微量营养素补充影响健康成人肠道微生物组的组成和饮食起源功能

IF 7.4 2区医学 Q1 NUTRITION & DIETETICS

Clinical nutrition Pub Date : 2025-06-25 DOI:10.1016/j.clnu.2025.06.020

Shona Mckirdy , Athanasios Koutsos , Ben Nichols , Maris Anderson , Sumet Dhami , Chandrama Roy Chowdhury , Anna Mascellani Bergo , Jaroslav Havlik , Konstantinos Gerasimidis

{"title":"微量营养素补充影响健康成人肠道微生物组的组成和饮食起源功能","authors":"Shona Mckirdy , Athanasios Koutsos , Ben Nichols , Maris Anderson , Sumet Dhami , Chandrama Roy Chowdhury , Anna Mascellani Bergo , Jaroslav Havlik , Konstantinos Gerasimidis","doi":"10.1016/j.clnu.2025.06.020","DOIUrl":null,"url":null,"abstract":"<div><h3>Background & aims</h3><div>Studies <em>in-vitro</em> and in animals propose that vitamins and minerals can alter the human gut microbiome. Human trials replicating these findings are scarce or used micronutrient supplementation in supraphysiological doses. We explored the effect of multivitamin/multimineral (MVMM) supplementation on the gut microbiome, and how participant's background diet can modify this effect.</div></div><div><h3>Methods</h3><div>In a randomised, controlled, cross-over study, 28 healthy adults (68 % female, mean [SD], 33 [13] years]) received either a MVMM supplement containing dosages close to the UK dietary recommendations for 23 micronutrients (intervention period) or no supplement (control period), for 10-days, separated by a 15-day washout period. Participants' diet was recorded and replicated between the two periods. Faecal microbiota composition was profiled using 16S rRNA sequencing, metabolome with <sup>1</sup>H NMR, short chain fatty acids (SCFAs) with gas chromatography, and sulphide concentration with colorimetry. Associations between background diet and microbiome responses during MVMM supplementation were explored using machine learning.</div></div><div><h3>Results</h3><div>Following MVMM supplementation, the absolute abundance of <em>Lachnoclostridium</em> and <em>UCG_005</em> taxa were lower, while the concentrations of total SCFAs, specifically propionate and butyrate, and total sulphide were higher than the control period. Compared to baseline, MVMM supplementation decreased the absolute abundance of Desulfobacterota, Actinobacteriota, Bifidobacteriaceae, <em>Erysipelatoclostridiaceae</em> and Veillonellaceae, and increased the concentration of propionate. Background dietary intakes of saturated fat and total carbohydrates related with the MVMM-induced effect of decreasing Desulfobacterota. Likewise, the intakes of vitamins B2, B12, and E, along with iron, related with the effect of MVMM on decreasing <em>Erysipelatoclostridiaceae</em>. No changes were observed within the control period.</div></div><div><h3>Conclusion</h3><div>In healthy adults, short-term MVMM supplementation influences the gut microbiota composition and diet-originating microbial metabolites and potentially host health.</div></div><div><h3>Registration</h3><div>This trial was registered at clinicaltrials.gov as NCT05371704.</div></div>","PeriodicalId":10517,"journal":{"name":"Clinical nutrition","volume":"51 ","pages":"Pages 293-303"},"PeriodicalIF":7.4000,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Micronutrient supplementation influences the composition and diet-originating function of the gut microbiome in healthy adults\",\"authors\":\"Shona Mckirdy , Athanasios Koutsos , Ben Nichols , Maris Anderson , Sumet Dhami , Chandrama Roy Chowdhury , Anna Mascellani Bergo , Jaroslav Havlik , Konstantinos Gerasimidis\",\"doi\":\"10.1016/j.clnu.2025.06.020\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background & aims</h3><div>Studies <em>in-vitro</em> and in animals propose that vitamins and minerals can alter the human gut microbiome. Human trials replicating these findings are scarce or used micronutrient supplementation in supraphysiological doses. We explored the effect of multivitamin/multimineral (MVMM) supplementation on the gut microbiome, and how participant's background diet can modify this effect.</div></div><div><h3>Methods</h3><div>In a randomised, controlled, cross-over study, 28 healthy adults (68 % female, mean [SD], 33 [13] years]) received either a MVMM supplement containing dosages close to the UK dietary recommendations for 23 micronutrients (intervention period) or no supplement (control period), for 10-days, separated by a 15-day washout period. Participants' diet was recorded and replicated between the two periods. Faecal microbiota composition was profiled using 16S rRNA sequencing, metabolome with <sup>1</sup>H NMR, short chain fatty acids (SCFAs) with gas chromatography, and sulphide concentration with colorimetry. Associations between background diet and microbiome responses during MVMM supplementation were explored using machine learning.</div></div><div><h3>Results</h3><div>Following MVMM supplementation, the absolute abundance of <em>Lachnoclostridium</em> and <em>UCG_005</em> taxa were lower, while the concentrations of total SCFAs, specifically propionate and butyrate, and total sulphide were higher than the control period. Compared to baseline, MVMM supplementation decreased the absolute abundance of Desulfobacterota, Actinobacteriota, Bifidobacteriaceae, <em>Erysipelatoclostridiaceae</em> and Veillonellaceae, and increased the concentration of propionate. Background dietary intakes of saturated fat and total carbohydrates related with the MVMM-induced effect of decreasing Desulfobacterota. Likewise, the intakes of vitamins B2, B12, and E, along with iron, related with the effect of MVMM on decreasing <em>Erysipelatoclostridiaceae</em>. No changes were observed within the control period.</div></div><div><h3>Conclusion</h3><div>In healthy adults, short-term MVMM supplementation influences the gut microbiota composition and diet-originating microbial metabolites and potentially host health.</div></div><div><h3>Registration</h3><div>This trial was registered at clinicaltrials.gov as NCT05371704.</div></div>\",\"PeriodicalId\":10517,\"journal\":{\"name\":\"Clinical nutrition\",\"volume\":\"51 \",\"pages\":\"Pages 293-303\"},\"PeriodicalIF\":7.4000,\"publicationDate\":\"2025-06-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Clinical nutrition\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0261561425001748\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"NUTRITION & DIETETICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical nutrition","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0261561425001748","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"NUTRITION & DIETETICS","Score":null,"Total":0}

引用次数: 0

摘要

背景,目的体外和动物研究表明，维生素和矿物质可以改变人体肠道微生物群。重复这些发现的人体试验很少，或者使用超生理剂量的微量营养素补充剂。我们探讨了补充多种维生素/多种矿物质（MVMM）对肠道微生物组的影响，以及参与者的背景饮食如何改变这种影响。方法在一项随机、对照、交叉研究中，28名健康成年人（68%为女性，平均[SD]， 33岁]）接受了10天的MVMM补充剂，其中含有23种微量营养素的剂量接近英国膳食建议（干预期）或不补充（对照期），中间间隔15天的洗脱期。参与者的饮食被记录下来，并在两个时期之间复制。采用16S rRNA测序、1H NMR分析代谢组、气相色谱分析短链脂肪酸（SCFAs）、比色法分析硫化物浓度。在补充MVMM期间，使用机器学习探索背景饮食和微生物组反应之间的关系。结果添加MVMM后，Lachnoclostridium和UCG_005分类群的绝对丰度降低，总scfa浓度（尤其是丙酸和丁酸）和总硫化物浓度高于对照期。与基线相比，添加MVMM降低了Desulfobacterota、Actinobacteriota、Bifidobacteriaceae、丹毒杆菌科和Veillonellaceae的绝对丰度，并增加了丙酸浓度。背景：饱和脂肪和总碳水化合物的膳食摄入量与mmmm诱导的脱硫菌减少效应有关。同样，维生素B2、B12、E和铁的摄入量与MVMM减少丹毒的效果有关。在对照期内未观察到任何变化。结论在健康成人中，短期补充MVMM会影响肠道菌群组成和饮食源性微生物代谢物，并可能影响宿主健康。本试验在clinicaltrials.gov注册，注册号为NCT05371704。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Micronutrient supplementation influences the composition and diet-originating function of the gut microbiome in healthy adults

Background & aims

Studies in-vitro and in animals propose that vitamins and minerals can alter the human gut microbiome. Human trials replicating these findings are scarce or used micronutrient supplementation in supraphysiological doses. We explored the effect of multivitamin/multimineral (MVMM) supplementation on the gut microbiome, and how participant's background diet can modify this effect.

Methods

In a randomised, controlled, cross-over study, 28 healthy adults (68 % female, mean [SD], 33 [13] years]) received either a MVMM supplement containing dosages close to the UK dietary recommendations for 23 micronutrients (intervention period) or no supplement (control period), for 10-days, separated by a 15-day washout period. Participants' diet was recorded and replicated between the two periods. Faecal microbiota composition was profiled using 16S rRNA sequencing, metabolome with ¹H NMR, short chain fatty acids (SCFAs) with gas chromatography, and sulphide concentration with colorimetry. Associations between background diet and microbiome responses during MVMM supplementation were explored using machine learning.

Results

Following MVMM supplementation, the absolute abundance of Lachnoclostridium and UCG_005 taxa were lower, while the concentrations of total SCFAs, specifically propionate and butyrate, and total sulphide were higher than the control period. Compared to baseline, MVMM supplementation decreased the absolute abundance of Desulfobacterota, Actinobacteriota, Bifidobacteriaceae, Erysipelatoclostridiaceae and Veillonellaceae, and increased the concentration of propionate. Background dietary intakes of saturated fat and total carbohydrates related with the MVMM-induced effect of decreasing Desulfobacterota. Likewise, the intakes of vitamins B2, B12, and E, along with iron, related with the effect of MVMM on decreasing Erysipelatoclostridiaceae. No changes were observed within the control period.

Conclusion

In healthy adults, short-term MVMM supplementation influences the gut microbiota composition and diet-originating microbial metabolites and potentially host health.

Registration

This trial was registered at clinicaltrials.gov as NCT05371704.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Clinical nutrition 医学-营养学

CiteScore

14.10

自引率

6.30%

发文量

356

审稿时长

28 days

期刊介绍： Clinical Nutrition, the official journal of ESPEN, The European Society for Clinical Nutrition and Metabolism, is an international journal providing essential scientific information on nutritional and metabolic care and the relationship between nutrition and disease both in the setting of basic science and clinical practice. Published bi-monthly, each issue combines original articles and reviews providing an invaluable reference for any specialist concerned with these fields.