小鼠的多器官转录组学鉴定免疫球蛋白重常数（Ighm）是组织水平的衰老生物标志物

IF 9.4 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2025-07-11 DOI:10.1073/pnas.2423142122

Fan-Qian Yin, Xia-Yan Wang, Yong-Xuan Li, Kai-Jing Li, Si-Yu Ma, Meng-Jiao Lv, Zhen-Hua Liu, Wei-Xia Zhong, Yan Liu, Chuan-Fang Tang, Hong-Shi Liu, Yuze Li, Fu-Hui Xiao, Qing-Peng Kong

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引用次数: 0

摘要

识别适用于多种组织的衰老相关生物标志物具有挑战性，但对于评估组织衰老至关重要。在这里，我们从30只不同年龄的C57BL/6 J小鼠中获得并分析了17个器官上的456个转录组，揭示了大多数老年器官中Ighm、C4b和Ccl8 mrna的持续上调。这一发现得到了独立转录组学和蛋白质组学数据集的支持，并通过western blot、酶联免疫吸附试验（ELISA）和免疫荧光进一步验证，证明Ighm mRNA和蛋白质都是组织水平的衰老生物标志物，至少在小鼠中是这样。其对抗衰老干预的敏感性进一步强调了Ighm在评估小鼠组织衰老中的意义。

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Multiorgan transcriptomics in mice identifies immunoglobulin heavy constant mu ( Ighm ) as a tissue-level aging biomarker

Identifying aging-associated biomarkers applicable for multiple tissues is challenging but crucial for assessing tissue aging. Here, we obtained and analyzed 456 transcriptomes on 17 organs from 30 C57BL/6 J mice with different ages, revealing the consistently upregulated mRNAs of Ighm , C4b , and Ccl8 in most aged organs. This finding received support from independent transcriptomic and proteomic datasets and was further validated through western blot, enzyme-linked immunosorbent assay (ELISA), and immunofluorescence, arguing for both Ighm mRNA and protein as tissue-level aging biomarkers, at least in mice. Its sensitivity to antiaging interventions further emphasizes the significance of Ighm in assessing tissue aging in mice.

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来源期刊

Proceedings of the National Academy of Sciences of the United States of America 综合性期刊-综合性期刊

CiteScore

19.00

自引率

0.90%

发文量

3575

审稿时长

2.5 months

期刊介绍： The Proceedings of the National Academy of Sciences (PNAS), a peer-reviewed journal of the National Academy of Sciences (NAS), serves as an authoritative source for high-impact, original research across the biological, physical, and social sciences. With a global scope, the journal welcomes submissions from researchers worldwide, making it an inclusive platform for advancing scientific knowledge.