基于凝胶电泳的蛋白质形态分离与分析。

Advances in clinical chemistry Pub Date : 2025-01-01 Epub Date: 2025-05-15 DOI:10.1016/bs.acc.2025.04.002
Paul Dowling, Kay Ohlendieck
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引用次数: 0

摘要

蛋白质组学在质谱分析之前使用凝胶电泳(GE)进行高效的蛋白质分离是生物医学化学中常用的以蛋白质形态为中心的分析工具。分离的分子物种可以通过各种蛋白质染色技术可视化,并结合蛋白质相互作用分析和动态翻译后修饰的测定来表征它们。本章描述了一维GE (1D-GE)方法的生物分析用途,包括凝胶电泳液相色谱(GeLC)质谱法,以及常用的二维凝胶电泳(2D-GE)技术,包括用于复杂比较研究的荧光差异GE (DIGE)。讨论了先进的蛋白质鉴定和基于凝胶的蛋白质组学可以帮助发现新的生物标志物候选物的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Gel electrophoresis-based proteoform separation and analysis.

Proteomics using gel electrophoresis (GE) for efficient protein separation prior to mass spectrometry is a frequently employed and proteoform-centric analysis tool of biomedical chemistry. Isolated molecular species can be visualized by various protein staining techniques and their characterization being combined with protein interaction analyses and the determination of dynamic post-translational modifications. This chapter describes the bioanalytical usefulness of one-dimensional GE (1D-GE) approaches, including the gel electrophoresis liquid chromatography (GeLC) mass spectrometry method, as well as commonly used two-dimensional gel electrophoresis (2D-GE) techniques, including fluorescence difference GE (DIGE) for sophisticated comparative studies. The ways in which advanced protein identification and gel-based proteomics can help to discover novel biomarker candidates is discussed.

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