Rebeka Frueholz , Clemens Habermaier , Sabine Spiess , Ludwig Birklbauer , Georg M. Guebitz , Marianne Haberbauer
{"title":"利用化化嗜硫微生物酸化硫杆菌从铸造厂粉尘中提取锌","authors":"Rebeka Frueholz , Clemens Habermaier , Sabine Spiess , Ludwig Birklbauer , Georg M. Guebitz , Marianne Haberbauer","doi":"10.1016/j.bej.2025.109859","DOIUrl":null,"url":null,"abstract":"<div><div>The continuous generation of steel by-products calls for innovative recycling solutions in order to strive towards a circular economy. In this study, the bioleaching potential of the chemolithotrophic microorganism <em>A. thiooxidans</em> was evaluated to extract unwanted Zn from the steel by-product cast-house dust (CHD) while specifically retaining Fe within the dust matrix to enable on-site recycling. A fed-batch stirred tank reactor was employed, and process stability was assessed through a series of replicates at a CHD concentration of 125 g L⁻¹ . Two distinct aerations 0.42 vvm and 0.50 vvm were examined during the course of this investigation. After 14 days, a maximum of 75 ± 5 % Zn underwent solubilization while only 9 ± 2 % of Fe was solubilized. However, after 7 days the majority of Zn was extracted resulting in an average leaching efficiency of 68 ± 2 % for Zn and 5 ± 2 % for Fe. Although increasing the aeration rate did not significantly improve the <em>k<sub>L</sub>a</em> value, it slightly enhanced O₂ availability, highlighting the need for further optimization of the aeration setup.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"222 ","pages":"Article 109859"},"PeriodicalIF":3.7000,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Zinc extraction from cast-house dust using the chemolithotrophic microorganism Acidithiobacillus thiooxidans\",\"authors\":\"Rebeka Frueholz , Clemens Habermaier , Sabine Spiess , Ludwig Birklbauer , Georg M. Guebitz , Marianne Haberbauer\",\"doi\":\"10.1016/j.bej.2025.109859\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The continuous generation of steel by-products calls for innovative recycling solutions in order to strive towards a circular economy. In this study, the bioleaching potential of the chemolithotrophic microorganism <em>A. thiooxidans</em> was evaluated to extract unwanted Zn from the steel by-product cast-house dust (CHD) while specifically retaining Fe within the dust matrix to enable on-site recycling. A fed-batch stirred tank reactor was employed, and process stability was assessed through a series of replicates at a CHD concentration of 125 g L⁻¹ . Two distinct aerations 0.42 vvm and 0.50 vvm were examined during the course of this investigation. After 14 days, a maximum of 75 ± 5 % Zn underwent solubilization while only 9 ± 2 % of Fe was solubilized. However, after 7 days the majority of Zn was extracted resulting in an average leaching efficiency of 68 ± 2 % for Zn and 5 ± 2 % for Fe. Although increasing the aeration rate did not significantly improve the <em>k<sub>L</sub>a</em> value, it slightly enhanced O₂ availability, highlighting the need for further optimization of the aeration setup.</div></div>\",\"PeriodicalId\":8766,\"journal\":{\"name\":\"Biochemical Engineering Journal\",\"volume\":\"222 \",\"pages\":\"Article 109859\"},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2025-07-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochemical Engineering Journal\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1369703X25002335\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemical Engineering Journal","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1369703X25002335","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Zinc extraction from cast-house dust using the chemolithotrophic microorganism Acidithiobacillus thiooxidans
The continuous generation of steel by-products calls for innovative recycling solutions in order to strive towards a circular economy. In this study, the bioleaching potential of the chemolithotrophic microorganism A. thiooxidans was evaluated to extract unwanted Zn from the steel by-product cast-house dust (CHD) while specifically retaining Fe within the dust matrix to enable on-site recycling. A fed-batch stirred tank reactor was employed, and process stability was assessed through a series of replicates at a CHD concentration of 125 g L⁻¹ . Two distinct aerations 0.42 vvm and 0.50 vvm were examined during the course of this investigation. After 14 days, a maximum of 75 ± 5 % Zn underwent solubilization while only 9 ± 2 % of Fe was solubilized. However, after 7 days the majority of Zn was extracted resulting in an average leaching efficiency of 68 ± 2 % for Zn and 5 ± 2 % for Fe. Although increasing the aeration rate did not significantly improve the kLa value, it slightly enhanced O₂ availability, highlighting the need for further optimization of the aeration setup.
期刊介绍:
The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology.
The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields:
Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics
Biosensors and Biodevices including biofabrication and novel fuel cell development
Bioseparations including scale-up and protein refolding/renaturation
Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells
Bioreactor Systems including characterization, optimization and scale-up
Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization
Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals
Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release
Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites
Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation
Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis
Protein Engineering including enzyme engineering and directed evolution.