Tobias Lindner, Adrian Konstantin Luyken, Chris Lappe, Oliver Stachs, Thoralf Niendorf, Matthias Lütgens, Stefan Polei, Brigitte Vollmar, Andreas Buettner, Sönke Langner, Marc-André Weber, Ebba Beller
{"title":"23Na MRI定量测定浸泡在盐水和淡水途中猪眼内钠含量。","authors":"Tobias Lindner, Adrian Konstantin Luyken, Chris Lappe, Oliver Stachs, Thoralf Niendorf, Matthias Lütgens, Stefan Polei, Brigitte Vollmar, Andreas Buettner, Sönke Langner, Marc-André Weber, Ebba Beller","doi":"10.1186/s41747-025-00605-x","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Differentiation between saltwater and freshwater immersion as well as estimating the corpse's time in water can be challenging. We aimed to establish and examine the feasibility of a novel approach based on sodium magnetic resonance imaging (<sup>23</sup>Na MRI) of the eye to facilitate noninvasive sodium quantification.</p><p><strong>Methods: </strong>Enucleated porcine eyes were immersed in NaCl 0.9%, NaCl 3.0%, NaCl 5.85%, distilled water (DW) or lake water (LW) at different time intervals, followed by <sup>23</sup>Na 7-T MRI sodium quantification.</p><p><strong>Results: </strong>After 6 h of immersion, a significant difference in vitreous body (VB) sodium concentration was found for NaCl 5.85% versus DW or LW (p ≤ 0.019). After 24 and 48 h of immersion, a significant difference in VB sodium concentration was found for NaCl 5.85% versus DW, LW, NaCl 3.0% or NaCl 0.9%, as well as for NaCl 3.0% versus DW, LW or NaCl 0.9% (p ≤ 0.001). After 24 h of immersion, lens sodium concentration showed a significant difference for NaCl 5.85% versus DW, LW, NaCl 3.0% or NaCl 0.9% (p ≤ 0.009); after 48 h of immersion, for NaCl 5.85% versus DW, LW, NaCl 3.0% or NaCl 0.9% (p ≤ 0.001), as well as for NaCl 3.0% versus DW, LW or NaCl 0.9% (p ≤ 0.007). For VB, sodium concentration changes over immersion time, and exponential curves were fitted to the data.</p><p><strong>Conclusion: </strong>Using <sup>23</sup>Na MRI in ex vivo porcine eyes with different immersion times in various saltwater concentrations and freshwater equivalents allowed noninvasive quantification of VB and lens sodium concentrations.</p><p><strong>Relevance statement: </strong>Although not a substitute for autopsy, <sup>23</sup>Na MRI assessment of VB and lens sodium concentrations may provide biochemical support in suspected drowning, especially in cases where an internal examination of the body is not authorized or where objections to autopsy are upheld.</p><p><strong>Key points: </strong>Postmortem porcine eyes with different immersion times in saltwater and freshwater. Noninvasive quantification of vitreous body and lens sodium concentrations with <sup>23</sup>Na MRI. Exponential time course of vitreous body sodium concentration in saltwater and freshwater.</p>","PeriodicalId":36926,"journal":{"name":"European Radiology Experimental","volume":"9 1","pages":"66"},"PeriodicalIF":3.7000,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12240899/pdf/","citationCount":"0","resultStr":"{\"title\":\"<sup>23</sup>Na MRI quantification of sodium content in porcine eyes after immersion in saltwater and freshwater en route to time in water estimation.\",\"authors\":\"Tobias Lindner, Adrian Konstantin Luyken, Chris Lappe, Oliver Stachs, Thoralf Niendorf, Matthias Lütgens, Stefan Polei, Brigitte Vollmar, Andreas Buettner, Sönke Langner, Marc-André Weber, Ebba Beller\",\"doi\":\"10.1186/s41747-025-00605-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Differentiation between saltwater and freshwater immersion as well as estimating the corpse's time in water can be challenging. We aimed to establish and examine the feasibility of a novel approach based on sodium magnetic resonance imaging (<sup>23</sup>Na MRI) of the eye to facilitate noninvasive sodium quantification.</p><p><strong>Methods: </strong>Enucleated porcine eyes were immersed in NaCl 0.9%, NaCl 3.0%, NaCl 5.85%, distilled water (DW) or lake water (LW) at different time intervals, followed by <sup>23</sup>Na 7-T MRI sodium quantification.</p><p><strong>Results: </strong>After 6 h of immersion, a significant difference in vitreous body (VB) sodium concentration was found for NaCl 5.85% versus DW or LW (p ≤ 0.019). After 24 and 48 h of immersion, a significant difference in VB sodium concentration was found for NaCl 5.85% versus DW, LW, NaCl 3.0% or NaCl 0.9%, as well as for NaCl 3.0% versus DW, LW or NaCl 0.9% (p ≤ 0.001). After 24 h of immersion, lens sodium concentration showed a significant difference for NaCl 5.85% versus DW, LW, NaCl 3.0% or NaCl 0.9% (p ≤ 0.009); after 48 h of immersion, for NaCl 5.85% versus DW, LW, NaCl 3.0% or NaCl 0.9% (p ≤ 0.001), as well as for NaCl 3.0% versus DW, LW or NaCl 0.9% (p ≤ 0.007). For VB, sodium concentration changes over immersion time, and exponential curves were fitted to the data.</p><p><strong>Conclusion: </strong>Using <sup>23</sup>Na MRI in ex vivo porcine eyes with different immersion times in various saltwater concentrations and freshwater equivalents allowed noninvasive quantification of VB and lens sodium concentrations.</p><p><strong>Relevance statement: </strong>Although not a substitute for autopsy, <sup>23</sup>Na MRI assessment of VB and lens sodium concentrations may provide biochemical support in suspected drowning, especially in cases where an internal examination of the body is not authorized or where objections to autopsy are upheld.</p><p><strong>Key points: </strong>Postmortem porcine eyes with different immersion times in saltwater and freshwater. Noninvasive quantification of vitreous body and lens sodium concentrations with <sup>23</sup>Na MRI. Exponential time course of vitreous body sodium concentration in saltwater and freshwater.</p>\",\"PeriodicalId\":36926,\"journal\":{\"name\":\"European Radiology Experimental\",\"volume\":\"9 1\",\"pages\":\"66\"},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2025-07-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12240899/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"European Radiology Experimental\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1186/s41747-025-00605-x\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"European Radiology Experimental","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/s41747-025-00605-x","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING","Score":null,"Total":0}
23Na MRI quantification of sodium content in porcine eyes after immersion in saltwater and freshwater en route to time in water estimation.
Background: Differentiation between saltwater and freshwater immersion as well as estimating the corpse's time in water can be challenging. We aimed to establish and examine the feasibility of a novel approach based on sodium magnetic resonance imaging (23Na MRI) of the eye to facilitate noninvasive sodium quantification.
Methods: Enucleated porcine eyes were immersed in NaCl 0.9%, NaCl 3.0%, NaCl 5.85%, distilled water (DW) or lake water (LW) at different time intervals, followed by 23Na 7-T MRI sodium quantification.
Results: After 6 h of immersion, a significant difference in vitreous body (VB) sodium concentration was found for NaCl 5.85% versus DW or LW (p ≤ 0.019). After 24 and 48 h of immersion, a significant difference in VB sodium concentration was found for NaCl 5.85% versus DW, LW, NaCl 3.0% or NaCl 0.9%, as well as for NaCl 3.0% versus DW, LW or NaCl 0.9% (p ≤ 0.001). After 24 h of immersion, lens sodium concentration showed a significant difference for NaCl 5.85% versus DW, LW, NaCl 3.0% or NaCl 0.9% (p ≤ 0.009); after 48 h of immersion, for NaCl 5.85% versus DW, LW, NaCl 3.0% or NaCl 0.9% (p ≤ 0.001), as well as for NaCl 3.0% versus DW, LW or NaCl 0.9% (p ≤ 0.007). For VB, sodium concentration changes over immersion time, and exponential curves were fitted to the data.
Conclusion: Using 23Na MRI in ex vivo porcine eyes with different immersion times in various saltwater concentrations and freshwater equivalents allowed noninvasive quantification of VB and lens sodium concentrations.
Relevance statement: Although not a substitute for autopsy, 23Na MRI assessment of VB and lens sodium concentrations may provide biochemical support in suspected drowning, especially in cases where an internal examination of the body is not authorized or where objections to autopsy are upheld.
Key points: Postmortem porcine eyes with different immersion times in saltwater and freshwater. Noninvasive quantification of vitreous body and lens sodium concentrations with 23Na MRI. Exponential time course of vitreous body sodium concentration in saltwater and freshwater.
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