蓝光处理下,OsCSN2通过GA信号通路调控水稻幼苗表型。

IF 4 2区 生物学 Q1 PLANT SCIENCES
Xinhai Yu, Tongtong Jiao, Changfeng Liu, Hexin Zhang, Yanxi Liu, Chunyu Zhang, Ming Wu, Liquan Guo
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引用次数: 0

摘要

蓝光是影响植物光形态发生、调节植物生长发育的重要环境信号。COP9信号复合物(CSN)是一种多亚基蛋白复合物,在调节光形态发生中起着关键作用,其中CSN2被认为是CSN组装和功能所必需的关键亚基。本研究探讨了蓝光条件下OsCSN2在水稻中的作用。利用OsCSN2敲除(KO)突变体植株和野生型(WT)和突变型OsCSN2的转基因过表达(OE)系,我们观察到蓝光下OsCSN2对幼苗整体表型的显著抑制,表明OsCSN2在蓝光介导的形态发生中起负调控作用。进一步分析表明,外源施用赤霉素(GA3)和赤霉素合成抑制剂多效唑(paclobutrazol, PAC)可以调节幼苗对蓝光的响应伸长,特别是影响植株高度、胚芽鞘和第一次不完整叶长,但不改变根系生长。这表明OsCSN2通过赤霉素信号通路介导蓝光对地上发育的抑制作用。第9天,结合Western blotting (WB)和qRT-PCR (qRT-PCR)分析内源性GA3水平发现,OsCSN2通过cryptochrome 2 (CRY2)感知蓝光信号,影响COP1和BBX14的表达,并突出其在光敏信号通路中的作用。这种调控最终影响GA信号通路中SLR1的降解,影响水稻幼苗的生长发育。我们的研究结果还强调了CSN中OsCSN1和OsCSN2在调节水稻幼苗光形态发生中的不同作用,从而为植物对蓝光反应的复杂调控机制提供了新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Under Blue Light Treatment, OsCSN2 Regulates the Phenotype of Rice Seedlings Through the GA Signaling Pathway.

Blue light is a significant environmental cue influencing plant photomorphogenesis and regulating plant growth and development. The COP9 signaling complex (CSN), a multi-subunit protein complex, plays a pivotal role in regulating photomorphogenesis, with CSN2 being identified as a key subunit essential for the assembly and function of the CSN. This study investigated the role of OsCSN2 in rice under blue-light conditions. Utilizing OsCSN2 knockout (KO) mutant plants and transgenic overexpression (OE) lines for wild-type (WT) and mutated versions of OsCSN2, we observed significant suppression of the overall seedling phenotype under blue light, indicating that OsCSN2 acts as a negative regulator of blue light-mediated morphogenesis. Further analysis revealed that exogenous application of gibberellin (GA3) and the GA synthesis inhibitor paclobutrazol (PAC) modulated seedling elongation in response to blue light, particularly affecting plant height, coleoptile, and first incomplete leaf length without altering root growth. This suggests that OsCSN2 mediates the inhibitory effects of blue light on aboveground development through the gibberellin signaling pathway. On day 9, the analyses of endogenous GA3 levels combined with Western blotting (WB) and quantitative real-time PCR (qRT-PCR) revealed that OsCSN2 senses blue light signals through cryptochrome 2 (CRY2), influences the expression of COP1 and BBX14, and highlights its role in the photoreceptive signaling pathway. This regulation ultimately influences the degradation of SLR1 within the GA signaling pathway, affecting rice seedling growth and development. Our findings also highlight the differential roles of OsCSN1 and OsCSN2 within the CSN in modulating rice seedling photomorphogenesis, thereby providing new insights into the intricate regulatory mechanisms governing plant responses to blue light.

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来源期刊
Plants-Basel
Plants-Basel Agricultural and Biological Sciences-Ecology, Evolution, Behavior and Systematics
CiteScore
6.50
自引率
11.10%
发文量
2923
审稿时长
15.4 days
期刊介绍: Plants (ISSN 2223-7747), is an international and multidisciplinary scientific open access journal that covers all key areas of plant science. It publishes review articles, regular research articles, communications, and short notes in the fields of structural, functional and experimental botany. In addition to fundamental disciplines such as morphology, systematics, physiology and ecology of plants, the journal welcomes all types of articles in the field of applied plant science.
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