Claudia Marchán-Moreno , Christian L. Ward-Deitrich , Jasmina Allen , Maite Bueno , David Amouroux , Zoyne Pedrero Zayas , Heidi Goenaga-Infante , Warren T. Corns
{"title":"以HPLC-HG-AFS为重点的硒氨酸定量鉴定多技术平台","authors":"Claudia Marchán-Moreno , Christian L. Ward-Deitrich , Jasmina Allen , Maite Bueno , David Amouroux , Zoyne Pedrero Zayas , Heidi Goenaga-Infante , Warren T. Corns","doi":"10.1016/j.sab.2025.107261","DOIUrl":null,"url":null,"abstract":"<div><div>Selenoneine, a naturally occurring form of selenium (Se) in biota, has recently raised considerable interest in the fields of nutrition, drug development and environmental research. Selenoneine has a strong antioxidant capacity which makes it a potential Se supplement for humans or farm animals. There is a lack of a reliable and cost-effective online speciation methodology for the determination of such a biologically relevant Se species. In this study, a method has been developed for the simultaneous analysis of selenoneine and other organic Se species. It is based on the online coupling of reversed-phase liquid chromatography, ultraviolet radiation, thermal treatment, hydride generation and atomic fluorescence spectrometry (HPLC-UV-TT-HG-AFS). Quantitative determination of selenoneine by external calibration and its separation from commonly found organic Se species (selenomethionine, <em>Se</em>-methyl-selenocysteine) was achieved. The proposed methodology resulted in a linear response (R<sup>2</sup> > 0.999) for a concentration range of 5–100 μgSe L<sup>−1</sup> and a limit of detection of 0.5 μgSe L<sup>−1</sup>. The repeatability was 0.8 %, with a reproducibility of 1.6 %. This analytical approach is versatile, cost-effective, and fast with a chromatographic runtime of less than 12 min. The high selectivity of HPLC-ICP-MS, combined with the species identification power of HPLC-ESI-MS/MS was found invaluable for gaining further speciation insights and confirming the results obtained by HPLC-UV-TT-HG-AFS. The developed approach could serve as useful tool for routine selenoneine analysis in biological samples as shown here with the analysis of seabird liver extracts.</div></div>","PeriodicalId":21890,"journal":{"name":"Spectrochimica Acta Part B: Atomic Spectroscopy","volume":"232 ","pages":"Article 107261"},"PeriodicalIF":3.2000,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A multi-technique platform for the quantification and identification of selenoneine with a focus on HPLC-HG-AFS\",\"authors\":\"Claudia Marchán-Moreno , Christian L. Ward-Deitrich , Jasmina Allen , Maite Bueno , David Amouroux , Zoyne Pedrero Zayas , Heidi Goenaga-Infante , Warren T. Corns\",\"doi\":\"10.1016/j.sab.2025.107261\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Selenoneine, a naturally occurring form of selenium (Se) in biota, has recently raised considerable interest in the fields of nutrition, drug development and environmental research. Selenoneine has a strong antioxidant capacity which makes it a potential Se supplement for humans or farm animals. There is a lack of a reliable and cost-effective online speciation methodology for the determination of such a biologically relevant Se species. In this study, a method has been developed for the simultaneous analysis of selenoneine and other organic Se species. It is based on the online coupling of reversed-phase liquid chromatography, ultraviolet radiation, thermal treatment, hydride generation and atomic fluorescence spectrometry (HPLC-UV-TT-HG-AFS). Quantitative determination of selenoneine by external calibration and its separation from commonly found organic Se species (selenomethionine, <em>Se</em>-methyl-selenocysteine) was achieved. The proposed methodology resulted in a linear response (R<sup>2</sup> > 0.999) for a concentration range of 5–100 μgSe L<sup>−1</sup> and a limit of detection of 0.5 μgSe L<sup>−1</sup>. The repeatability was 0.8 %, with a reproducibility of 1.6 %. This analytical approach is versatile, cost-effective, and fast with a chromatographic runtime of less than 12 min. The high selectivity of HPLC-ICP-MS, combined with the species identification power of HPLC-ESI-MS/MS was found invaluable for gaining further speciation insights and confirming the results obtained by HPLC-UV-TT-HG-AFS. The developed approach could serve as useful tool for routine selenoneine analysis in biological samples as shown here with the analysis of seabird liver extracts.</div></div>\",\"PeriodicalId\":21890,\"journal\":{\"name\":\"Spectrochimica Acta Part B: Atomic Spectroscopy\",\"volume\":\"232 \",\"pages\":\"Article 107261\"},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2025-07-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Spectrochimica Acta Part B: Atomic Spectroscopy\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0584854725001466\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"SPECTROSCOPY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Spectrochimica Acta Part B: Atomic Spectroscopy","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0584854725001466","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"SPECTROSCOPY","Score":null,"Total":0}
A multi-technique platform for the quantification and identification of selenoneine with a focus on HPLC-HG-AFS
Selenoneine, a naturally occurring form of selenium (Se) in biota, has recently raised considerable interest in the fields of nutrition, drug development and environmental research. Selenoneine has a strong antioxidant capacity which makes it a potential Se supplement for humans or farm animals. There is a lack of a reliable and cost-effective online speciation methodology for the determination of such a biologically relevant Se species. In this study, a method has been developed for the simultaneous analysis of selenoneine and other organic Se species. It is based on the online coupling of reversed-phase liquid chromatography, ultraviolet radiation, thermal treatment, hydride generation and atomic fluorescence spectrometry (HPLC-UV-TT-HG-AFS). Quantitative determination of selenoneine by external calibration and its separation from commonly found organic Se species (selenomethionine, Se-methyl-selenocysteine) was achieved. The proposed methodology resulted in a linear response (R2 > 0.999) for a concentration range of 5–100 μgSe L−1 and a limit of detection of 0.5 μgSe L−1. The repeatability was 0.8 %, with a reproducibility of 1.6 %. This analytical approach is versatile, cost-effective, and fast with a chromatographic runtime of less than 12 min. The high selectivity of HPLC-ICP-MS, combined with the species identification power of HPLC-ESI-MS/MS was found invaluable for gaining further speciation insights and confirming the results obtained by HPLC-UV-TT-HG-AFS. The developed approach could serve as useful tool for routine selenoneine analysis in biological samples as shown here with the analysis of seabird liver extracts.
期刊介绍:
Spectrochimica Acta Part B: Atomic Spectroscopy, is intended for the rapid publication of both original work and reviews in the following fields:
Atomic Emission (AES), Atomic Absorption (AAS) and Atomic Fluorescence (AFS) spectroscopy;
Mass Spectrometry (MS) for inorganic analysis covering Spark Source (SS-MS), Inductively Coupled Plasma (ICP-MS), Glow Discharge (GD-MS), and Secondary Ion Mass Spectrometry (SIMS).
Laser induced atomic spectroscopy for inorganic analysis, including non-linear optical laser spectroscopy, covering Laser Enhanced Ionization (LEI), Laser Induced Fluorescence (LIF), Resonance Ionization Spectroscopy (RIS) and Resonance Ionization Mass Spectrometry (RIMS); Laser Induced Breakdown Spectroscopy (LIBS); Cavity Ringdown Spectroscopy (CRDS), Laser Ablation Inductively Coupled Plasma Atomic Emission Spectroscopy (LA-ICP-AES) and Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS).
X-ray spectrometry, X-ray Optics and Microanalysis, including X-ray fluorescence spectrometry (XRF) and related techniques, in particular Total-reflection X-ray Fluorescence Spectrometry (TXRF), and Synchrotron Radiation-excited Total reflection XRF (SR-TXRF).
Manuscripts dealing with (i) fundamentals, (ii) methodology development, (iii)instrumentation, and (iv) applications, can be submitted for publication.