Jiujun Du , Xinglu Zhou , Hantian Wei , Yongxia Bai , Xueqin Song , Lei Zhang , Jianjun Hu
{"title":"杨树PdRabG3f通过促进内源脱落酸合成抑制根伸长和提高耐盐性","authors":"Jiujun Du , Xinglu Zhou , Hantian Wei , Yongxia Bai , Xueqin Song , Lei Zhang , Jianjun Hu","doi":"10.1016/j.plaphy.2025.110233","DOIUrl":null,"url":null,"abstract":"<div><div>Proteins belonging to the small GTPases family, particularly Rab proteins, are involved in plant root development. The RabG subfamily proteins, in particular, play a role in regulating plant stress responses. This study revealed that the poplar RabG protein PdRabG3f exhibited tissue-specific activity in root epidermis, root hairs, and vascular stele. Functional characterization further demonstrated that overexpression of <em>PdRabG3f</em> in transgenic poplar significantly inhibited root elongation, reducing maximum root length, and fresh root weight, whereas suppression of <em>PdRabG3f</em> enhanced root growth — a phenotype consistent with its spatial expression pattern. In <em>PdRabG3f</em> overexpression lines, elevated endogenous abscisic acid (ABA) levels were observed, which correlated with enhanced ABA sensitivity and further inhibition of root elongation under exogenous ABA treatment. As a result, these lines exhibited improved resilience under salt stress, including higher peroxidase activity, reduced malondialdehyde content, and maintained relative water content. Transcriptome analysis further supported their enhanced salt tolerance, revealing significant upregulation of stress-responsive genes and enrichment in stress response pathways. These findings establish <em>PdRabG3f</em> as a negative regulator of root growth via ABA biosynthesis and highlight its role in enhancing salt tolerance through ABA-dependent stress signaling. This study provides novel insights into Rab protein-mediated hormonal regulation in woody plants, offering potential targets for improving stress resilience in poplar.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"228 ","pages":"Article 110233"},"PeriodicalIF":5.7000,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Poplar PdRabG3f inhibits root elongation and increases salt tolerance by enhancing endogenous abscisic acid synthesis\",\"authors\":\"Jiujun Du , Xinglu Zhou , Hantian Wei , Yongxia Bai , Xueqin Song , Lei Zhang , Jianjun Hu\",\"doi\":\"10.1016/j.plaphy.2025.110233\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Proteins belonging to the small GTPases family, particularly Rab proteins, are involved in plant root development. The RabG subfamily proteins, in particular, play a role in regulating plant stress responses. This study revealed that the poplar RabG protein PdRabG3f exhibited tissue-specific activity in root epidermis, root hairs, and vascular stele. Functional characterization further demonstrated that overexpression of <em>PdRabG3f</em> in transgenic poplar significantly inhibited root elongation, reducing maximum root length, and fresh root weight, whereas suppression of <em>PdRabG3f</em> enhanced root growth — a phenotype consistent with its spatial expression pattern. In <em>PdRabG3f</em> overexpression lines, elevated endogenous abscisic acid (ABA) levels were observed, which correlated with enhanced ABA sensitivity and further inhibition of root elongation under exogenous ABA treatment. As a result, these lines exhibited improved resilience under salt stress, including higher peroxidase activity, reduced malondialdehyde content, and maintained relative water content. Transcriptome analysis further supported their enhanced salt tolerance, revealing significant upregulation of stress-responsive genes and enrichment in stress response pathways. These findings establish <em>PdRabG3f</em> as a negative regulator of root growth via ABA biosynthesis and highlight its role in enhancing salt tolerance through ABA-dependent stress signaling. This study provides novel insights into Rab protein-mediated hormonal regulation in woody plants, offering potential targets for improving stress resilience in poplar.</div></div>\",\"PeriodicalId\":20234,\"journal\":{\"name\":\"Plant Physiology and Biochemistry\",\"volume\":\"228 \",\"pages\":\"Article 110233\"},\"PeriodicalIF\":5.7000,\"publicationDate\":\"2025-07-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Physiology and Biochemistry\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0981942825007612\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Physiology and Biochemistry","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0981942825007612","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Poplar PdRabG3f inhibits root elongation and increases salt tolerance by enhancing endogenous abscisic acid synthesis
Proteins belonging to the small GTPases family, particularly Rab proteins, are involved in plant root development. The RabG subfamily proteins, in particular, play a role in regulating plant stress responses. This study revealed that the poplar RabG protein PdRabG3f exhibited tissue-specific activity in root epidermis, root hairs, and vascular stele. Functional characterization further demonstrated that overexpression of PdRabG3f in transgenic poplar significantly inhibited root elongation, reducing maximum root length, and fresh root weight, whereas suppression of PdRabG3f enhanced root growth — a phenotype consistent with its spatial expression pattern. In PdRabG3f overexpression lines, elevated endogenous abscisic acid (ABA) levels were observed, which correlated with enhanced ABA sensitivity and further inhibition of root elongation under exogenous ABA treatment. As a result, these lines exhibited improved resilience under salt stress, including higher peroxidase activity, reduced malondialdehyde content, and maintained relative water content. Transcriptome analysis further supported their enhanced salt tolerance, revealing significant upregulation of stress-responsive genes and enrichment in stress response pathways. These findings establish PdRabG3f as a negative regulator of root growth via ABA biosynthesis and highlight its role in enhancing salt tolerance through ABA-dependent stress signaling. This study provides novel insights into Rab protein-mediated hormonal regulation in woody plants, offering potential targets for improving stress resilience in poplar.
期刊介绍:
Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement.
Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB.
Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.