山羊妊娠前后卵巢组织microRNA动态表达谱

IF 2.9 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Yanyan Wang, Peipei He, Tianle Chao, Qing Li, Lu Zhang, Jingsen Liang, Ying Wei, Jiaqing Hu, Jianmin Wang
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引用次数: 0

摘要

本研究对JG山羊三个关键生殖阶段的卵巢组织进行了转录组测序——非妊娠期(8个月大,M8)、妊娠期(10个月大,M10)和产后期(12个月大,M12)。在所有文库中共鉴定出93个差异表达(DE) mirna,其中M8组与M10组数量最多(34个上调,47个下调;| >;1、罗斯福&;lt;0.05)。这些阶段特异性DE mirna聚集为四组,其靶基因主要富集于Wnt、JAK-STAT、PI3K-Akt、VEGF和类固醇生物合成等信号通路,是影响卵巢细胞活性、激素分泌、血管生成和胚胎发育的重要因素。实时荧光定量PCR验证表明,所选择的9个DE mirna的表达模式与测序数据一致。此外,通过对DE mirna和mrna的联合分析,构建了与卵巢生殖功能变化相关的共表达网络,最终突出了11个关键mirna。双荧光素酶报告基因检测进一步证实了核心miR-432-5p与CPT1B、miR-455-3p与PLXNB1之间的直接靶向关系。综上所述,本研究揭示了妊娠前期至产后卵巢miRNA表达谱的动态变化及其可能的分子机制,为miRNA在生殖调控中的作用提供了理论支持和关键分子靶点,对提高家畜生殖效率具有重要意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Dynamic microRNA expression profiles in goat ovarian tissues before and after pregnancy
This study performed transcriptome sequencing of ovarian tissues from JG goats at three key reproductive stages—non-pregnant (8 months old, M8), pregnant (10 months old, M10), and postpartum (12 months old, M12). A total of 93 differentially expressed (DE) miRNAs were identified across all libraries, with the M8 vs. M10 group having the largest number (34 upregulated and 47 downregulated; |log₂ fold change| > 1, FDR < 0.05). These stage-specific DE miRNAs were clustered into four groups, with their target genes primarily enriched in signaling pathways such as Wnt, JAK-STAT, PI3K-Akt, VEGF, and steroid biosynthesis, which are important factors influencing ovarian cell activity, hormone secretion, angiogenesis, and embryonic development. Real-time quantitative PCR verification showed that the expression patterns of the 9 selected DE miRNAs were consistent with the sequencing data. Additionally, through the joint analysis of DE miRNAs and mRNAs, a co-expression network associated with ovarian reproductive function changes was constructed, ultimately highlighting 11 key miRNAs. Dual-luciferase reporter assays further confirmed the direct targeting relationships between core miR-432-5p and CPT1B, as well as miR-455-3p and PLXNB1. In summary, this study revealed the dynamic changes in ovarian miRNA expression profiles from pre-pregnancy to postpartum and their potential molecular mechanisms, providing theoretical support and key molecular targets for miRNAs in reproductive regulation, with significant implications for improving reproductive efficiency in livestock.
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来源期刊
Journal of animal science
Journal of animal science 农林科学-奶制品与动物科学
CiteScore
4.80
自引率
12.10%
发文量
1589
审稿时长
3 months
期刊介绍: The Journal of Animal Science (JAS) is the premier journal for animal science and serves as the leading source of new knowledge and perspective in this area. JAS publishes more than 500 fully reviewed research articles, invited reviews, technical notes, and letters to the editor each year. Articles published in JAS encompass a broad range of research topics in animal production and fundamental aspects of genetics, nutrition, physiology, and preparation and utilization of animal products. Articles typically report research with beef cattle, companion animals, goats, horses, pigs, and sheep; however, studies involving other farm animals, aquatic and wildlife species, and laboratory animal species that address fundamental questions related to livestock and companion animal biology will be considered for publication.
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