甲氨蝶呤负载肌醇-6磷酸交联壳聚糖纳米颗粒的制备及对乳腺癌的抗癌作用评价。

IF 4.1 Q2 PHARMACOLOGY & PHARMACY
Advanced pharmaceutical bulletin Pub Date : 2025-03-08 eCollection Date: 2025-04-01 DOI:10.34172/apb.43661
Masoud Farshbaf, Nasrin Gobakhlou, Muhammad Sarfraz, Javid Shahbazi-Mojarrad, Mohammad Feyzizadeh, Hamed Hamishehkar, Parvin Zakeri-Milani, Hadi Valizadeh
{"title":"甲氨蝶呤负载肌醇-6磷酸交联壳聚糖纳米颗粒的制备及对乳腺癌的抗癌作用评价。","authors":"Masoud Farshbaf, Nasrin Gobakhlou, Muhammad Sarfraz, Javid Shahbazi-Mojarrad, Mohammad Feyzizadeh, Hamed Hamishehkar, Parvin Zakeri-Milani, Hadi Valizadeh","doi":"10.34172/apb.43661","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>Chitosan nanoparticles (CNs) have directed considerable research efforts towards developing biocompatible, biodegradable, inexpensive and efficient particulate drug delivery systems.</p><p><strong>Methods: </strong>In the present investigation, we utilized green and safe inositol hexaphosphate (InsP6) as a physical cross-linker to obtain CNs (<sup>InsP6</sup>CNs) and compared their size, zeta potential and cell uptake ability with the CNs cross-linked with tripolyphosphate (TPP) as a commonly used cross-linker (<sup>TPP</sup>CNs). Methotrexate (MTX) as the model drug was physically incorporated within the both types of CNs (<sup>InsP6</sup>CNs<sub>MTX</sub> and <sup>TPP</sup>CNs<sub>MTX</sub>) and their time-dependent anti-cancer behavior was evaluated on MCF-7 cell line.</p><p><strong>Results: </strong>Compared to <sup>TPP</sup>CNs, <sup>InsP6</sup>CNs were bigger in hydrodynamic diameter and showed far different zeta potential value. The MTX encapsulation efficiency was much higher for <sup>InsP6</sup>CNs<sub>MTX</sub> than that of <sup>TPP</sup>CNs<sub>MTX</sub>. <sup>InsP6</sup>CNs and <sup>TPP</sup>CNs showed similar <i>in vitro</i> cell uptake behavior, examined on MCF-7 cell line. Furthermore, after 24 h, <sup>InsP6</sup>CNs<sub>MTX</sub> had the most <i>in vitro</i> antitumor effect on the MCF-7 cells, compared to free MTX and <sup>TPP</sup>CNs<sub>MTX</sub>.</p><p><strong>Conclusion: </strong>Consequently, InsP6 can be presented as an accessible and cost-effective member of physical cross-linkers to prepare efficient CNs as drug delivery systems.</p>","PeriodicalId":7256,"journal":{"name":"Advanced pharmaceutical bulletin","volume":"15 1","pages":"217-222"},"PeriodicalIF":4.1000,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12235370/pdf/","citationCount":"0","resultStr":"{\"title\":\"Preparation and Anti-cancer Evaluation of Methotrexate-loaded Inositol-6 Phosphate Cross-linked Chitosan Nanoparticles on Breast Cancer.\",\"authors\":\"Masoud Farshbaf, Nasrin Gobakhlou, Muhammad Sarfraz, Javid Shahbazi-Mojarrad, Mohammad Feyzizadeh, Hamed Hamishehkar, Parvin Zakeri-Milani, Hadi Valizadeh\",\"doi\":\"10.34172/apb.43661\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>Chitosan nanoparticles (CNs) have directed considerable research efforts towards developing biocompatible, biodegradable, inexpensive and efficient particulate drug delivery systems.</p><p><strong>Methods: </strong>In the present investigation, we utilized green and safe inositol hexaphosphate (InsP6) as a physical cross-linker to obtain CNs (<sup>InsP6</sup>CNs) and compared their size, zeta potential and cell uptake ability with the CNs cross-linked with tripolyphosphate (TPP) as a commonly used cross-linker (<sup>TPP</sup>CNs). Methotrexate (MTX) as the model drug was physically incorporated within the both types of CNs (<sup>InsP6</sup>CNs<sub>MTX</sub> and <sup>TPP</sup>CNs<sub>MTX</sub>) and their time-dependent anti-cancer behavior was evaluated on MCF-7 cell line.</p><p><strong>Results: </strong>Compared to <sup>TPP</sup>CNs, <sup>InsP6</sup>CNs were bigger in hydrodynamic diameter and showed far different zeta potential value. The MTX encapsulation efficiency was much higher for <sup>InsP6</sup>CNs<sub>MTX</sub> than that of <sup>TPP</sup>CNs<sub>MTX</sub>. <sup>InsP6</sup>CNs and <sup>TPP</sup>CNs showed similar <i>in vitro</i> cell uptake behavior, examined on MCF-7 cell line. Furthermore, after 24 h, <sup>InsP6</sup>CNs<sub>MTX</sub> had the most <i>in vitro</i> antitumor effect on the MCF-7 cells, compared to free MTX and <sup>TPP</sup>CNs<sub>MTX</sub>.</p><p><strong>Conclusion: </strong>Consequently, InsP6 can be presented as an accessible and cost-effective member of physical cross-linkers to prepare efficient CNs as drug delivery systems.</p>\",\"PeriodicalId\":7256,\"journal\":{\"name\":\"Advanced pharmaceutical bulletin\",\"volume\":\"15 1\",\"pages\":\"217-222\"},\"PeriodicalIF\":4.1000,\"publicationDate\":\"2025-03-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12235370/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Advanced pharmaceutical bulletin\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.34172/apb.43661\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/4/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"PHARMACOLOGY & PHARMACY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advanced pharmaceutical bulletin","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.34172/apb.43661","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/4/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
引用次数: 0

摘要

目的:壳聚糖纳米颗粒(CNs)在开发生物相容性、生物可降解、廉价高效的颗粒给药系统方面具有重要的研究意义。方法:本研究以绿色安全的六磷酸肌醇(InsP6)作为物理交联剂制备CNs (InsP6CNs),并与常用的三聚磷酸(TPP)交联CNs (TPPCNs)进行尺寸、zeta电位和细胞摄取能力的比较。将甲氨蝶呤(MTX)作为模型药物物理掺入两种类型的cnns (InsP6CNsMTX和TPPCNsMTX)中,并在MCF-7细胞系上评估其时间依赖性抗癌行为。结果:与TPPCNs相比,InsP6CNs的水动力直径更大,zeta电位值差异较大。与TPPCNsMTX相比,InsP6CNsMTX的MTX封装效率更高。在MCF-7细胞系上检测了InsP6CNs和TPPCNs的体外细胞摄取行为。此外,24 h后,与游离MTX和TPPCNsMTX相比,InsP6CNsMTX对MCF-7细胞的体外抗肿瘤作用最大。结论:因此,InsP6可以作为一种可获得且具有成本效益的物理交联剂成员来制备高效的神经网络作为药物传递系统。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Preparation and Anti-cancer Evaluation of Methotrexate-loaded Inositol-6 Phosphate Cross-linked Chitosan Nanoparticles on Breast Cancer.

Purpose: Chitosan nanoparticles (CNs) have directed considerable research efforts towards developing biocompatible, biodegradable, inexpensive and efficient particulate drug delivery systems.

Methods: In the present investigation, we utilized green and safe inositol hexaphosphate (InsP6) as a physical cross-linker to obtain CNs (InsP6CNs) and compared their size, zeta potential and cell uptake ability with the CNs cross-linked with tripolyphosphate (TPP) as a commonly used cross-linker (TPPCNs). Methotrexate (MTX) as the model drug was physically incorporated within the both types of CNs (InsP6CNsMTX and TPPCNsMTX) and their time-dependent anti-cancer behavior was evaluated on MCF-7 cell line.

Results: Compared to TPPCNs, InsP6CNs were bigger in hydrodynamic diameter and showed far different zeta potential value. The MTX encapsulation efficiency was much higher for InsP6CNsMTX than that of TPPCNsMTX. InsP6CNs and TPPCNs showed similar in vitro cell uptake behavior, examined on MCF-7 cell line. Furthermore, after 24 h, InsP6CNsMTX had the most in vitro antitumor effect on the MCF-7 cells, compared to free MTX and TPPCNsMTX.

Conclusion: Consequently, InsP6 can be presented as an accessible and cost-effective member of physical cross-linkers to prepare efficient CNs as drug delivery systems.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Advanced pharmaceutical bulletin
Advanced pharmaceutical bulletin PHARMACOLOGY & PHARMACY-
CiteScore
6.80
自引率
2.80%
发文量
51
审稿时长
12 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信