首次成功的牛鼻鳐(爪哇鼻翅目)使用冷冻精液进行子宫内人工授精。

IF 1.1 4区 农林科学 Q3 VETERINARY SCIENCES
Paolo Martelli, Lee Foo Khong, Christopher Marriot Perkins, Jessica Pui Yin Li, Hok Lai Ho, Lell Luk
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引用次数: 0

摘要

繁殖濒危野生动物,包括蓝鳃纲,是现代水族馆的一项基本任务。圈养繁殖计划需要人工授精,以更好地利用每个设施有限的库存。牛鼻鳐(Rhinoptera javanica)成功地进行了人工授精,产生了一个活的后代。在化学约束下收集精液,用定制的溶液延长,并在6℃冷藏24小时。精液的质量在采集时和授精前进行评估,注意颜色、体积、主观精子活力、精子活力和浓度。在化学约束下进行宫内人工授精。监测妊娠是通过连续超声扫描胎儿评估鳃和心跳。经过429天(61周零2天)的妊娠期,一只健康的雌性幼犬出生。遗传微卫星分析证实该幼崽不是孤雌生殖的产物,并确定供体雄性为父亲。这是首例人工授精成功的报道,表明这些技术可以应用于该支系的其他物种。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
First successful intra-uterine artificial insemination in a cownose ray (Rhinoptera javanica) using chilled semen.

Reproduction of endangered wildlife, including elasmobranchs, is a fundamental mission of modern aquariums. Captive breeding programs necessitate artificial insemination to better utilize the limited stock in each facility. A cownose ray (Rhinoptera javanica) was successfully inseminated producing a live offspring. The semen was collected under chemical restraint, extended with a custom-made solution and kept chilled at 6oC for 24 hr. The quality of the semen was assessed at collection and just prior to insemination, noting color, volume, subjective sperm motility, sperm viability and concentration. Intrauterine insemination was carried out under chemical restraint. The monitoring of pregnancy was performed by serial ultrasound scans of the fetus assessing gilling and heartbeat. A healthy female pup was born after a gestation of 429 days (61 weeks and 2 days). Genetic microsatellite analysis confirmed that the pup was not a product of parthenogenesis and identified the donor male as the sire. This is the first report of a successful artificial insemination in an aplacental viviparous ray, suggesting these techniques can be applied to other species in this clade.

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来源期刊
Journal of Veterinary Medical Science
Journal of Veterinary Medical Science 农林科学-兽医学
CiteScore
2.30
自引率
8.30%
发文量
230
审稿时长
9-18 weeks
期刊介绍: JVMS is a peer-reviewed journal and publishes a variety of papers on veterinary science from basic research to applied science and clinical research. JVMS is published monthly and consists of twelve issues per year. Papers are from the areas of anatomy, physiology, pharmacology, toxicology, pathology, immunology, microbiology, virology, parasitology, internal medicine, surgery, clinical pathology, theriogenology, avian disease, public health, ethology, and laboratory animal science. Although JVMS has played a role in publishing the scientific achievements of Japanese researchers and clinicians for many years, it now also accepts papers submitted from all over the world.
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