从实验数据估计酶活性的一种基于方程的方法。

IF 1.2 4区 教育学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Daniel Ojeda-Del Sol, Anthony Aguiar-Medina, Isel Pascual-Alonso
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引用次数: 0

摘要

酶促反应的研究和分析是表征各种生物系统的必要条件。作为这些程序的一部分,重要的是要了解如何量化这些蛋白质在酶活性方面的生物活性。这项工作提出了一个基于实验数据确定酶活性的一般方程,以及支持它的理论元素。包括一个模型练习,并计算两种酶活性和特定酶活性的方法是详细概述,一步一步。本程序是专门为对酶动力学感兴趣的生物科学本科生设计的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
An Equation-Based Approach to Estimating Enzymatic Activity From Experimental Data.

The study and analysis of enzymatic reactions are essential for characterizing various biological systems. As part of these procedures, it is important to understand how to quantify the biological activity of these proteins in terms of enzymatic activity. This work proposes a general equation for determining enzymatic activity based on experimental data, along with the theoretical elements that support it. A model exercise is included, and the methodology for calculating both enzymatic activity and specific enzymatic activity is outlined in detail, step by step. This procedure is specifically designed for undergraduate students in the biological sciences who are interested in enzyme kinetics.

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来源期刊
Biochemistry and Molecular Biology Education
Biochemistry and Molecular Biology Education 生物-生化与分子生物学
CiteScore
2.60
自引率
14.30%
发文量
99
审稿时长
6-12 weeks
期刊介绍: The aim of BAMBED is to enhance teacher preparation and student learning in Biochemistry, Molecular Biology, and related sciences such as Biophysics and Cell Biology, by promoting the world-wide dissemination of educational materials. BAMBED seeks and communicates articles on many topics, including: Innovative techniques in teaching and learning. New pedagogical approaches. Research in biochemistry and molecular biology education. Reviews on emerging areas of Biochemistry and Molecular Biology to provide background for the preparation of lectures, seminars, student presentations, dissertations, etc. Historical Reviews describing "Paths to Discovery". Novel and proven laboratory experiments that have both skill-building and discovery-based characteristics. Reviews of relevant textbooks, software, and websites. Descriptions of software for educational use. Descriptions of multimedia materials such as tutorials on various aspects of biochemistry and molecular biology.
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