具有犬瘟热病毒中和活性的小鼠-熊猫嵌合抗体的研制与鉴定。

IF 1.6 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Journal of virological methods Pub Date : 2025-07-11 DOI:10.1016/j.jviromet.2025.115222

Zhengguo Wang , Guishan Ye , Kuijing He , Cong Cai , Anding Zhang , Long Li , Li Han

{"title":"具有犬瘟热病毒中和活性的小鼠-熊猫嵌合抗体的研制与鉴定。","authors":"Zhengguo Wang , Guishan Ye , Kuijing He , Cong Cai , Anding Zhang , Long Li , Li Han","doi":"10.1016/j.jviromet.2025.115222","DOIUrl":null,"url":null,"abstract":"<div><div>Canine distemper (CD) poses as the primary virulent infectious disease threatening the safety of panda populations, and monoclonal antibodies (mAbs) represent a potential therapeutic option for the treatment of CD in pandas. To avoid immune rejection of mouse-derived mAbs in panda clinical treatment, this study utilized a mouse-derived mAb targeting the CDV-H protein to develop a mouse-panda chimeric antibody and established a CHO cell line with stable high-expression of this chimeric antibody. SDS-PAGE results indicated that the chimeric antibody expressed by CHO cells was correctly expressed. Both indirect ELISA and indirect immunofluorescence experiments demonstrated that the chimeric antibody specifically recognized the CDV-H protein and CDV virus. Furthermore, neutralization experiments confirmed its neutralizing effect on CDV. This study successfully prepared the panda-derived chimeric antibody, providing a potential candidate drug reserve for the treatment of CD in pandas.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"338 ","pages":"Article 115222"},"PeriodicalIF":1.6000,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Development and characterization of a mouse-panda chimeric antibody with neutralizing activity against canine distemper virus\",\"authors\":\"Zhengguo Wang , Guishan Ye , Kuijing He , Cong Cai , Anding Zhang , Long Li , Li Han\",\"doi\":\"10.1016/j.jviromet.2025.115222\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Canine distemper (CD) poses as the primary virulent infectious disease threatening the safety of panda populations, and monoclonal antibodies (mAbs) represent a potential therapeutic option for the treatment of CD in pandas. To avoid immune rejection of mouse-derived mAbs in panda clinical treatment, this study utilized a mouse-derived mAb targeting the CDV-H protein to develop a mouse-panda chimeric antibody and established a CHO cell line with stable high-expression of this chimeric antibody. SDS-PAGE results indicated that the chimeric antibody expressed by CHO cells was correctly expressed. Both indirect ELISA and indirect immunofluorescence experiments demonstrated that the chimeric antibody specifically recognized the CDV-H protein and CDV virus. Furthermore, neutralization experiments confirmed its neutralizing effect on CDV. This study successfully prepared the panda-derived chimeric antibody, providing a potential candidate drug reserve for the treatment of CD in pandas.</div></div>\",\"PeriodicalId\":17663,\"journal\":{\"name\":\"Journal of virological methods\",\"volume\":\"338 \",\"pages\":\"Article 115222\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2025-07-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of virological methods\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0166093425001156\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of virological methods","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0166093425001156","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}

引用次数: 0

摘要

犬瘟热（CD）是威胁大熊猫种群安全的主要致命传染病，单克隆抗体（mab）是治疗大熊猫CD的潜在选择。为了避免小鼠源性单克隆抗体在熊猫临床治疗中产生免疫排斥反应，本研究利用针对CDV-H蛋白的小鼠源性单克隆抗体制备小鼠-熊猫嵌合抗体，并建立稳定高表达该嵌合抗体的CHO细胞系。SDS-PAGE结果表明CHO细胞表达的嵌合抗体正确表达。间接ELISA和间接免疫荧光实验表明，嵌合抗体特异性识别CDV- h蛋白和CDV病毒。此外，中和实验证实了其对CDV的中和作用。本研究成功制备了熊猫源性嵌合抗体，为治疗熊猫CD提供了潜在的候选药物储备。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Development and characterization of a mouse-panda chimeric antibody with neutralizing activity against canine distemper virus

Canine distemper (CD) poses as the primary virulent infectious disease threatening the safety of panda populations, and monoclonal antibodies (mAbs) represent a potential therapeutic option for the treatment of CD in pandas. To avoid immune rejection of mouse-derived mAbs in panda clinical treatment, this study utilized a mouse-derived mAb targeting the CDV-H protein to develop a mouse-panda chimeric antibody and established a CHO cell line with stable high-expression of this chimeric antibody. SDS-PAGE results indicated that the chimeric antibody expressed by CHO cells was correctly expressed. Both indirect ELISA and indirect immunofluorescence experiments demonstrated that the chimeric antibody specifically recognized the CDV-H protein and CDV virus. Furthermore, neutralization experiments confirmed its neutralizing effect on CDV. This study successfully prepared the panda-derived chimeric antibody, providing a potential candidate drug reserve for the treatment of CD in pandas.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Journal of virological methods 医学-病毒学

CiteScore

5.80

自引率

0.00%

发文量

209

审稿时长

41 days

期刊介绍： The Journal of Virological Methods focuses on original, high quality research papers that describe novel and comprehensively tested methods which enhance human, animal, plant, bacterial or environmental virology and prions research and discovery. The methods may include, but not limited to, the study of: Viral components and morphology- Virus isolation, propagation and development of viral vectors- Viral pathogenesis, oncogenesis, vaccines and antivirals- Virus replication, host-pathogen interactions and responses- Virus transmission, prevention, control and treatment- Viral metagenomics and virome- Virus ecology, adaption and evolution- Applied virology such as nanotechnology- Viral diagnosis with novelty and comprehensive evaluation. We seek articles, systematic reviews, meta-analyses and laboratory protocols that include comprehensive technical details with statistical confirmations that provide validations against current best practice, international standards or quality assurance programs and which advance knowledge in virology leading to improved medical, veterinary or agricultural practices and management.