Yanyan Wang, Peipei He, Tianle Chao, Qing Li, Lu Zhang, Jingsen Liang, Ying Wei, Jiaqing Hu, Jianmin Wang
{"title":"山羊妊娠前后卵巢组织microRNA动态表达谱","authors":"Yanyan Wang, Peipei He, Tianle Chao, Qing Li, Lu Zhang, Jingsen Liang, Ying Wei, Jiaqing Hu, Jianmin Wang","doi":"10.1093/jas/skaf187","DOIUrl":null,"url":null,"abstract":"This study performed transcriptome sequencing of ovarian tissues from JG goats at three key reproductive stages—non-pregnant (8 months old, M8), pregnant (10 months old, M10), and postpartum (12 months old, M12). A total of 93 differentially expressed (DE) miRNAs were identified across all libraries, with the M8 vs. M10 group having the largest number (34 upregulated and 47 downregulated; |log₂ fold change| > 1, FDR < 0.05). These stage-specific DE miRNAs were clustered into four groups, with their target genes primarily enriched in signaling pathways such as Wnt, JAK-STAT, PI3K-Akt, VEGF, and steroid biosynthesis, which are important factors influencing ovarian cell activity, hormone secretion, angiogenesis, and embryonic development. Real-time quantitative PCR verification showed that the expression patterns of the 9 selected DE miRNAs were consistent with the sequencing data. Additionally, through the joint analysis of DE miRNAs and mRNAs, a co-expression network associated with ovarian reproductive function changes was constructed, ultimately highlighting 11 key miRNAs. Dual-luciferase reporter assays further confirmed the direct targeting relationships between core miR-432-5p and CPT1B, as well as miR-455-3p and PLXNB1. In summary, this study revealed the dynamic changes in ovarian miRNA expression profiles from pre-pregnancy to postpartum and their potential molecular mechanisms, providing theoretical support and key molecular targets for miRNAs in reproductive regulation, with significant implications for improving reproductive efficiency in livestock.","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":"9 1","pages":""},"PeriodicalIF":2.9000,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Dynamic microRNA expression profiles in goat ovarian tissues before and after pregnancy\",\"authors\":\"Yanyan Wang, Peipei He, Tianle Chao, Qing Li, Lu Zhang, Jingsen Liang, Ying Wei, Jiaqing Hu, Jianmin Wang\",\"doi\":\"10.1093/jas/skaf187\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This study performed transcriptome sequencing of ovarian tissues from JG goats at three key reproductive stages—non-pregnant (8 months old, M8), pregnant (10 months old, M10), and postpartum (12 months old, M12). A total of 93 differentially expressed (DE) miRNAs were identified across all libraries, with the M8 vs. M10 group having the largest number (34 upregulated and 47 downregulated; |log₂ fold change| > 1, FDR < 0.05). These stage-specific DE miRNAs were clustered into four groups, with their target genes primarily enriched in signaling pathways such as Wnt, JAK-STAT, PI3K-Akt, VEGF, and steroid biosynthesis, which are important factors influencing ovarian cell activity, hormone secretion, angiogenesis, and embryonic development. Real-time quantitative PCR verification showed that the expression patterns of the 9 selected DE miRNAs were consistent with the sequencing data. Additionally, through the joint analysis of DE miRNAs and mRNAs, a co-expression network associated with ovarian reproductive function changes was constructed, ultimately highlighting 11 key miRNAs. Dual-luciferase reporter assays further confirmed the direct targeting relationships between core miR-432-5p and CPT1B, as well as miR-455-3p and PLXNB1. In summary, this study revealed the dynamic changes in ovarian miRNA expression profiles from pre-pregnancy to postpartum and their potential molecular mechanisms, providing theoretical support and key molecular targets for miRNAs in reproductive regulation, with significant implications for improving reproductive efficiency in livestock.\",\"PeriodicalId\":14895,\"journal\":{\"name\":\"Journal of animal science\",\"volume\":\"9 1\",\"pages\":\"\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2025-07-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of animal science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1093/jas/skaf187\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of animal science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1093/jas/skaf187","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
Dynamic microRNA expression profiles in goat ovarian tissues before and after pregnancy
This study performed transcriptome sequencing of ovarian tissues from JG goats at three key reproductive stages—non-pregnant (8 months old, M8), pregnant (10 months old, M10), and postpartum (12 months old, M12). A total of 93 differentially expressed (DE) miRNAs were identified across all libraries, with the M8 vs. M10 group having the largest number (34 upregulated and 47 downregulated; |log₂ fold change| > 1, FDR < 0.05). These stage-specific DE miRNAs were clustered into four groups, with their target genes primarily enriched in signaling pathways such as Wnt, JAK-STAT, PI3K-Akt, VEGF, and steroid biosynthesis, which are important factors influencing ovarian cell activity, hormone secretion, angiogenesis, and embryonic development. Real-time quantitative PCR verification showed that the expression patterns of the 9 selected DE miRNAs were consistent with the sequencing data. Additionally, through the joint analysis of DE miRNAs and mRNAs, a co-expression network associated with ovarian reproductive function changes was constructed, ultimately highlighting 11 key miRNAs. Dual-luciferase reporter assays further confirmed the direct targeting relationships between core miR-432-5p and CPT1B, as well as miR-455-3p and PLXNB1. In summary, this study revealed the dynamic changes in ovarian miRNA expression profiles from pre-pregnancy to postpartum and their potential molecular mechanisms, providing theoretical support and key molecular targets for miRNAs in reproductive regulation, with significant implications for improving reproductive efficiency in livestock.
期刊介绍:
The Journal of Animal Science (JAS) is the premier journal for animal science and serves as the leading source of new knowledge and perspective in this area. JAS publishes more than 500 fully reviewed research articles, invited reviews, technical notes, and letters to the editor each year.
Articles published in JAS encompass a broad range of research topics in animal production and fundamental aspects of genetics, nutrition, physiology, and preparation and utilization of animal products. Articles typically report research with beef cattle, companion animals, goats, horses, pigs, and sheep; however, studies involving other farm animals, aquatic and wildlife species, and laboratory animal species that address fundamental questions related to livestock and companion animal biology will be considered for publication.