{"title":"透析过程中使用创新的直接溶解技术的高浓度氢输送:犬模型的体内动力学。","authors":"Masaki Shibuya, Masafumi Fujinaka, Mako Yonezawa, Natsumi Nishimura, Hitoshi Uchinoumi, Kenji Tani, Yukihiro Hitaka, Kimihiko Nakamura, Naohito Isoyama, Zenzo Fujii, Motoaki Sano","doi":"10.1097/MAT.0000000000002508","DOIUrl":null,"url":null,"abstract":"<p><p>Hydrogen gas (H₂) shows broad therapeutic potential. Hemodialysis, using large dialysate volumes in contact with blood, presents a promising H₂ delivery method. We developed an innovative system generating hydrogen-enriched dialysate, differing from conventional electrolysis. This system directly dissolves H₂ gas into tap water to produce saturated water, which then undergoes reverse osmosis (RO) for dialysate preparation. Using this system in a canine hemodialysis model with a single dog, we measured H₂ concentrations. High H₂ levels were consistently maintained (approximately 1,600 ppb in RO water; stable approximately 230 ppb in final dialysate). H₂ efficiently diffused into the extracorporeal blood circuit, with outlet concentrations reaching 54.0-67.7% of the dialysate level. However, low systemic arterial concentrations (pulmonary, carotid) indicated significant pulmonary clearance, suggesting H₂ primarily acts locally within the circuit and dialyzer. Compared with traditional electrolyzed water methods, this direct dissolution system delivers substantially higher and more stable H₂ concentrations. Its simpler design and potentially lower installation costs suggest feasibility for widespread clinical adoption. Future studies should explore hemodiafiltration (HDF) to potentially enhance systemic H₂ delivery and evaluate long-term clinical benefits.</p>","PeriodicalId":8844,"journal":{"name":"ASAIO Journal","volume":" ","pages":""},"PeriodicalIF":3.1000,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"High-Concentration Hydrogen Delivery During Dialysis Using an Innovative Direct Dissolution Technique: In Vivo Kinetics in a Canine Model.\",\"authors\":\"Masaki Shibuya, Masafumi Fujinaka, Mako Yonezawa, Natsumi Nishimura, Hitoshi Uchinoumi, Kenji Tani, Yukihiro Hitaka, Kimihiko Nakamura, Naohito Isoyama, Zenzo Fujii, Motoaki Sano\",\"doi\":\"10.1097/MAT.0000000000002508\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Hydrogen gas (H₂) shows broad therapeutic potential. Hemodialysis, using large dialysate volumes in contact with blood, presents a promising H₂ delivery method. We developed an innovative system generating hydrogen-enriched dialysate, differing from conventional electrolysis. This system directly dissolves H₂ gas into tap water to produce saturated water, which then undergoes reverse osmosis (RO) for dialysate preparation. Using this system in a canine hemodialysis model with a single dog, we measured H₂ concentrations. High H₂ levels were consistently maintained (approximately 1,600 ppb in RO water; stable approximately 230 ppb in final dialysate). H₂ efficiently diffused into the extracorporeal blood circuit, with outlet concentrations reaching 54.0-67.7% of the dialysate level. However, low systemic arterial concentrations (pulmonary, carotid) indicated significant pulmonary clearance, suggesting H₂ primarily acts locally within the circuit and dialyzer. Compared with traditional electrolyzed water methods, this direct dissolution system delivers substantially higher and more stable H₂ concentrations. Its simpler design and potentially lower installation costs suggest feasibility for widespread clinical adoption. Future studies should explore hemodiafiltration (HDF) to potentially enhance systemic H₂ delivery and evaluate long-term clinical benefits.</p>\",\"PeriodicalId\":8844,\"journal\":{\"name\":\"ASAIO Journal\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.1000,\"publicationDate\":\"2025-07-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ASAIO Journal\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1097/MAT.0000000000002508\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENGINEERING, BIOMEDICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ASAIO Journal","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1097/MAT.0000000000002508","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
High-Concentration Hydrogen Delivery During Dialysis Using an Innovative Direct Dissolution Technique: In Vivo Kinetics in a Canine Model.
Hydrogen gas (H₂) shows broad therapeutic potential. Hemodialysis, using large dialysate volumes in contact with blood, presents a promising H₂ delivery method. We developed an innovative system generating hydrogen-enriched dialysate, differing from conventional electrolysis. This system directly dissolves H₂ gas into tap water to produce saturated water, which then undergoes reverse osmosis (RO) for dialysate preparation. Using this system in a canine hemodialysis model with a single dog, we measured H₂ concentrations. High H₂ levels were consistently maintained (approximately 1,600 ppb in RO water; stable approximately 230 ppb in final dialysate). H₂ efficiently diffused into the extracorporeal blood circuit, with outlet concentrations reaching 54.0-67.7% of the dialysate level. However, low systemic arterial concentrations (pulmonary, carotid) indicated significant pulmonary clearance, suggesting H₂ primarily acts locally within the circuit and dialyzer. Compared with traditional electrolyzed water methods, this direct dissolution system delivers substantially higher and more stable H₂ concentrations. Its simpler design and potentially lower installation costs suggest feasibility for widespread clinical adoption. Future studies should explore hemodiafiltration (HDF) to potentially enhance systemic H₂ delivery and evaluate long-term clinical benefits.
期刊介绍:
ASAIO Journal is in the forefront of artificial organ research and development. On the cutting edge of innovative technology, it features peer-reviewed articles of the highest quality that describe research, development, the most recent advances in the design of artificial organ devices and findings from initial testing. Bimonthly, the ASAIO Journal features state-of-the-art investigations, laboratory and clinical trials, and discussions and opinions from experts around the world.
The official publication of the American Society for Artificial Internal Organs.