Erica Almance, Suprina Shrestha, Ricardo Sánchez-Murillo, Brooke Best, Oscar Rojas-Carrillo, María Poca, Christian Birkel
{"title":"亚热带城市绿地木质部和土壤水分的离心提取","authors":"Erica Almance, Suprina Shrestha, Ricardo Sánchez-Murillo, Brooke Best, Oscar Rojas-Carrillo, María Poca, Christian Birkel","doi":"10.1002/eco.70068","DOIUrl":null,"url":null,"abstract":"<p>Extracting water from discrete xylem and soil samples for δ<sup>18</sup>O and δ<sup>2</sup>H analysis remains a central challenge in ecohydrology. This study evaluates the applicability of the centrifugation method in extracting (a) mobile water with (b) no induced fractionation and (c) low organic contamination. Three common urban tree species (elderberry, <i>Sambucus canadensis</i>; cherry laurel, <i>Prunus caroliniana</i>; and boxelder maple, <i>Acer negundo</i>) were studied for 1 year in the Fort Worth Botanic Garden (north-central Texas, United States). Xylem (<i>N</i> = 110) isotope values were compared alongside throughfall (<i>N</i> = 33), soil water (<i>N</i> = 79) and lysimeter water (<i>N</i> = 42) at different depths (0–38 cm). Xylem and soil water extraction volumes ranged from 0.1 to 7.5 mL and 0.1 to 10.5 mL, respectively. Extracted water volumes exhibited no strong correlations with isotopic values. Water was extracted from soil and xylem samples with gravimetric water contents as low as 10.6% and 17.8%, respectively. Although elderberry and cherry laurel exhibited narrowband and broadband values (indicators for organic contamination) similar to throughfall, soil water and lysimeter water, boxelder maple reported higher values in summer and fall. Annual δ<sup>18</sup>O mean values in soil water (−3.2‰ ± 1.7‰) and lysimeter water (−3.3‰ ± 1.1‰) corresponded with the throughfall input (−3.1‰ ± 2.0‰). Xylem δ<sup>18</sup>O exhibited higher values in winter, summer and fall than throughfall, soil water or lysimeter water. However, during spring, xylem mean δ<sup>18</sup>O values were less variable and close to soil and lysimeter δ<sup>18</sup>O values: elderberry (−3.4‰ ± 0.5‰), cherry laurel (2.9‰ ± 0.5‰) and boxelder (−2.3‰ ± 0.4‰). Our results highlight the applicability of the centrifugation method in extracting mobile water, unbiased by low extraction volumes, temperature-induced fractionation or high organic contamination. Low xylem and soil water contents limit the extraction capacity of centrifugation and increase the concentration of organics, particularly during summer and fall seasons.</p>","PeriodicalId":55169,"journal":{"name":"Ecohydrology","volume":"18 5","pages":""},"PeriodicalIF":2.1000,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/eco.70068","citationCount":"0","resultStr":"{\"title\":\"Xylem and Soil Water Extraction via Centrifugation in a Subtropical Urban Green Space\",\"authors\":\"Erica Almance, Suprina Shrestha, Ricardo Sánchez-Murillo, Brooke Best, Oscar Rojas-Carrillo, María Poca, Christian Birkel\",\"doi\":\"10.1002/eco.70068\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Extracting water from discrete xylem and soil samples for δ<sup>18</sup>O and δ<sup>2</sup>H analysis remains a central challenge in ecohydrology. This study evaluates the applicability of the centrifugation method in extracting (a) mobile water with (b) no induced fractionation and (c) low organic contamination. Three common urban tree species (elderberry, <i>Sambucus canadensis</i>; cherry laurel, <i>Prunus caroliniana</i>; and boxelder maple, <i>Acer negundo</i>) were studied for 1 year in the Fort Worth Botanic Garden (north-central Texas, United States). Xylem (<i>N</i> = 110) isotope values were compared alongside throughfall (<i>N</i> = 33), soil water (<i>N</i> = 79) and lysimeter water (<i>N</i> = 42) at different depths (0–38 cm). Xylem and soil water extraction volumes ranged from 0.1 to 7.5 mL and 0.1 to 10.5 mL, respectively. Extracted water volumes exhibited no strong correlations with isotopic values. Water was extracted from soil and xylem samples with gravimetric water contents as low as 10.6% and 17.8%, respectively. Although elderberry and cherry laurel exhibited narrowband and broadband values (indicators for organic contamination) similar to throughfall, soil water and lysimeter water, boxelder maple reported higher values in summer and fall. Annual δ<sup>18</sup>O mean values in soil water (−3.2‰ ± 1.7‰) and lysimeter water (−3.3‰ ± 1.1‰) corresponded with the throughfall input (−3.1‰ ± 2.0‰). Xylem δ<sup>18</sup>O exhibited higher values in winter, summer and fall than throughfall, soil water or lysimeter water. However, during spring, xylem mean δ<sup>18</sup>O values were less variable and close to soil and lysimeter δ<sup>18</sup>O values: elderberry (−3.4‰ ± 0.5‰), cherry laurel (2.9‰ ± 0.5‰) and boxelder (−2.3‰ ± 0.4‰). Our results highlight the applicability of the centrifugation method in extracting mobile water, unbiased by low extraction volumes, temperature-induced fractionation or high organic contamination. 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Xylem and Soil Water Extraction via Centrifugation in a Subtropical Urban Green Space
Extracting water from discrete xylem and soil samples for δ18O and δ2H analysis remains a central challenge in ecohydrology. This study evaluates the applicability of the centrifugation method in extracting (a) mobile water with (b) no induced fractionation and (c) low organic contamination. Three common urban tree species (elderberry, Sambucus canadensis; cherry laurel, Prunus caroliniana; and boxelder maple, Acer negundo) were studied for 1 year in the Fort Worth Botanic Garden (north-central Texas, United States). Xylem (N = 110) isotope values were compared alongside throughfall (N = 33), soil water (N = 79) and lysimeter water (N = 42) at different depths (0–38 cm). Xylem and soil water extraction volumes ranged from 0.1 to 7.5 mL and 0.1 to 10.5 mL, respectively. Extracted water volumes exhibited no strong correlations with isotopic values. Water was extracted from soil and xylem samples with gravimetric water contents as low as 10.6% and 17.8%, respectively. Although elderberry and cherry laurel exhibited narrowband and broadband values (indicators for organic contamination) similar to throughfall, soil water and lysimeter water, boxelder maple reported higher values in summer and fall. Annual δ18O mean values in soil water (−3.2‰ ± 1.7‰) and lysimeter water (−3.3‰ ± 1.1‰) corresponded with the throughfall input (−3.1‰ ± 2.0‰). Xylem δ18O exhibited higher values in winter, summer and fall than throughfall, soil water or lysimeter water. However, during spring, xylem mean δ18O values were less variable and close to soil and lysimeter δ18O values: elderberry (−3.4‰ ± 0.5‰), cherry laurel (2.9‰ ± 0.5‰) and boxelder (−2.3‰ ± 0.4‰). Our results highlight the applicability of the centrifugation method in extracting mobile water, unbiased by low extraction volumes, temperature-induced fractionation or high organic contamination. Low xylem and soil water contents limit the extraction capacity of centrifugation and increase the concentration of organics, particularly during summer and fall seasons.
期刊介绍：
Ecohydrology is an international journal publishing original scientific and review papers that aim to improve understanding of processes at the interface between ecology and hydrology and associated applications related to environmental management.
Ecohydrology seeks to increase interdisciplinary insights by placing particular emphasis on interactions and associated feedbacks in both space and time between ecological systems and the hydrological cycle. Research contributions are solicited from disciplines focusing on the physical, ecological, biological, biogeochemical, geomorphological, drainage basin, mathematical and methodological aspects of ecohydrology. Research in both terrestrial and aquatic systems is of interest provided it explicitly links ecological systems and the hydrologic cycle; research such as aquatic ecological, channel engineering, or ecological or hydrological modelling is less appropriate for the journal unless it specifically addresses the criteria above. Manuscripts describing individual case studies are of interest in cases where broader insights are discussed beyond site- and species-specific results.
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