揭示巨曲霉抗真菌代谢物对人类真菌病原体的治疗潜力。

IF 2.1 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Indian Journal of Microbiology Pub Date : 2025-06-01 Epub Date: 2025-01-03 DOI:10.1007/s12088-024-01441-0
Karthiga Sivarajan, Ramya Ravindhiran, Jothi Nayaki Sekar, Kavitha Dhandapani
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引用次数: 0

摘要

真菌感染对动物、人类和野生动物的健康构成全球性风险。在病原真菌菌株中,黄曲霉在侵袭性曲霉病(IA)的发病中起着关键作用。一段时间后,致病真菌会对所有获得许可的全身抗真菌药物产生耐药性。真菌病原菌耐药的发生与药物使用不当和病原菌的细胞机制有关。这种担忧可以通过使用抗真菌代谢物来解决。从巨曲霉中提取的抗真菌代谢物由于其治疗潜力而引起了人们的广泛关注。这种特殊的抗真菌蛋白(AFP)在打破致病性真菌的毒力机制方面具有重大的前景。鉴于这些情况,该研究检查了巨型拟南曲霉的甲胎蛋白如何影响曲霉病的病原体黄曲霉。共培养技术充分利用了抗真菌代谢物的潜力来抵消真菌激动剂的作用。拮抗真菌培养滤液处理致病性黄曲霉的生长减少了约91%,证实了培养滤液代谢物对黄曲霉活力的拮抗作用,表明这些代谢物是细胞外的。用培养滤液处理过的黄曲霉的SEM图像中存在萎缩和损坏的孢子,这明显支持其对病原菌细胞膜的影响。培养滤液的MIC和MFC分别为125 μg/ml和250 μg/ml。此外,抗真菌代谢产物的自由基清除活性强调了其显著的抗氧化潜力。最后,细胞毒性研究结果证实了巨角霉抗真菌代谢物的药理作用。因此,该研究强烈支持AFP对黄曲霉的拮抗潜力,提示该代谢物可能成为黄曲霉侵袭性曲霉病的新治疗方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Unraveling the Therapeutic Potential of Antifungal Metabolites of Aspergillus giganteus on Human Fungal Pathogen.

Fungal infections represent a worldwide risk to the health of animals, humans, and wildlife. Among the pathogenic fungal strains, Aspergillus flavus plays a key role in the onset of invasive aspergillosis (IA). Over a while, pathogenic fungi develop resistance to all licensed systemic antifungal drugs. The resistance development in fungal pathogens is attributed to the improper usage of drugs and the inference of the pathogen's cellular mechanisms. This concern could be resolved by the use of antifungal metabolites. Antifungal metabolites derived from Aspergillus giganteus are of profound interest in recent times due to their therapeutic potential. This particular antifungal protein (AFP) holds substantial promise in breaking the virulence mechanism in pathogenic fungi. In light of these circumstances, the study examined how AFP from A. giganteus affects A. flavus, the pathogen responsible for aspergillosis. The co-culturing technique firmly drew the potential of antifungal metabolites to counteract the effects of fungal agonists. A reduction of approximately 91% in the growth of pathogenic A. flavus treated with culture filtrates of antagonist fungi confirms the antagonistic effect of the culture filtrate metabolites on A. flavus viability and indicates that these metabolites are extracellular. The presence of shrunken and damaged spores in the SEM images of A. flavus treated with culture filtrates is evident in supporting its impact on the cellular membrane of the pathogenic bacteria. The MIC and MFC of culture filtrates were found to be 125 μg/ml and 250 μg/ml. Furthermore, the free radical scavenging activity of antifungal metabolites emphasizes its significant antioxidant potential. Finally, the findings of cytotoxicity studies confirmed the druggability of antifungal metabolites of A. giganteus. Thus, the study strongly endorses the antagonistic potential of AFP against A. flavus, suggesting that this metabolite could be developed into a novel treatment for invasive aspergillosis caused by A. flavus.

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来源期刊
Indian Journal of Microbiology
Indian Journal of Microbiology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MICROBIOLOGY
CiteScore
6.00
自引率
10.00%
发文量
51
审稿时长
1 months
期刊介绍: Indian Journal of Microbiology is the official organ of the Association of Microbiologists of India (AMI). It publishes full-length papers, short communication reviews and mini reviews on all aspects of microbiological research, published quarterly (March, June, September and December). Areas of special interest include agricultural, food, environmental, industrial, medical, pharmaceutical, veterinary and molecular microbiology.
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