen, Johana Isaza-Correa, Lynne Kelly, Ashanty Melo, Conor Power, Aoife Mahony, Denise McDonald, Eleanor J Molloy
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引用次数: 0

摘要

背景:患有神经系统疾病的儿童炎症反应发生改变。我们的目的是描述血清中促炎、抗炎和缺氧诱导的细胞因子,基线时,以及对全血脂多糖刺激的反应,在患有严重神经损伤(SNI)的儿童中与对照组相比。方法:将SNI患儿和健康对照者的全血样本在存在或不存在脂多糖(LPS)的条件下孵育。分离血清,ELISA分析12种细胞因子。从医疗记录中收集精选临床数据,并与细胞因子结果相关联。结果:招募了29名SNI患儿(n = 14)和年龄匹配的对照组(n = 15)。干扰素(INF)-γ、白细胞介素(IL)-18、肿瘤坏死因子(TNF)-β、IL-10、IL-1ra、IL-1β、IL-8、TNF-α和血管内皮生长因子(VEGF)对脂多糖的反应在两组之间相似。对照组粒细胞单核细胞集落刺激因子(GM-CSF)对LPS的反应增加(p = 0.04), SNI组则无升高(p = 0.07)。SNI组在LPS作用下EPO显著高于对照组(p = 0.006)。SNI组IL-6对LPS的反应相对较低(p = 0.01)。抗癫痫药物剂量与IL-1ra (p = 0.01)、TNF-α (p = 0.04)相关;最近一年内感染人数与IL-18 (p = 0.02);肠内喂养、IL-10 (p = 0.03)和EPO需求(p = 0.001);预防性抗生素和IL-10的使用(p = 0.001);呼吸支持和VEGF需求(p = 0.007)。结论:SNI患儿具有持续性炎症反应改变。这些改变可能导致三级神经损伤和对感染的反应能力受损,并可能为免疫调节提供靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cytokine dysregulation in children with severe neurological impairment correlates with significant clinical outcomes.

Background: Children with neurological disorders have altered inflammatory responses. We aimed to describe pro-inflammatory, anti-inflammatory and hypoxia-induced cytokines in serum, at baseline, and in response to stimulation of whole blood with lipopolysaccharide, in children with Severe Neurological Impairment (SNI) compared to controls.

Methods: Whole blood samples from children with SNI and healthy controls were incubated in the presence or absence of lipopolysaccharide (LPS). Serum was isolated and 12 cytokines were analysed by ELISA. Select clinical data was collected from healthcare records and correlated with cytokine results.

Results: Twenty-nine children with SNI (n = 14) and age-matched controls (n = 15) were recruited. Cytokine responses to lipopolysaccharide were similar between the groups for Interferon (INF)-γ, Interleukin(IL)-18, Tumour Necrosis Factor(TNF)-β, IL-10, IL-1ra, IL-1β, IL-8, TNF-α and Vascular Endothelial Growth Factor (VEGF). Granulocyte Monocyte Colony Stimulating Factor (GM-CSF) increased in response to LPS in the control group (p = 0.04) but not in those with SNI (p = 0.07). The SNI cohort had a significantly greater increase in EPO in response to LPS than controls (p = 0.006). IL-6 in the SNI cohort was relatively hyporesponsive to LPS (p = 0.01). Correlations were found in LPS responses as follows: number of antiseizure medications and IL-1ra (p = 0.01) and TNF-α (p = 0.04); number of infections within the last year and IL-18 (p = 0.02); requirement for enteral feeding and IL-10 (p = 0.03) and EPO (p = 0.001); use of prophylactic antibiotics and IL-10 (p = 0.001); requirement for respiratory support and VEGF (p = 0.007).

Conclusion: Children with SNI have persistent altered inflammatory responses. These alterations may contribute to tertiary neurological injury and impaired ability to respond to infection and may provide a target for immunomodulation.

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来源期刊
Frontiers in Pediatrics
Frontiers in Pediatrics Medicine-Pediatrics, Perinatology and Child Health
CiteScore
3.60
自引率
7.70%
发文量
2132
审稿时长
14 weeks
期刊介绍: Frontiers in Pediatrics (Impact Factor 2.33) publishes rigorously peer-reviewed research broadly across the field, from basic to clinical research that meets ongoing challenges in pediatric patient care and child health. Field Chief Editors Arjan Te Pas at Leiden University and Michael L. Moritz at the Children''s Hospital of Pittsburgh are supported by an outstanding Editorial Board of international experts. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide. Frontiers in Pediatrics also features Research Topics, Frontiers special theme-focused issues managed by Guest Associate Editors, addressing important areas in pediatrics. In this fashion, Frontiers serves as an outlet to publish the broadest aspects of pediatrics in both basic and clinical research, including high-quality reviews, case reports, editorials and commentaries related to all aspects of pediatrics.
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