改良的牛链球菌MALDI-TOF质谱文库的建立与验证。适应诊断培养和提取条件的马科复合体亚种鉴定

IF 2.1 4区医学 Q3 INFECTIOUS DISEASES

Diagnostic microbiology and infectious disease Pub Date : 2025-07-10 DOI:10.1016/j.diagmicrobio.2025.117000

Jonas Öberg , Agnes Engberg , Malin Inghammar , Bo Nilson

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引用次数: 0

摘要

目的基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）文库对牛链球菌/马链球菌复合体（SBSEC）亚种的鉴定能力有限。目的是开发和验证适用于诊断培养和采样条件的新型MALDI-TOF质谱文库，以改进SBSEC亚种的鉴定。方法采用全基因组测序方法鉴定ssbsec分离株，作为参考标准。在血琼脂板（sbseccmr - bae）和巧克力琼脂板（sbseccmr - can）厌氧条件下培养的53株分离菌构建了2个文库。用119个独特的分离株对Bruker MALDI Biotyper (MBT) Compass文库和SBSECCMRS文库的性能进行了验证。在两种不同的培养条件下，采用直接转移、靶提取和全提取方法对每个分离物进行取样，总共有714种不同的分离物和条件。结果在所有培养条件和方法下，MBT compass文库共鉴定出463/714个亚种（65%），SBSECCMRS文库鉴定出674/698个亚种（97%）。sbseccmr - bae文库和sbseccmr - can文库分别鉴定出707/713（99%）和684/706（97%）个亚种。无论采用何种培养条件或三种样品制备方法，sbsec - cmr - bae文库都表现最佳，亚种鉴定准确率高达100%。结论新的SBSEC- cmr - bae文库在SBSEC亚种鉴定中表现优异，提高了MALDI-TOF质谱鉴定的性能。它可以直接实施到常规临床实践中，在那里使用布鲁克MALDI生物类型仪。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Creation and validation of improved MALDI-TOF MS libraries for S. bovis-S. equinus-complex subspecies identification adapted to diagnostic culturing and extraction conditions

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Creation and validation of improved MALDI-TOF MS libraries for S. bovis-S. equinus-complex subspecies identification adapted to diagnostic culturing and extraction conditions

Objectives

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) libraries have limited capabilities for identification of Streptococcus bovis/Streptococcus equinus-complex (SBSEC) subspecies. The objective was to develop and validate novel MALDI-TOF MS libraries adapted to diagnostic culturing and sampling conditions for improved identification of SBSEC subspecies.

Methods

SBSEC isolates had previously been identified by whole genome sequencing, constituting the reference standard. Two libraries were constructed based on 53 isolates cultured on blood agar plates under aerobic conditions (SBSEC-CMRS-BAE) and on chocolate agar under anaerobic conditions (SBSECCMRS-CAN). The performances of the new libraries, the Bruker MALDI Biotyper (MBT) Compass Library and the previously developed SBSECCMRS library based on isolates cultured in brain heart infusion-broth, were validated with 119 unique isolates. Each isolate was sampled using the direct transfer, on-target extraction, and full extraction methods under the two different culture conditions – in total 714 different isolates and conditions.

Results

With a cutoff identification score of ≥2.0, the MBT compass library identified subspecies in 463/714 (65 %) in total for all culturing conditions and methods, while the broth-based SBSECCMRS library identified 674/698 (97 %). The novel SBSEC-CMRS-BAE and SBSECCMRS-CAN libraries identified subspecies in 707/713 (99 %) and 684/706 (97 %), respectively. The SBSEC-CMRS-BAE library performed best, regardless of the culturing conditions used or the three sample preparation methods employed, with up to 100 % correct subspecies identifications.

Conclusions

The novel SBSEC-CMRS-BAE library performed excellently and improved MALDI-TOF MS performance in identifying SBSEC subspecies. It can be implemented directly into routine clinical practice where the Bruker MALDI Biotyper is used.

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来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.