核小体间距可以微调高阶染色质组装

IF 14.7 1区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES
Lifeng Chen, M. Julia Maristany, Stephen E. Farr, Jinyue Luo, Bryan A. Gibson, Lynda K. Doolittle, Jorge R. Espinosa, Jan Huertas, Sy Redding, Rosana Collepardo-Guevara, Michael K. Rosen
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引用次数: 0

摘要

细胞染色质显示异质结构和动力学,控制不同核过程的性质。模型援引染色质纤维构象群的相分离作为调节体内染色质组织的机制。在这里,我们结合生物化学和分子动力学模拟来检查,在单碱基对分辨率,核小体间距如何控制染色质相分离。我们发现,当DNA连接体从25 bp延伸到30 bp时,例如10n + 5和10n(整数N) bp长度,染色质凝聚体的热力学稳定性降低,核小体的流动性增加。模拟表明,这是由于核小体在分子间和分子内的堆叠之间的权衡,分别由刚性的10n + 5和10n bp连接体所青睐。重塑剂可以通过移动核小体来诱导或抑制相分离,改变分子内和分子间堆叠的平衡。染色质固有的相分离能力使得压缩和动力学的微调,可能有助于体内染色质的异质组织。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Nucleosome spacing can fine-tune higher-order chromatin assembly

Nucleosome spacing can fine-tune higher-order chromatin assembly

Cellular chromatin displays heterogeneous structure and dynamics, properties that control diverse nuclear processes. Models invoke phase separation of conformational ensembles of chromatin fibers as a mechanism regulating chromatin organization in vivo. Here we combine biochemistry and molecular dynamics simulations to examine, at single base-pair resolution, how nucleosome spacing controls chromatin phase separation. We show that as DNA linkers extend from 25 bp to 30 bp, as exemplars of 10 N + 5 and 10 N (integer N) bp lengths, chromatin condensates become less thermodynamically stable and nucleosome mobility increases. Simulations reveal that this is due to trade-offs between inter- and intramolecular nucleosome stacking, favored by rigid 10 N + 5 and 10 N bp linkers, respectively. A remodeler can induce or inhibit phase separation by moving nucleosomes, changing the balance between intra- and intermolecular stacking. The intrinsic phase separation capacity of chromatin enables fine tuning of compaction and dynamics, likely contributing to heterogeneous chromatin organization in vivo.

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来源期刊
Nature Communications
Nature Communications Biological Science Disciplines-
CiteScore
24.90
自引率
2.40%
发文量
6928
审稿时长
3.7 months
期刊介绍: Nature Communications, an open-access journal, publishes high-quality research spanning all areas of the natural sciences. Papers featured in the journal showcase significant advances relevant to specialists in each respective field. With a 2-year impact factor of 16.6 (2022) and a median time of 8 days from submission to the first editorial decision, Nature Communications is committed to rapid dissemination of research findings. As a multidisciplinary journal, it welcomes contributions from biological, health, physical, chemical, Earth, social, mathematical, applied, and engineering sciences, aiming to highlight important breakthroughs within each domain.
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