描述应激类似的低剂量皮质醇暴露对人声带成纤维细胞的影响。

IF 2.5 4区 医学 Q1 AUDIOLOGY & SPEECH-LANGUAGE PATHOLOGY
Anumitha Venkatraman, Wen-Hsuan Tseng, Susan Thibeault
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引用次数: 0

摘要

目的:社会心理压力导致血浆中循环糖皮质激素的增加,血浆流经全身。目前尚不清楚应激诱导的糖皮质激素暴露是否会改变人类声带成纤维细胞(hVFF)的生物学机制。本研究描述了应激模拟皮质醇暴露(100 nM)对体外健康hVFF和脂多糖(LPS)处理的hVFF的影响。方法:我们将hVFF暴露于四种条件下:皮质醇处理(100 nM, 7天),lps处理,皮质醇+ lps联合处理,以及未处理的对照组。获得炎性基因(il - 1-β、TNF-α、转化生长因子[TGF]-β1)、糖皮质激素信号(11β HSD1、糖皮质激素诱导亮氨酸拉链[GILZ])、纤维化基因(ACTA2)的相对表达。免疫细胞化学检测α-平滑肌肌动蛋白表达(α-SMA)。结果:与未处理的对照组相比,应激类皮质醇暴露(皮质醇处理的细胞)显著上调GILZ和促纤维化标志物(ACTA2)的基因表达,下调il - 1-β的表达(P < 0.05)。与未处理的对照组细胞相比,lps处理的细胞炎症因子的表达显著上调(il - 1-β, P = 0.003;Tnf -α, p < 0.001;TGF-β1, p = 0.031)。与lps处理的细胞相比,皮质醇+ lps处理的细胞上调了il - 1-β的表达(P = 0.028)。免疫细胞化学显示α-SMA仅在皮质醇+ lps处理的细胞中呈阳性表达。结论:应激类皮质醇暴露可抑制hVFF的炎症细胞因子,上调糖皮质激素信号,以及促纤维化基因靶点。应激模拟皮质醇暴露和LPS联合上调炎症因子、糖皮质激素信号基因表达和α-SMA蛋白表达。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Delineating Effects of Stress-Analogous, Low-Dose Cortisol Exposure on Human Vocal Fold Fibroblasts.

Objective: Psychosocial stress results in an increase of circulating glucocorticoids in blood plasma that flows throughout the body. It is unclear whether stress-induced glucocorticoid exposure alters biological mechanisms of human vocal fold fibroblasts (hVFF). This study delineated the effects of stress-analogous cortisol exposure (100 nM) on healthy hVFF and lipopolysaccharide (LPS)-treated hVFF, in vitro.

Methods: We exposed hVFF to four conditions - cortisol-treated (100 nM, 7 days), LPS-treated, combined cortisol + LPS-treated, and an untreated control. Relative gene expression of inflammatory (IL1-β, TNF-α, transforming growth factor [TGF]-β1), glucocorticoid signaling (11β HSD1, glucocorticoid-induced leucine zipper [GILZ]), and fibrotic gene expression (ACTA2) was obtained. Alpha smooth muscle actin protein expression (α-SMA) was also measured via immunocytochemistry.

Results: Stress-analogous cortisol exposure (cortisol-treated cells) significantly upregulated gene expression of GILZ and pro-fibrotic marker (ACTA2) and downregulated expression of IL1-β when compared to untreated controls (P < 0.05). LPS-treated cells significantly upregulated expression of inflammatory cytokines compared to untreated control cells (IL1-β, P = 0.003; TNF-α, P < 0.001; TGF-β1, p = 0.031). Cortisol + LPS-treated cells upregulated expression of IL1-β, when compared to LPS-treated cells (P = 0.028). Immunocytochemistry revealed positive expression of α-SMA in the cortisol + LPS-treated cells only.

Conclusion: Stress-analogous cortisol exposure inhibits inflammatory cytokines, upregulates glucocorticoid signaling, and pro-fibrotic gene targets in hVFF. The combination of stress-analogous cortisol exposure and LPS upregulated inflammatory cytokines, glucocorticoid signaling gene expression, and protein expression of α-SMA.

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来源期刊
Journal of Voice
Journal of Voice 医学-耳鼻喉科学
CiteScore
4.00
自引率
13.60%
发文量
395
审稿时长
59 days
期刊介绍: The Journal of Voice is widely regarded as the world''s premiere journal for voice medicine and research. This peer-reviewed publication is listed in Index Medicus and is indexed by the Institute for Scientific Information. The journal contains articles written by experts throughout the world on all topics in voice sciences, voice medicine and surgery, and speech-language pathologists'' management of voice-related problems. The journal includes clinical articles, clinical research, and laboratory research. Members of the Foundation receive the journal as a benefit of membership.
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