Effects of radish (Raphanus sativus) green extract on the intestinal mucus secretion and prebiotic activity

Dietary imbalances and consumption of highly processed foods can compromise the gut microbiota and weaken intestinal barrier integrity, contributing to leaky gut syndrome. This study aimed to assess the efficacy of radish green extract (RGE) in supporting intestinal health and identify related biomarkers. Using a simulated gastrointestinal model, we evaluated RGE's microstructure changes and its in vitro prebiotic activity. The physiological effects were further examined in mice with obesity induced by a Western diet. RGE supported the proliferation of three probiotic strains, both before and after digestion. In LS174T cells, RGE stimulated MUC2 gene expression. In animal experiments, RGE improved gut barrier function, reduced systemic inflammation, and enhanced microbial diversity. These results suggest that RGE preserves its beneficial properties through digestion and holds promise as a functional food for managing metabolic dysfunction via gut health modulation.