在COVID-19大流行期间，共同产生KPC和NDM的肺炎克雷伯菌ST307质粒多样性恢复

IF 2.6 4区医学 Q3 INFECTIOUS DISEASES

Infection Genetics and Evolution Pub Date : 2025-07-08 DOI:10.1016/j.meegid.2025.105795

Sonia Alejandra Gomez , Florencia Martino , María Belén Sanz , Jenny Escalante , Juan Manuel de Mendieta , Celeste Lucero , Paola Ceriana , Fernando Pasteran , Alejandra Corso , María Soledad Ramirez , Diego Faccone

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引用次数: 0

摘要

在2019冠状病毒病期间，阿根廷产碳青霉烯酶肺炎克雷伯菌的流行率显著上升，从2019年的20%上升到2021年的30%。此外，联合产生KPC和NDM的肺炎克雷伯菌ST307显著增加。我们的目的是揭示6株共同产生KPC和NDM的肺炎克雷伯菌ST307分离株的质粒的遗传结构，以及这两种碳青霉烯酶在细胞中的表达水平。采集时间为2020年11月至2021年9月，筛选结果如下：(i)等位基因变异组合多样性：blaKPC-2 + blaNDM-1 （n = 2）、blaKPC-2 + blaNDM-5 （n = 2）、blaKPC-3 + blaNDM-1 (n = 2)；（ii）不同的医院和司法管辖区；（iii） Xba-I-PFGE的脉冲型差异。采用全基因组测序（Illumina NextSeq 550和GridION）和实时荧光定量pcr进行转录分析。分离株携带2 ~ 4个大小为3.7 ~ 250kb的质粒。共鉴定出20个具有9个复制子的质粒，包括3个不同的杂交复制子组合。blaKPC-2在三个质粒骨干中发现[IncM1， IncFIB(pQil)/IncFII(K)和IncFIB/H1B]，而其余的则与单一质粒类型相关：blaNDM-1 (IncC), blaNDM-5 （IncFIB/H1B）和blaKPC-3 （IncR）。KPC-2的基础表达水平明显高于NDM-1/5。我们的研究结果表明，NDM和KPC在肺炎克雷伯菌ST307中的传播是通过不同的质粒驱动的，突出了这种高风险克隆获得、维持和传播多种碳青霉烯酶的能力。

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Plasmid diversity in Klebsiella pneumoniae ST307 co-producing KPC plus NDM recovered during the COVID-19 pandemic

The prevalence of carbapenemase-producing Klebsiella pneumoniae increased significantly during COVID-19 in Argentina, rising from 20 % in 2019 to 30 % in 2021. Additionally, there was a notable increase of K. pneumoniae ST307 co-producing KPC and NDM. We aimed to reveal the genetic structure of the plasmids harbored in six isolates of K. pneumoniae ST307 co-producing KPC and NDM and to expression levels of both carbapenemases in a cell. The isolates were collected between November 2020 and September 2021 and were selected according to: (i) diversity of allelic variant combinations: bla_KPC-2 plus bla_NDM-1 (n = 2), bla_KPC-2 plus bla_NDM-5 (n = 2), bla_KPC-3 plus bla_NDM-1 (n = 2); (ii) different hospitals and jurisdictions; and (iii) differences in pulsotype by Xba-I-PFGE. The isolates were studied by whole genome sequencing (Illumina NextSeq 550 and with GridION) and with a transcriptional analysis using quantitative real time-PCR. The isolates carried between two and four plasmids with sizes from 3.7 to 250 Kb. Twenty plasmids with nine replicons were identified including three distinct hybrid replicon combinations. bla_KPC-2 was found in three plasmid backbones [IncM1, IncFIB(pQil)/IncFII(K), and IncFIB/H1B] while the rest were associated with a single plasmid type: bla_NDM-1 (IncC), bla_NDM-5 (IncFIB/H1B) and bla_KPC-3 (IncR). The basal expression level of KPC-2 was statistically higher than that of NDM-1/5. Our results show that the dissemination of NDM and KPC in K. pneumoniae ST307 was driven through diverse plasmids, highlighting the capacity of this high-risk clone to acquire, maintain and spread multiple carbapenemases.

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来源期刊

Infection Genetics and Evolution 医学-传染病学

CiteScore

8.40

自引率

0.00%

发文量

215

审稿时长

82 days

期刊介绍： (aka Journal of Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases -- MEEGID) Infectious diseases constitute one of the main challenges to medical science in the coming century. The impressive development of molecular megatechnologies and of bioinformatics have greatly increased our knowledge of the evolution, transmission and pathogenicity of infectious diseases. Research has shown that host susceptibility to many infectious diseases has a genetic basis. Furthermore, much is now known on the molecular epidemiology, evolution and virulence of pathogenic agents, as well as their resistance to drugs, vaccines, and antibiotics. Equally, research on the genetics of disease vectors has greatly improved our understanding of their systematics, has increased our capacity to identify target populations for control or intervention, and has provided detailed information on the mechanisms of insecticide resistance. However, the genetics and evolutionary biology of hosts, pathogens and vectors have tended to develop as three separate fields of research. This artificial compartmentalisation is of concern due to our growing appreciation of the strong co-evolutionary interactions among hosts, pathogens and vectors. Infection, Genetics and Evolution and its companion congress [MEEGID](http://www.meegidconference.com/) (for Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases) are the main forum acting for the cross-fertilization between evolutionary science and biomedical research on infectious diseases. Infection, Genetics and Evolution is the only journal that welcomes articles dealing with the genetics and evolutionary biology of hosts, pathogens and vectors, and coevolution processes among them in relation to infection and disease manifestation. All infectious models enter the scope of the journal, including pathogens of humans, animals and plants, either parasites, fungi, bacteria, viruses or prions. The journal welcomes articles dealing with genetics, population genetics, genomics, postgenomics, gene expression, evolutionary biology, population dynamics, mathematical modeling and bioinformatics. We also provide many author benefits, such as free PDFs, a liberal copyright policy, special discounts on Elsevier publications and much more. Please click here for more information on our author services .