{"title":"在daf-16突变体daer幼虫中，Hbl-1不会导致成体细胞命运标记的错误表达。","authors":"Matthew J Wirick, Xantha Karp","doi":"10.17912/micropub.biology.001662","DOIUrl":null,"url":null,"abstract":"<p><p>In <i>Caenorhabditis elegans</i> dauer larvae, the FOXO ortholog, <i>daf-16</i> , opposes the expression of the <i>col-19p::gfp</i> adult cell fate marker in the lateral hypodermis. <i>daf-16</i> acts in part via <i>lin-41 ,</i> a heterochronic gene that promotes larval seam cell fate during non-dauer development. Here, we show that a different heterochronic gene, <i>hbl-1</i> , does not function downstream of <i>daf-16</i> to regulate <i>col-19p::gfp</i> expression during dauer. A gain-of-function <i>hbl-1</i> allele did not suppress ectopic <i>col-19p::gfp</i> expression in <i>daf-16 (0)</i> dauer larvae, and HBL-1 protein was not detectable in control or <i>daf-16 (0)</i> dauer larvae.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12235446/pdf/","citationCount":"0","resultStr":"{\"title\":\"<i>hbl-1</i> does not contribute to the misexpression of an adult cell fate marker in <i>daf-16</i> mutant dauer larvae.\",\"authors\":\"Matthew J Wirick, Xantha Karp\",\"doi\":\"10.17912/micropub.biology.001662\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>In <i>Caenorhabditis elegans</i> dauer larvae, the FOXO ortholog, <i>daf-16</i> , opposes the expression of the <i>col-19p::gfp</i> adult cell fate marker in the lateral hypodermis. <i>daf-16</i> acts in part via <i>lin-41 ,</i> a heterochronic gene that promotes larval seam cell fate during non-dauer development. Here, we show that a different heterochronic gene, <i>hbl-1</i> , does not function downstream of <i>daf-16</i> to regulate <i>col-19p::gfp</i> expression during dauer. A gain-of-function <i>hbl-1</i> allele did not suppress ectopic <i>col-19p::gfp</i> expression in <i>daf-16 (0)</i> dauer larvae, and HBL-1 protein was not detectable in control or <i>daf-16 (0)</i> dauer larvae.</p>\",\"PeriodicalId\":74192,\"journal\":{\"name\":\"microPublication biology\",\"volume\":\"2025 \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-06-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12235446/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"microPublication biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.17912/micropub.biology.001662\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"microPublication biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17912/micropub.biology.001662","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
hbl-1 does not contribute to the misexpression of an adult cell fate marker in daf-16 mutant dauer larvae.
In Caenorhabditis elegans dauer larvae, the FOXO ortholog, daf-16 , opposes the expression of the col-19p::gfp adult cell fate marker in the lateral hypodermis. daf-16 acts in part via lin-41 , a heterochronic gene that promotes larval seam cell fate during non-dauer development. Here, we show that a different heterochronic gene, hbl-1 , does not function downstream of daf-16 to regulate col-19p::gfp expression during dauer. A gain-of-function hbl-1 allele did not suppress ectopic col-19p::gfp expression in daf-16 (0) dauer larvae, and HBL-1 protein was not detectable in control or daf-16 (0) dauer larvae.
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