大肠杆菌来源的外膜囊泡通过调控凋亡对结直肠癌细胞的抗癌作用。

IF 1.6 4区 医学 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH
Marjan Ghiyasvand, Ardeshir Hesampour, Hossein Dabiri, Javad Arasteh
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引用次数: 0

摘要

背景:结直肠癌是最致命的癌症之一,预后差,治疗选择有限。微生物群及其代谢产物,包括毒素,已被证明具有抗肿瘤特性。大肠杆菌的外膜囊泡(OMVs)可能引发肠细胞炎症。我们旨在探讨大肠杆菌衍生的omv通过促进细胞凋亡和焦亡,特别是通过在CT-26细胞系中过表达GSDMD,对结直肠癌细胞的抗肿瘤作用。方法:2022年从德黑兰Histogenotech公司获得5株非复制型大肠杆菌,对其omv进行分离鉴定。利用CT-26细胞系研究大肠杆菌源性omv的细胞毒性和凋亡作用。采用Real-time PCR和western blotting检测细胞凋亡和焦亡标志物(GSDMD、NLRP3、ROCK)的表达。结果:大肠杆菌菌株产生直径5 ~ 200nm的纳米囊泡;10µg浓度的OMVs显著降低CT-26细胞活力,诱导细胞凋亡。GSDMD和NLRP3表达升高,ROCK表达下调。结论:大肠杆菌衍生的omv可引发炎症过程,对结直肠癌细胞产生负面影响。需要进一步的研究来评估它们治疗结直肠癌的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Anticancer Effects of <i>Escherichia Coli</i>-Derived Outer Membrane Vesicles against Colorectal Cancer Cells through Regulation of Apoptosis.

Anticancer Effects of <i>Escherichia Coli</i>-Derived Outer Membrane Vesicles against Colorectal Cancer Cells through Regulation of Apoptosis.

Anticancer Effects of <i>Escherichia Coli</i>-Derived Outer Membrane Vesicles against Colorectal Cancer Cells through Regulation of Apoptosis.

Anticancer Effects of Escherichia Coli-Derived Outer Membrane Vesicles against Colorectal Cancer Cells through Regulation of Apoptosis.

Background: Colorectal cancer is one of the deadliest cancers with poor prognosis and limited treatment options. Microbiota and their metabolites, including toxins, have been shown to possess anti-tumor properties. Outer membrane vesicles (OMVs) from Escherichia coli may trigger inflammation in intestinal cells. We aimed to explore the anti-cancer effects of E. coli-derived OMVs on colorectal cancer cells by promoting apoptosis and pyroptosis, particularly through the over-expression of GSDMD in the CT-26 cell line.

Methods: In 2022, five non-replicating probiotic strains of E. coli were obtained from the Histogenotech Company in Tehran, and their OMVs were isolated and characterized. The CT-26 cell line was used to assess cell toxicity and the apoptotic effects of E. coli-derived OMVs. Real-time PCR and western blotting were performed to measure the expression of apoptosis and pyroptosis markers (GSDMD, NLRP3, and ROCK).

Results: The E. coli strains produced nanovesicles (5-200 nm in diameter). OMVs significantly reduced the viability of CT-26 cells and induced apoptosis at a concentration of 10 µg. The expression of GSDMD and NLRP3 was increased, while ROCK expression was downregulated in treated colorectal cells.

Conclusion: E. coli-derived OMVs can trigger inflammatory processes and induce negative effects on colorectal cancer cells. Further research is needed to evaluate their potential for colorectal cancer treatment.

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来源期刊
Iranian Journal of Public Health
Iranian Journal of Public Health PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH-
CiteScore
2.20
自引率
7.10%
发文量
300
审稿时长
3-8 weeks
期刊介绍: Iranian Journal of Public Health has been continuously published since 1971, as the only Journal in all health domains, with wide distribution (including WHO in Geneva and Cairo) in two languages (English and Persian). From 2001 issue, the Journal is published only in English language. During the last 41 years more than 2000 scientific research papers, results of health activities, surveys and services, have been published in this Journal. To meet the increasing demand of respected researchers, as of January 2012, the Journal is published monthly. I wish this will assist to promote the level of global knowledge. The main topics that the Journal would welcome are: Bioethics, Disaster and Health, Entomology, Epidemiology, Health and Environment, Health Economics, Health Services, Immunology, Medical Genetics, Mental Health, Microbiology, Nutrition and Food Safety, Occupational Health, Oral Health. We would be very delighted to receive your Original papers, Review Articles, Short communications, Case reports and Scientific Letters to the Editor on the above men­tioned research areas.
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