Comprehensive Genomic, Mutation, Phylogenetic, and Statistical Analysis of the Monkeypox Virus Across Multiple Countries.

Our study offers a comprehensive Monkeypox virus (MPXV) analysis, utilizing innovative protocols to uncover potential vulnerabilities. We examine MPXV's epidemiology, link to smallpox, genomics, mutation landscape, clinical features, diagnostics, therapeutics, FDA-approved drugs, clinical trials, and preventive strategies. We used various analytical methods to analyse genome data from the NCBI database (2019-2023) to assess sequence alignment, protein identification, and genome diversity. The phylogenomic analysis identified unique lineages and a mutation associated with human transmission, revealing that 24 MPXV proteins are particularly mutation-prone. Domain analysis confirmed conserved Ortho-poxvirus genes and critical regions, providing insights into MPXV's structure, diversity, and host interaction. Additionally, we integrated clinical data, geospatial mapping, statistical analysis, and AI/ML for predictive insights on MPXV progression. We also explored bioinformatics tools and plant-based treatments, conducting in-silico studies to identify potential drug targets. Our research integrates epidemiological and genomic data to inform real-time preventive measures and optimize public health responses to MPXV.