无金标准的猫狗弓形虫检测新方法与新方法的评价——贝叶斯潜类分析

IF 2.2 2区 农林科学 Q2 PARASITOLOGY
Deliah Tamsyn Winterfeld , Birgit Schauer , Majda Globokar , Nikola Pantchev , Susan Mouchantat , Franz Josef Conraths , Helge Kampen , Gereon Schares , Pavlo Maksimov , Jana Schulz
{"title":"无金标准的猫狗弓形虫检测新方法与新方法的评价——贝叶斯潜类分析","authors":"Deliah Tamsyn Winterfeld ,&nbsp;Birgit Schauer ,&nbsp;Majda Globokar ,&nbsp;Nikola Pantchev ,&nbsp;Susan Mouchantat ,&nbsp;Franz Josef Conraths ,&nbsp;Helge Kampen ,&nbsp;Gereon Schares ,&nbsp;Pavlo Maksimov ,&nbsp;Jana Schulz","doi":"10.1016/j.vetpar.2025.110544","DOIUrl":null,"url":null,"abstract":"<div><div>The commonly used detection method for <em>Toxocara canis</em> and <em>Toxocara cati</em> eggs in faecal samples is the sedimentation-flotation technique (SF). Based on this method, a sequential sieving protocol (SF-SSV) was developed to achieve a higher sensitivity in detecting <em>Toxocara</em> spp. eggs. In addition, two automated DNA extraction methods for faecal samples were applied. Faecal samples of cats (n = 125) and dogs (n = 175) were tested to detect <em>Toxocara</em> spp. with all four methods. Two populations were investigated; one was considered positive based on previous examinations (n = 120), and the other one represented a field population-based sample (n = 180). In general, the parasitological methods (SF, SF-SSV) had in all cases higher estimated sensitivities than the DNA detection methods. For both, cats and dogs, the SF-SSV had significantly higher sensitivities than the DNA detection methods. The estimates for specificity were above 90 % in each analysis, but lowest for the SF-SSV. By DNA detection, <em>T. cati</em>-specific methods tended to have higher diagnostic sensitivities than <em>T. canis</em>-specific methods, but not statistically significant. Traditional methods of evaluating diagnostic tests require a gold standard and a pool of appropriate reference samples. Since there is no gold standard for detecting <em>Toxocara</em> spp. in cats and dogs, Bayesian Latent Class Analysis (BLCA), a probabilistic approach estimating test performance, is a useful tool to estimate sensitivity and specificity based on the available data. This study highlights the challenge of obtaining suitable samples for model estimation, especially in field settings where <em>Toxocara</em> spp. prevalence is typically very low.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"338 ","pages":"Article 110544"},"PeriodicalIF":2.2000,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evaluation of established and new methods for the detection of Toxocara spp. in cats and dogs without a gold standard – A Bayesian latent class analysis\",\"authors\":\"Deliah Tamsyn Winterfeld ,&nbsp;Birgit Schauer ,&nbsp;Majda Globokar ,&nbsp;Nikola Pantchev ,&nbsp;Susan Mouchantat ,&nbsp;Franz Josef Conraths ,&nbsp;Helge Kampen ,&nbsp;Gereon Schares ,&nbsp;Pavlo Maksimov ,&nbsp;Jana Schulz\",\"doi\":\"10.1016/j.vetpar.2025.110544\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The commonly used detection method for <em>Toxocara canis</em> and <em>Toxocara cati</em> eggs in faecal samples is the sedimentation-flotation technique (SF). Based on this method, a sequential sieving protocol (SF-SSV) was developed to achieve a higher sensitivity in detecting <em>Toxocara</em> spp. eggs. In addition, two automated DNA extraction methods for faecal samples were applied. Faecal samples of cats (n = 125) and dogs (n = 175) were tested to detect <em>Toxocara</em> spp. with all four methods. Two populations were investigated; one was considered positive based on previous examinations (n = 120), and the other one represented a field population-based sample (n = 180). In general, the parasitological methods (SF, SF-SSV) had in all cases higher estimated sensitivities than the DNA detection methods. For both, cats and dogs, the SF-SSV had significantly higher sensitivities than the DNA detection methods. The estimates for specificity were above 90 % in each analysis, but lowest for the SF-SSV. By DNA detection, <em>T. cati</em>-specific methods tended to have higher diagnostic sensitivities than <em>T. canis</em>-specific methods, but not statistically significant. Traditional methods of evaluating diagnostic tests require a gold standard and a pool of appropriate reference samples. Since there is no gold standard for detecting <em>Toxocara</em> spp. in cats and dogs, Bayesian Latent Class Analysis (BLCA), a probabilistic approach estimating test performance, is a useful tool to estimate sensitivity and specificity based on the available data. This study highlights the challenge of obtaining suitable samples for model estimation, especially in field settings where <em>Toxocara</em> spp. prevalence is typically very low.</div></div>\",\"PeriodicalId\":23716,\"journal\":{\"name\":\"Veterinary parasitology\",\"volume\":\"338 \",\"pages\":\"Article 110544\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2025-07-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinary parasitology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0304401725001554\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary parasitology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0304401725001554","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PARASITOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

粪样中犬弓形虫和猫弓形虫卵的常用检测方法是沉淀浮选法(SF)。在此基础上,建立了顺序筛选方案(SF-SSV),以提高检测弓形虫卵的灵敏度。此外,还应用了两种自动提取粪便样本DNA的方法。采用四种方法对猫(n = 125)和狗(n = 175)的粪便样本进行弓形虫检测。调查了两个种群;其中一个根据以前的检查被认为是阳性的(n = 120),另一个代表基于现场人群的样本(n = 180)。总体而言,寄生虫学方法(SF, SF- ssv)在所有情况下都比DNA检测方法具有更高的估计灵敏度。对于猫和狗,SF-SSV的灵敏度明显高于DNA检测方法。每种分析的特异性估计都在90 %以上,但SF-SSV的特异性最低。通过DNA检测,犬绦虫特异性方法的诊断敏感性高于犬绦虫特异性方法,但差异无统计学意义。评估诊断测试的传统方法需要一个金标准和一个适当的参考样本库。由于检测猫和狗的弓形虫没有金标准,贝叶斯潜类分析(BLCA)是一种估计测试性能的概率方法,是基于现有数据估计灵敏度和特异性的有用工具。这项研究强调了获取适合模型估计的样本的挑战,特别是在弓形虫患病率通常很低的野外环境中。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of established and new methods for the detection of Toxocara spp. in cats and dogs without a gold standard – A Bayesian latent class analysis
The commonly used detection method for Toxocara canis and Toxocara cati eggs in faecal samples is the sedimentation-flotation technique (SF). Based on this method, a sequential sieving protocol (SF-SSV) was developed to achieve a higher sensitivity in detecting Toxocara spp. eggs. In addition, two automated DNA extraction methods for faecal samples were applied. Faecal samples of cats (n = 125) and dogs (n = 175) were tested to detect Toxocara spp. with all four methods. Two populations were investigated; one was considered positive based on previous examinations (n = 120), and the other one represented a field population-based sample (n = 180). In general, the parasitological methods (SF, SF-SSV) had in all cases higher estimated sensitivities than the DNA detection methods. For both, cats and dogs, the SF-SSV had significantly higher sensitivities than the DNA detection methods. The estimates for specificity were above 90 % in each analysis, but lowest for the SF-SSV. By DNA detection, T. cati-specific methods tended to have higher diagnostic sensitivities than T. canis-specific methods, but not statistically significant. Traditional methods of evaluating diagnostic tests require a gold standard and a pool of appropriate reference samples. Since there is no gold standard for detecting Toxocara spp. in cats and dogs, Bayesian Latent Class Analysis (BLCA), a probabilistic approach estimating test performance, is a useful tool to estimate sensitivity and specificity based on the available data. This study highlights the challenge of obtaining suitable samples for model estimation, especially in field settings where Toxocara spp. prevalence is typically very low.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Veterinary parasitology
Veterinary parasitology 农林科学-寄生虫学
CiteScore
5.30
自引率
7.70%
发文量
126
审稿时长
36 days
期刊介绍: The journal Veterinary Parasitology has an open access mirror journal,Veterinary Parasitology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. This journal is concerned with those aspects of helminthology, protozoology and entomology which are of interest to animal health investigators, veterinary practitioners and others with a special interest in parasitology. Papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites in all domesticated animals, fall within the scope of the journal. Papers of geographically limited (local) interest which are not of interest to an international audience will not be accepted. Authors who submit papers based on local data will need to indicate why their paper is relevant to a broader readership. Parasitological studies on laboratory animals fall within the scope of the journal only if they provide a reasonably close model of a disease of domestic animals. Additionally the journal will consider papers relating to wildlife species where they may act as disease reservoirs to domestic animals, or as a zoonotic reservoir. Case studies considered to be unique or of specific interest to the journal, will also be considered on occasions at the Editors'' discretion. Papers dealing exclusively with the taxonomy of parasites do not fall within the scope of the journal.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信