大豆苷元的心脏保护作用:探索NRG-1/Akt通路在异丙肾上腺素诱导的大鼠心肌梗死模型中的作用。

IF 3.3 3区 医学 Q2 CARDIAC & CARDIOVASCULAR SYSTEMS
Işık Tekin, Gulsah Gundogdu, Ozgen Kilic-Erkek, Ipek Buber, Hasan Akca, Yalın Tolga Yaylali, Gulcin Abban-Mete
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引用次数: 0

摘要

背景与目的:本研究旨在评估抗氧化抗炎化合物大豆苷元对大鼠异丙肾上腺素(ISO)诱导的心肌梗死(MI)模型的心脏保护作用,重点研究其对NRG-1/Akt信号通路的影响。方法与结果:雄性Sprague Dawley大鼠28只,随机分为对照组、MI组、MI + DMSO组和MI +大豆苷元组。用ISO (85 mg/kg)皮下诱导心肌梗死2次,间隔24 h。心肌梗死后7天,每天腹腔注射大豆苷元(10mg /kg)。心肌梗死后24 h测定血清肌钙蛋白以确认损伤。采用ELISA法分析心脏组织总抗氧化状态(TAS)、总氧化状态(TOS)、神经调节蛋白-1 (NRG-1)、红母细胞白血病病毒致癌基因同源物2 (ErbB2)和蛋白激酶B (Akt)水平。进行组织病理学和免疫组织化学评价。心肌梗死组的TOS和氧化稳定指数(oxidative stability index, OSI)水平显著高于对照组(P)。结论:大豆苷元可能通过激活NRG-1/Akt通路,降低心肌梗死后的氧化应激,改善心脏分子和结构参数,从而发挥心脏保护作用。其治疗心血管疾病的潜力有待进一步研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cardioprotective effects of daidzein: Exploring the role of the NRG-1/Akt pathway in a rat model of isoproterenol-induced myocardial infarction.

Background and aims: This study aimed to evaluate the cardioprotective effects of daidzein, an antioxidant and anti-inflammatory compound, focusing on its impact on the NRG-1/Akt signaling pathway in a rat model of isoproterenol (ISO)-induced myocardial infarction (MI).

Methods and results: Twenty-eight male Sprague Dawley rats were divided into four groups: Control, MI, MI + DMSO, and MI + Daidzein. MI was induced with ISO (85 mg/kg) subcutaneously twice with a 24-h intervals. Daidzein was administered intraperitoneally (10 mg/kg) daily for seven days post-MI. Serum troponin were measured 24 h after MI to confirm injury. Cardiac tissues were analyzed for total antioxidant status (TAS), total oxidant status (TOS), Neuregulin-1 (NRG-1), erythroblastic leukemia viral oncogene homolog 2 (ErbB2), and Protein Kinase B (Akt) levels using ELISA. Histopathological and immunohistochemical evaluations were conducted. TOS and oxidative stablity index (OSI) levels were significantly higher in the MI group compared to the Control (P < 0.05), but daidzein treatment significantly reduced these levels (P = 0.014, P = 0.036). NRG-1 and ErbB2 levels were decreased in the MI group compared to Control (P = 0.029, P = 0.001) but restored by daidzein (P = 0.026, P = 0.01). Immunohistochemistry showed increased NRG-1 and ErbB2 in cardiac tissue following daidzein treatment, and histopathology confirmed reduced inflammation, damage, and fibrosis (P < 0.05).

Conclusion: Daidzein demonstrated cardioprotective effects by reducing oxidative stress and improving molecular and structural cardiac parameters post-MI, likely through activation of the NRG-1/Akt pathway. Further research is needed to explore its therapeutic potential in cardiovascular disease.

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来源期刊
CiteScore
6.80
自引率
2.60%
发文量
332
审稿时长
57 days
期刊介绍: Nutrition, Metabolism & Cardiovascular Diseases is a forum designed to focus on the powerful interplay between nutritional and metabolic alterations, and cardiovascular disorders. It aims to be a highly qualified tool to help refine strategies against the nutrition-related epidemics of metabolic and cardiovascular diseases. By presenting original clinical and experimental findings, it introduces readers and authors into a rapidly developing area of clinical and preventive medicine, including also vascular biology. Of particular concern are the origins, the mechanisms and the means to prevent and control diabetes, atherosclerosis, hypertension, and other nutrition-related diseases.
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