生物生产细胞系的3D生物打印

Q1 Computer Science
Laura Chastagnier , Lucie Essayan , Celine Thomann , Julia Niemann , Elisabeth Errazuriz-Cerda , Manon Laithier , Anne Baudouin , Christophe Marquette , Emma Petiot
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引用次数: 0

摘要

三维(3D)生物打印提供了一种革命性的方法来复制哺乳动物细胞培养的活体环境,为生物生产和组织工程提供了潜在的进步。在这项研究中,我们研究了四种哺乳动物细胞系(HEK、MDCK、CHO和Vero)在生物3D打印结构中的生长、代谢活性和结构组织。我们的研究结果表明,即使是高度选择的、永生化的细胞系,在3D环境中培养时,也能恢复更接近其原生组织的生理特征。我们观察到增殖动力学的显著变化,包括生长速率降低和发酵活性降低。实验设计(DOE)方法确定了关键的生物制造参数,如水凝胶微孔隙度和交联条件,这些参数可以调节3D基质中的细胞行为和增殖。这些发现突出了3D生物打印的潜力,不仅可以用于医疗应用,如再生医学和药物测试,还可以通过支持更高的细胞密度和代谢效率来增强生物生产过程。我们的工作强调了优化3D培养条件以模仿活体行为和提高生产力的重要性,为工业应用的生物打印结构的可扩展性提供了新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
3D bioprinting of bioproduction cell lines
Three-dimensional (3D) bioprinting presents a transformative approach to replicating vivo-like environments for mammalian cell cultures, offering potential advances in bioproduction and tissue engineering. In this study, we investigated the growth, metabolic activity, and structural organization of four mammalian cell lines (HEK, MDCK, CHO, and Vero) in 3D bioprinted constructs. Our results demonstrate that even highly selected, immortalized cell lines can regain physiological traits closer to their native tissue when cultured in 3D environments. We observed significant shifts in proliferation kinetics, including reduced growth rates and reduced fermentative activity. A Design of Experiment (DOE) approach identified critical biofabrication parameters—such as hydrogel microporosity and cross-linking conditions—that modulate cell behavior and proliferation in 3D matrices. These findings highlight the potential of 3D bioprinting not only for medical applications, such as regenerative medicine and drug testing, but also for enhancing bioproduction processes by supporting higher cell densities and metabolic efficiency. Our work underscores the importance of optimizing 3D culture conditions to mimic vivo-like behaviors and improve productivity, offering new insights into the scalability of bioprinted constructs for industrial applications.
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来源期刊
Bioprinting
Bioprinting Computer Science-Computer Science Applications
CiteScore
11.50
自引率
0.00%
发文量
72
审稿时长
68 days
期刊介绍: Bioprinting is a broad-spectrum, multidisciplinary journal that covers all aspects of 3D fabrication technology involving biological tissues, organs and cells for medical and biotechnology applications. Topics covered include nanomaterials, biomaterials, scaffolds, 3D printing technology, imaging and CAD/CAM software and hardware, post-printing bioreactor maturation, cell and biological factor patterning, biofabrication, tissue engineering and other applications of 3D bioprinting technology. Bioprinting publishes research reports describing novel results with high clinical significance in all areas of 3D bioprinting research. Bioprinting issues contain a wide variety of review and analysis articles covering topics relevant to 3D bioprinting ranging from basic biological, material and technical advances to pre-clinical and clinical applications of 3D bioprinting.
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