探索结合抑制试验的性能限制

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2025-07-13 DOI:10.1016/j.snb.2025.138321

Erika Hemmerová, Magdalena Čapková, Jiří Homola

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引用次数: 0

摘要

对大多数生物传感器技术来说，直接检测小分子是一个重大挑战。因此，间接检测格式，如结合抑制试验，通常采用。在本研究中，我们利用表面等离子体共振（SPR）生物传感器技术，从理论上和实验上研究了测定参数与半最大抑制浓度（IC50）之间的关系。我们的研究结果表明：(i) IC50随着样品中受体浓度的降低而降低，逐渐接近受体-被分析物相互作用的平衡解离常数，（ii）在检测极限上观察到类似的趋势，这表明所提出的理论可以用来估计最大化生物传感器性能的最佳受体浓度。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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EXPLORING PERFORMANCE LIMITS OF BINDING INHIBITION ASSAYS

Direct detection of small molecules presents a significant challenge for most biosensor technologies. Consequently, indirect detection formats, such as binding inhibition assays, are commonly employed. In this study, we investigate the relationship between assay parameters and the half-maximal inhibitory concentration (IC₅₀), both theoretically and experimentally, using surface plasmon resonance (SPR) biosensor technology. Our results show that (i) IC₅₀ decreases with decreasing receptor concentration in the sample, asymptotically approaching the equilibrium dissociation constant of the receptor–analyte interaction, and (ii) a similar trend is observed for the limit of detection, suggesting that the proposed theory can be used to estimate the optimal receptor concentration for maximizing biosensor performance.

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.