猪粪的微生物分析:对生物肥料应用的影响。

IF 1.9 4区 生物学 Q3 MICROBIOLOGY
Brazilian Journal of Microbiology Pub Date : 2025-09-01 Epub Date: 2025-07-15 DOI:10.1007/s42770-025-01737-3
João Victor Pessoa Maia, Franciele Natividade Luiz, Marahia Isabel Guevara Robayo, Deborah Catharine de Assis Leite, Rafaela Faust Meyer, Michel Rodrigo Zambrano Passarini
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引用次数: 0

摘要

由于石油基产品造成的严重污染,寻找可持续能源变得更加紧迫。消化物是生物甲烷生产过程中产生的有机废物消化的副产品,可以用作生物肥料。这种化合物丰富土壤,促进固氮细菌的繁殖,有利于可持续农业。本研究评估了两个厌氧消化器(中试和实验室规模)利用猪尸体和污水产生的消化液的生物肥料潜力。理化分析和微生物多样性谱揭示了消化系统之间的差异。在实验室规模的沼气池中,微生物多样性更高,这表明猪尸体的存在有利于形成更多样化的群落,这对生产生物肥料至关重要。实验室规模和中试规模的Shannon多样性指数分别为7.6和5.4。实验室规模沼气池中最丰富的菌属为甲烷菌(25.38%)、梭菌(7.08%)和假单胞菌(5.65%)。另一方面,在中试规模上,丰度最高的是Clostridium(20.90%)、Turicibacter(14.13%)和Methanosarcina(3.78%)。在两个尺度上均发现了芽孢杆菌、假单胞菌和缓生根瘤菌等有益菌属,它们可能具有促进植物生长、增磷和固氮的作用。实验室规模的甲烷菌丰度较高。结果表明,实验室规模消化池的消化液具有更大的微生物多样性和更高的有益类群存在,包括芽孢杆菌(2.2%)和芽孢杆菌(1.5%),已知通过植物激素的产生刺激植物生长。然而,需要进一步的研究,包括代谢途径或现场试验,以了解与猪粪便(包括动物尸体)消化物相关的微生物群的变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Microbial profiling of swine-waste digestates: implications for biofertilizer application.

The search for sustainable energy sources has become more urgent due to the high level of pollution caused by petroleum-based products. Digestate, a byproduct of the digestion of organic waste generated during biomethane production, can be used as a biofertilizer. This compound enriches the soil and promotes the proliferation of nitrogen-fixing bacteria, which benefits sustainable agriculture. This study assessed the biofertilizer potential of digestates from two anaerobic digesters (pilot and laboratory scale) using swine carcasses and effluent. Physicochemical analyses and microbial diversity profiles revealed differences between the digestates. The microbial diversity was higher in the laboratory-scale digester, suggesting that the presence of swine carcasses favored the development of a more diverse community, which is essential for producing a biofertilizer. Shannon diversity indices for laboratory scale and pilot scale were 7.6 and 5.4, respectively. The most abundant genera observed in the laboratory-scale digester were Methanoculleus (25.38%), Clostridium (7.08%), and Pseudomonas (5.65%). On the other hand, at pilot scale, the greatest abundance was associated with Clostridium (20.90%), Turicibacter (14.13%), and Methanosarcina (3.78%). Beneficial genera including Bacillus, Pseudomonas and Bradyrhizobium were found in both scales, which may play roles in plant growth promotion, phosphorus solubilization, and nitrogen fixation. The abundance of Methanoculleus was higher at the laboratory scale. The results indicate that digestate from the laboratory-scale digester had a greater microbial diversity and a higher presence of beneficial taxa, including Bacillus (2.2%) and Paenibacillus (1.5%), known to stimulate plant growth through phytohormone production. However, further studies including metabolic pathways or field testing are needed to understand the variation in microbiota associated with digestates from swine waste, including animal carcasses.

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来源期刊
Brazilian Journal of Microbiology
Brazilian Journal of Microbiology 生物-微生物学
CiteScore
4.10
自引率
4.50%
发文量
216
审稿时长
1.0 months
期刊介绍: The Brazilian Journal of Microbiology is an international peer reviewed journal that covers a wide-range of research on fundamental and applied aspects of microbiology. The journal considers for publication original research articles, short communications, reviews, and letters to the editor, that may be submitted to the following sections: Biotechnology and Industrial Microbiology, Food Microbiology, Bacterial and Fungal Pathogenesis, Clinical Microbiology, Environmental Microbiology, Veterinary Microbiology, Fungal and Bacterial Physiology, Bacterial, Fungal and Virus Molecular Biology, Education in Microbiology. For more details on each section, please check out the instructions for authors. The journal is the official publication of the Brazilian Society of Microbiology and currently publishes 4 issues per year.
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