styMdtM基因缺失与修饰λ Red重组方法：对伤寒沙门氏菌多药耐药的影响

IF 1.9 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of microbiological methods Pub Date : 2025-07-08 DOI:10.1016/j.mimet.2025.107191

Anam Tariq , Aqsa Shaheen , Mazhar Iqbal , Moazur Rahman

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引用次数: 0

摘要

styMdtM是伤寒沙门氏菌的多药物外排转运体。在本研究中，采用改良的λ Red重组方法，利用较短的侧臂（约50 bp）在伤寒沙门氏菌基因组中产生styMdtM基因缺失。这种方法有效地敲除了该基因，随后的伤寒沙门氏菌菌株对抗生素相当敏感。

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styMdtM gene deletion with modified λ Red recombineering approach: Implications for multidrug resistance in Salmonella Typhi

styMdtM is a multidrug efflux transporter of Salmonella Typhi. In the present study, a modified λ Red recombineering approach using shorter flanking arms (ca. 50 bp) was used to generate an styMdtM gene deletion in the S. Typhi genome. This approach worked efficiently to knockout the gene and the ensuing S. Typhi strain was considerably more sensitive to antibiotics.

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来源期刊

Journal of microbiological methods 生物-生化研究方法

CiteScore

4.30

自引率

4.50%

发文量

151

审稿时长

29 days

期刊介绍： The Journal of Microbiological Methods publishes scholarly and original articles, notes and review articles. These articles must include novel and/or state-of-the-art methods, or significant improvements to existing methods. Novel and innovative applications of current methods that are validated and useful will also be published. JMM strives for scholarship, innovation and excellence. This demands scientific rigour, the best available methods and technologies, correctly replicated experiments/tests, the inclusion of proper controls, calibrations, and the correct statistical analysis. The presentation of the data must support the interpretation of the method/approach. All aspects of microbiology are covered, except virology. These include agricultural microbiology, applied and environmental microbiology, bioassays, bioinformatics, biotechnology, biochemical microbiology, clinical microbiology, diagnostics, food monitoring and quality control microbiology, microbial genetics and genomics, geomicrobiology, microbiome methods regardless of habitat, high through-put sequencing methods and analysis, microbial pathogenesis and host responses, metabolomics, metagenomics, metaproteomics, microbial ecology and diversity, microbial physiology, microbial ultra-structure, microscopic and imaging methods, molecular microbiology, mycology, novel mathematical microbiology and modelling, parasitology, plant-microbe interactions, protein markers/profiles, proteomics, pyrosequencing, public health microbiology, radioisotopes applied to microbiology, robotics applied to microbiological methods,rumen microbiology, microbiological methods for space missions and extreme environments, sampling methods and samplers, soil and sediment microbiology, transcriptomics, veterinary microbiology, sero-diagnostics and typing/identification.