{"title":"光纤传感技术超灵敏检测眼内液中微小核糖核酸用于中枢神经系统淋巴瘤诊断。","authors":"Yanqi Ge,Wenchen Zheng,Zhaoliang Hou,Yule Zhang,Bowen Du,Songrui Wei,Xueyan Liu,Zhi Chen,Han Zhang","doi":"10.1088/1361-6633/adee44","DOIUrl":null,"url":null,"abstract":"MicroRNA (miRNA) in aqueous humor holds significant promise as a non-invasive biomarker for primary central nervous system lymphoma (PCNSL), enabling early diagnosis and prognosis. However, current methods for miRNA detection often suffer from limitations in sensitivity, specificity, and clinical applicability. This study introduces a novel black phosphorus-enhanced fiber-optic surface plasmon resonance sensor (BP-FOSPR) integrated with a CRISPR-Cas13a system for ultrasensitive and single-base-specific detection of
PCNSL-associated miRNA in aqueous humor. The BP nano-interface significantly enhances the surface plasmon resonance signal, while the CRISPR-Cas13a technology enables highly specific detection of miRNA, down to single nucleotide mismatches. This system achieves a detection limit as low as 21aM without the need for amplification and demonstrates robust performance in clinical samples. With its unparalleled sensitivity, specificity, label-free operation, and potential for portability, this biosensing platform offers transformative capabilities for early PCNSL diagnosis, prognosis, and treatment monitoring using minimally invasive liquid biopsy.
.","PeriodicalId":21110,"journal":{"name":"Reports on Progress in Physics","volume":"266 1","pages":""},"PeriodicalIF":19.0000,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Ultra-sensitive detection of microRNA in intraocular fluid using optical fiber sensing technology for central nervous system lymphoma diagnosis.\",\"authors\":\"Yanqi Ge,Wenchen Zheng,Zhaoliang Hou,Yule Zhang,Bowen Du,Songrui Wei,Xueyan Liu,Zhi Chen,Han Zhang\",\"doi\":\"10.1088/1361-6633/adee44\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"MicroRNA (miRNA) in aqueous humor holds significant promise as a non-invasive biomarker for primary central nervous system lymphoma (PCNSL), enabling early diagnosis and prognosis. However, current methods for miRNA detection often suffer from limitations in sensitivity, specificity, and clinical applicability. This study introduces a novel black phosphorus-enhanced fiber-optic surface plasmon resonance sensor (BP-FOSPR) integrated with a CRISPR-Cas13a system for ultrasensitive and single-base-specific detection of
PCNSL-associated miRNA in aqueous humor. The BP nano-interface significantly enhances the surface plasmon resonance signal, while the CRISPR-Cas13a technology enables highly specific detection of miRNA, down to single nucleotide mismatches. This system achieves a detection limit as low as 21aM without the need for amplification and demonstrates robust performance in clinical samples. With its unparalleled sensitivity, specificity, label-free operation, and potential for portability, this biosensing platform offers transformative capabilities for early PCNSL diagnosis, prognosis, and treatment monitoring using minimally invasive liquid biopsy.
.\",\"PeriodicalId\":21110,\"journal\":{\"name\":\"Reports on Progress in Physics\",\"volume\":\"266 1\",\"pages\":\"\"},\"PeriodicalIF\":19.0000,\"publicationDate\":\"2025-07-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Reports on Progress in Physics\",\"FirstCategoryId\":\"101\",\"ListUrlMain\":\"https://doi.org/10.1088/1361-6633/adee44\",\"RegionNum\":1,\"RegionCategory\":\"物理与天体物理\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PHYSICS, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reports on Progress in Physics","FirstCategoryId":"101","ListUrlMain":"https://doi.org/10.1088/1361-6633/adee44","RegionNum":1,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PHYSICS, MULTIDISCIPLINARY","Score":null,"Total":0}
Ultra-sensitive detection of microRNA in intraocular fluid using optical fiber sensing technology for central nervous system lymphoma diagnosis.
MicroRNA (miRNA) in aqueous humor holds significant promise as a non-invasive biomarker for primary central nervous system lymphoma (PCNSL), enabling early diagnosis and prognosis. However, current methods for miRNA detection often suffer from limitations in sensitivity, specificity, and clinical applicability. This study introduces a novel black phosphorus-enhanced fiber-optic surface plasmon resonance sensor (BP-FOSPR) integrated with a CRISPR-Cas13a system for ultrasensitive and single-base-specific detection of
PCNSL-associated miRNA in aqueous humor. The BP nano-interface significantly enhances the surface plasmon resonance signal, while the CRISPR-Cas13a technology enables highly specific detection of miRNA, down to single nucleotide mismatches. This system achieves a detection limit as low as 21aM without the need for amplification and demonstrates robust performance in clinical samples. With its unparalleled sensitivity, specificity, label-free operation, and potential for portability, this biosensing platform offers transformative capabilities for early PCNSL diagnosis, prognosis, and treatment monitoring using minimally invasive liquid biopsy.
.
期刊介绍:
Reports on Progress in Physics is a highly selective journal with a mission to publish ground-breaking new research and authoritative invited reviews of the highest quality and significance across all areas of physics and related areas. Articles must be essential reading for specialists, and likely to be of broader multidisciplinary interest with the expectation for long-term scientific impact and influence on the current state and/or future direction of a field.