破译细胞因子动力学：急性中耳炎的分阶段免疫反应

IF 3.5 3区医学 Q3 IMMUNOLOGY

Microbial pathogenesis Pub Date : 2025-07-07 DOI:10.1016/j.micpath.2025.107884

Yingying Chen , Xiaolin Zhang , Yang Liu , Ziru Wei , Maoli Yi , Bo Li , Yanfei Wang , Hongchun Zhao , Tihua Zheng , Qingyin Zheng

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引用次数: 0

摘要

背景：急性中耳炎（AOM）在学龄前儿童中非常普遍，如果不治疗，可导致慢性中耳炎或严重并发症。AOM的发病机制与宿主的免疫反应密切相关，但介导这些免疫反应的细胞因子的表达谱仍不清楚。目的研究AOM免疫反应中涉及的细胞因子，并阐明其在疾病不同阶段的作用。方法通过鼓膜注射非分型流感嗜血杆菌（NTHi）诱导小鼠AOM。收集中耳组织进行分析。PCR阵列和蛋白阵列在感染后6和12小时(h)检测细胞因子。然后使用qPCR和Luminex多重检测技术跟踪细胞因子在各个关键时间点的表达，使我们能够推测它们在免疫过程中的作用。结果spcr Array在6 h鉴定出13个mRNA表达差异显著的细胞因子，Protein Array在12 h鉴定出9个mRNA表达差异的细胞因子，qPCR在关键时间点（6 h、12 h、1d、2 d、3 d和7 d）追踪mRNA表达，共鉴定出20个细胞因子。Luminex在关键时间点（12 h, 1 d， 2 d和5 d）检测到31个细胞因子，其中10个在前面提到过。细胞因子根据其表达趋势分为三种不同的类型：早期（n = 13），进展期（n = 20）和终末期（n = 4）细胞因子。结论细胞因子在AOM的各个阶段发挥着独特而关键的作用，在同一阶段高表达的细胞因子之间可能存在相互作用。

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Deciphering cytokine Dynamics: Staged immune responses in acute otitis media

Background

Acute otitis media (AOM) is highly prevalent among preschool children and can lead to chronic otitis media or severe complications if untreated. The pathogenesis of AOM is closely tied to the host's immune response, yet the expression profile of cytokines, which mediate these responses, remains poorly defined.

Purpose

This study aims to identify cytokines involved in AOM immune responses and to elucidate their roles at different stages of the disease.

Methods

We induced AOM in mice by injecting nontypeable Haemophilus influenzae (NTHi) through the tympanic membrane. Middle ear tissues were collected for analysis. PCR Array and Protein Array identified cytokines at 6 and 12 hours (h) post-infection. qPCR and Luminex multiplex detection technology were then used to track cytokine expression at various key time points, allowing us to speculate on their roles in the immune process.

Results

PCR Array identified 13 cytokines with significant mRNA differences at 6 h. Protein Array identified 9 differentially expressed cytokines at 12 h qPCR tracked mRNA expression at key time points (6 h, 12 h, 1day (d), 2 d, 3 d and 7 d), revealing a total of 20 cytokines. Luminex detected 31 cytokines at key time points (12 h 1 d, 2 d and 5 d), 10 of which were previously mentioned. Cytokines were classified into three distinct categories according to their expression trends: early-phase (n = 13), progressive-phase (n = 20), and terminal-phase (n = 4) cytokines.

Conclusion

Cytokines play distinct and crucial roles at each stage of AOM, with potential interactions among those highly expressed in the same stage.

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来源期刊

Microbial pathogenesis 医学-免疫学

CiteScore

7.40

自引率

2.60%

发文量

472

审稿时长

56 days

期刊介绍： Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports. Research Areas Include: -Pathogenesis -Virulence factors -Host susceptibility or resistance -Immune mechanisms -Identification, cloning and sequencing of relevant genes -Genetic studies -Viruses, prokaryotic organisms and protozoa -Microbiota -Systems biology related to infectious diseases -Targets for vaccine design (pre-clinical studies)