IL-10受体亚基的分子表征。

IF 2.4 3区农林科学 Q1 FISHERIES

Developmental and comparative immunology Pub Date : 2025-07-10 DOI:10.1016/j.dci.2025.105419

Mingming Wenren , Yue Liu , Ying Zhang , Xu Zhou , Dongdong Xu , Changfeng Chi , Zhenming Lü , Huihui Liu

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引用次数: 0

摘要

白细胞介素-10 （IL-10）的跨膜受体复合体，包括IL-10Rα亚基和IL-10Rβ亚基，属于Ⅱ类细胞因子受体家族。在本研究中，我们对白花Nibea albiflora(简称nai - 10r α (MZ620685.1)和nai - 10r β (OK149228.1)的IL-10Rα和IL-10Rβ进行了鉴定，它们的cDNA全长序列分别为2745bp （nai - 10r α）和1570bp (nai - 10r β)，分别含有1755 bp的ORF，编码584 aa蛋白和975 bp的ORF，编码324 aa蛋白。这两个受体亚基都含有两个细胞外纤维连接蛋白型Ⅲ（FNIII）结构域，一个胞内结构域和一个跨膜结构域，这是Ⅱ类细胞因子受体家族的典型特征。Real-time PCR分析显示，nai - 10r α和nai - 10r β在各种健康组织中广泛表达，在免疫相关器官（头肾、脾、肾、肝和鳃）中表达量较高。在感染Poly I:C、副溶血性弧菌和溶藻弧菌后，它们的表达显著上调。作为一种典型的膜受体，nai - 10r α位于HEK293T细胞的细胞膜上，并与nai -10和nai - 10r β位于HEK293T细胞的细胞膜上，表明两者之间可能存在相互作用。成功隔离后。

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Molecular characterization of the receptor subunits of IL-10 in a perciform fish, Nibea albiflora

The transmembrane receptor complex of Interleukin-10 (IL-10), comprising the IL-10Rα subunit and IL-10Rβ subunits, belong to the class Ⅱ cytokine receptor family. In the present study, IL-10Rα and IL-10Rβ were characterized in Nibea albiflora (referred to as NaIL-10Rα (MZ620685.1) and NaIL-10Rβ (OK149228.1)), whose full-length cDNA sequences were 2745bp (NaIL-10Rα) containing an ORF of 1755 bp that encoded a protein of 584 aa, and 1570bp (NaIL-10Rβ) with an ORF 975 bp encoding a protein of 324 aa, respectively. Both receptor subunits contained two extracellular fibronectin type Ⅲ (FNIII) domains, an intracellular domain and a transmembrane part, which are typical features of class Ⅱ cytokine receptor family. Real-time PCR analysis showed that NaIL-10Rα and NaIL-10Rβ were widely expressed in various healthy tissues, with a relatively high expression in immune-related organs (head kidney, spleen, kidney, liver and gill). Their expressions were significantly up-regulated after infection against Poly I:C, Vibrio parahaemolyticus and Vibrio alginolyticus. As a typical membrane receptor, NaIL-10Rα was located on the cell membrane and co-located with NaIL-10 and NaIL-10Rβ on the membrane of HEK293T cells, suggesting the potential interaction between those components. After successful isolation.

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来源期刊

Developmental and comparative immunology 医学-动物学

CiteScore

6.20

自引率

6.90%

发文量

206

审稿时长

49 days

期刊介绍： Developmental and Comparative Immunology (DCI) is an international journal that publishes articles describing original research in all areas of immunology, including comparative aspects of immunity and the evolution and development of the immune system. Manuscripts describing studies of immune systems in both vertebrates and invertebrates are welcome. All levels of immunological investigations are appropriate: organismal, cellular, biochemical and molecular genetics, extending to such fields as aging of the immune system, interaction between the immune and neuroendocrine system and intestinal immunity.