异孢子虫在鹌鹑（Coturnix Coturnix）中的分子流行、系统发育及血液学影响的首次报道

IF 1.4 Q3 PARASITOLOGY

Veterinary parasitology, regional studies and reports Pub Date : 2025-07-12 DOI:10.1016/j.vprsr.2025.101312

Ardas Masood , Muhammad Naeem , Ghafoor Ahmad , Muhammad Farooq , Hira Muqaddas , Mohammed Bourhia , Samir Ibenmoussa , Turki M. Dawoud , Khalid Javed Iqbal , Furhan Iqbal

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引用次数: 0

摘要

本研究调查了2022年至2023年巴基斯坦旁遮普省6个地区（Bahawalnagar、Bahawalpur、Dera Ghazi Khan、Lahore、Layyah和Multan）野生和养殖鹌鹑（Coturnix Coturnix）血样中顶复体寄生虫Isospora sp.的分子流行率和系统发育特征。此外，还记录了寄生虫对宿主全血细胞计数（CBC）的影响。在测试的467只鹌鹑中，有10只（2.1%）显示出Isospora sp的18S rRNA基因扩增。BLAST分析所产生的序列证实了所恢复的PCR产物为Isospora sp。系统发育分析显示，巴基斯坦分离物与全球许多国家的鸟类、爬行动物和哺乳动物报告的Isospora sp聚集在一起。危险因素分析表明，在野生和养殖鸟类之间，寄生虫没有显著差异。各采样点间异孢子虫的总体流行率各不相同，但并不局限于某一特定的鸟类性别。与未感染的鸟类相比，感染鸟类（野生和养殖）的CBC发生了显著变化。本研究首次提供了巴基斯坦鹌鹑中异孢子虫感染的文献资料，强调了在不同地区进行详细调查的必要性，以提高我们对异孢子虫感染率、传播及其对宿主鸟的影响的了解。

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First report of molecular prevalence, phylogeny and hematological impact of Isospora sp. in common quails (Coturnix coturnix)

The present study investigates the molecular prevalence and phylogenetic characteristics of apicomplexan parasite, Isospora sp., in the blood samples of common quails (Coturnix coturnix) sampled from both wild (n = 237) and farmed (n = 230) populations across six districts (Bahawalnagar, Bahawalpur, Dera Ghazi Khan, Lahore, Layyah and Multan) in Punjab, Pakistan, during 2022 to 2023. Additionally, effect of parasite was documented on the complete blood count (CBC) of the hosts. Out of 467 quails tested, 10 (2.1 %) exhibited amplification of the 18S rRNA gene for Isospora sp. BLAST analysis of generated sequences has confirmed the identity of recovered PCR products as Isospora sp. Phylogenetic analysis revealed that Pakistani isolate clustered with Isospora sp. reported from birds, reptiles and mammals in various countries globally. A risk factor analysis indicated that the parasites did not vary significantly when compared between wild and farmed birds. Overall Isospora sp. prevalence varied between the sampling sites but was not limited to a particular bird sex. Significant CBC variations were recorded in infected birds (both wild and farmed) as compared to uninfected ones. This study provides the first documentation of Isospora sp. infection in Pakistani quails, underscoring the need for detailed investigations across different regions to enhance our understanding on Isospora sp. infection rates, their transmission, and impact on the host birds.

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来源期刊

Veterinary parasitology, regional studies and reports Multiple-

CiteScore

2.90

自引率

7.10%

发文量

126

审稿时长

97 days

期刊介绍： Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).