探索线粒体光毒性：机制、测量和缓解。

IF 3.3 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochimie Pub Date : 2025-07-07 DOI:10.1016/j.biochi.2025.07.008

Bowen Liu , Kai Chen , Yubing Han

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引用次数: 0

摘要

由光暴露引起的光毒性是荧光显微镜研究的主要挑战，特别是在研究线粒体时。这些细胞器对成像过程中产生的活性氧（ROS）的损伤很敏感。本文综述了光毒性的基本原理，重点介绍了光毒性对线粒体功能和结构的影响。它还探讨了减轻损伤的策略，包括优化的成像技术和专门的探针，并考虑了光毒性用于治疗目的的情况。强调需要先进的成像方法来平衡损伤预防和线粒体研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Exploring mitochondrial phototoxicity: Mechanisms, measurement, and mitigation

Phototoxicity, induced by light exposure, presents a major challenge in fluorescence microscopy, particularly when studying mitochondria. These organelles are sensitive to damage from reactive oxygen species (ROS) generated during imaging. This review discusses the basic principles of phototoxicity, focusing primarily on the effects of phototoxicity on mitochondrial function and structure. It also explores strategies to mitigate damage, including optimized imaging techniques and specialized probes, and considers cases where phototoxicity is utilized for therapeutic purposes. The need for advanced imaging methods to balance damage prevention and mitochondrial research is emphasized.

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来源期刊

Biochimie 生物-生化与分子生物学

CiteScore

7.20

自引率

2.60%

发文量

219

审稿时长

40 days

期刊介绍： Biochimie publishes original research articles, short communications, review articles, graphical reviews, mini-reviews, and hypotheses in the broad areas of biology, including biochemistry, enzymology, molecular and cell biology, metabolic regulation, genetics, immunology, microbiology, structural biology, genomics, proteomics, and molecular mechanisms of disease. Biochimie publishes exclusively in English. Articles are subject to peer review, and must satisfy the requirements of originality, high scientific integrity and general interest to a broad range of readers. Submissions that are judged to be of sound scientific and technical quality but do not fully satisfy the requirements for publication in Biochimie may benefit from a transfer service to a more suitable journal within the same subject area.