{"title":"大豆苷元的心脏保护作用:探索NRG-1/Akt通路在异丙肾上腺素诱导的大鼠心肌梗死模型中的作用。","authors":"Işık Tekin, Gulsah Gundogdu, Ozgen Kilic-Erkek, Ipek Buber, Hasan Akca, Yalın Tolga Yaylali, Gulcin Abban-Mete","doi":"10.1016/j.numecd.2025.104204","DOIUrl":null,"url":null,"abstract":"<p><strong>Background and aims: </strong>This study aimed to evaluate the cardioprotective effects of daidzein, an antioxidant and anti-inflammatory compound, focusing on its impact on the NRG-1/Akt signaling pathway in a rat model of isoproterenol (ISO)-induced myocardial infarction (MI).</p><p><strong>Methods and results: </strong>Twenty-eight male Sprague Dawley rats were divided into four groups: Control, MI, MI + DMSO, and MI + Daidzein. MI was induced with ISO (85 mg/kg) subcutaneously twice with a 24-h intervals. Daidzein was administered intraperitoneally (10 mg/kg) daily for seven days post-MI. Serum troponin were measured 24 h after MI to confirm injury. Cardiac tissues were analyzed for total antioxidant status (TAS), total oxidant status (TOS), Neuregulin-1 (NRG-1), erythroblastic leukemia viral oncogene homolog 2 (ErbB2), and Protein Kinase B (Akt) levels using ELISA. Histopathological and immunohistochemical evaluations were conducted. TOS and oxidative stablity index (OSI) levels were significantly higher in the MI group compared to the Control (P < 0.05), but daidzein treatment significantly reduced these levels (P = 0.014, P = 0.036). NRG-1 and ErbB2 levels were decreased in the MI group compared to Control (P = 0.029, P = 0.001) but restored by daidzein (P = 0.026, P = 0.01). Immunohistochemistry showed increased NRG-1 and ErbB2 in cardiac tissue following daidzein treatment, and histopathology confirmed reduced inflammation, damage, and fibrosis (P < 0.05).</p><p><strong>Conclusion: </strong>Daidzein demonstrated cardioprotective effects by reducing oxidative stress and improving molecular and structural cardiac parameters post-MI, likely through activation of the NRG-1/Akt pathway. Further research is needed to explore its therapeutic potential in cardiovascular disease.</p>","PeriodicalId":49722,"journal":{"name":"Nutrition Metabolism and Cardiovascular Diseases","volume":" ","pages":"104204"},"PeriodicalIF":3.3000,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Cardioprotective effects of daidzein: Exploring the role of the NRG-1/Akt pathway in a rat model of isoproterenol-induced myocardial infarction.\",\"authors\":\"Işık Tekin, Gulsah Gundogdu, Ozgen Kilic-Erkek, Ipek Buber, Hasan Akca, Yalın Tolga Yaylali, Gulcin Abban-Mete\",\"doi\":\"10.1016/j.numecd.2025.104204\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background and aims: </strong>This study aimed to evaluate the cardioprotective effects of daidzein, an antioxidant and anti-inflammatory compound, focusing on its impact on the NRG-1/Akt signaling pathway in a rat model of isoproterenol (ISO)-induced myocardial infarction (MI).</p><p><strong>Methods and results: </strong>Twenty-eight male Sprague Dawley rats were divided into four groups: Control, MI, MI + DMSO, and MI + Daidzein. MI was induced with ISO (85 mg/kg) subcutaneously twice with a 24-h intervals. Daidzein was administered intraperitoneally (10 mg/kg) daily for seven days post-MI. Serum troponin were measured 24 h after MI to confirm injury. Cardiac tissues were analyzed for total antioxidant status (TAS), total oxidant status (TOS), Neuregulin-1 (NRG-1), erythroblastic leukemia viral oncogene homolog 2 (ErbB2), and Protein Kinase B (Akt) levels using ELISA. Histopathological and immunohistochemical evaluations were conducted. TOS and oxidative stablity index (OSI) levels were significantly higher in the MI group compared to the Control (P < 0.05), but daidzein treatment significantly reduced these levels (P = 0.014, P = 0.036). NRG-1 and ErbB2 levels were decreased in the MI group compared to Control (P = 0.029, P = 0.001) but restored by daidzein (P = 0.026, P = 0.01). Immunohistochemistry showed increased NRG-1 and ErbB2 in cardiac tissue following daidzein treatment, and histopathology confirmed reduced inflammation, damage, and fibrosis (P < 0.05).</p><p><strong>Conclusion: </strong>Daidzein demonstrated cardioprotective effects by reducing oxidative stress and improving molecular and structural cardiac parameters post-MI, likely through activation of the NRG-1/Akt pathway. Further research is needed to explore its therapeutic potential in cardiovascular disease.</p>\",\"PeriodicalId\":49722,\"journal\":{\"name\":\"Nutrition Metabolism and Cardiovascular Diseases\",\"volume\":\" \",\"pages\":\"104204\"},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2025-06-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nutrition Metabolism and Cardiovascular Diseases\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1016/j.numecd.2025.104204\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CARDIAC & CARDIOVASCULAR SYSTEMS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nutrition Metabolism and Cardiovascular Diseases","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.numecd.2025.104204","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CARDIAC & CARDIOVASCULAR SYSTEMS","Score":null,"Total":0}
Cardioprotective effects of daidzein: Exploring the role of the NRG-1/Akt pathway in a rat model of isoproterenol-induced myocardial infarction.
Background and aims: This study aimed to evaluate the cardioprotective effects of daidzein, an antioxidant and anti-inflammatory compound, focusing on its impact on the NRG-1/Akt signaling pathway in a rat model of isoproterenol (ISO)-induced myocardial infarction (MI).
Methods and results: Twenty-eight male Sprague Dawley rats were divided into four groups: Control, MI, MI + DMSO, and MI + Daidzein. MI was induced with ISO (85 mg/kg) subcutaneously twice with a 24-h intervals. Daidzein was administered intraperitoneally (10 mg/kg) daily for seven days post-MI. Serum troponin were measured 24 h after MI to confirm injury. Cardiac tissues were analyzed for total antioxidant status (TAS), total oxidant status (TOS), Neuregulin-1 (NRG-1), erythroblastic leukemia viral oncogene homolog 2 (ErbB2), and Protein Kinase B (Akt) levels using ELISA. Histopathological and immunohistochemical evaluations were conducted. TOS and oxidative stablity index (OSI) levels were significantly higher in the MI group compared to the Control (P < 0.05), but daidzein treatment significantly reduced these levels (P = 0.014, P = 0.036). NRG-1 and ErbB2 levels were decreased in the MI group compared to Control (P = 0.029, P = 0.001) but restored by daidzein (P = 0.026, P = 0.01). Immunohistochemistry showed increased NRG-1 and ErbB2 in cardiac tissue following daidzein treatment, and histopathology confirmed reduced inflammation, damage, and fibrosis (P < 0.05).
Conclusion: Daidzein demonstrated cardioprotective effects by reducing oxidative stress and improving molecular and structural cardiac parameters post-MI, likely through activation of the NRG-1/Akt pathway. Further research is needed to explore its therapeutic potential in cardiovascular disease.
期刊介绍:
Nutrition, Metabolism & Cardiovascular Diseases is a forum designed to focus on the powerful interplay between nutritional and metabolic alterations, and cardiovascular disorders. It aims to be a highly qualified tool to help refine strategies against the nutrition-related epidemics of metabolic and cardiovascular diseases. By presenting original clinical and experimental findings, it introduces readers and authors into a rapidly developing area of clinical and preventive medicine, including also vascular biology. Of particular concern are the origins, the mechanisms and the means to prevent and control diabetes, atherosclerosis, hypertension, and other nutrition-related diseases.